EBF1 (NM_182708) Human Tagged ORF Clone

CAT#: RC238896

EBF1 (myc-DDK-tagged) - Human early B-cell factor 1 (EBF1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EBF1
• Synonyms: COE1; EBF; O/E-1; OLF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238896 representing NM_182708
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGGGATTCAGGAAAGCATCCAACGGAGTGGAAGCAGCATGAAGGAAGAGCCGCTGGGCAGCGGCA
  TGAACGCGGTGCGGACGTGGATGCAGGGCGCCGGGGTGCTGGACGCCAACACGGCGGCGCAGAGCGGGGT
  GGGTCTGGCCCGGGCTCACTTTGAGAAGCAGCCGCCTTCCAATCTGCGGAAATCCAACTTCTTCCACTTC
  GTCCTGGCCCTCTACGACAGACAGGGCCAGCCCGTGGAGATCGAGAGGACAGCGTTTGTGGGGTTCGTGG
  AGAAGGAAAAAGAAGCCAACAGCGAAAAGACCAATAACGGAATTCACTACCGGCTTCAGCTTCTCTACAG
  CAATGGGATAAGGACGGAGCAGGATTTCTACGTGCGCCTCATTGACTCCATGACAAAACAAGCCATAGTG
  TATGAAGGCCAAGACAAGAACCCAGAAATGTGCCGAGTCTTGCTCACACATGAGATCATGTGCAGGTTCT
  TCTTGAAATTTTTCCTCAAATGTAACCAAAATTGCCTAAAGAATGCGGGAAACCCACGTGACATGCGGAG
  ATTCCAGGTCGTGGTGTCTACGACAGTCAATGTGGATGGCCATGTCCTGGCAGTCTCTGATAACATGTTT
  GTCCATAATAATTCCAAGCATGGGCGGAGGGCTCGGAGGCTTGACCCCTCGGAAGCTACTCCCTGTATCA
  AAGCCATCAGCCCGAGTGAAGGATGGACGACGGGAGGTGCGACTGTGATCATCATAGGGGACAATTTCTT
  TGATGGGTTACAGGTCATATTCGGTACCATGCTGGTCTGGAGTGAGTTGATCACTCCTCATGCCATCCGT
  GTGCAGACCCCTCCTCGGCACATCCCTGGTGTTGTGGAAGTCACACTGTCCTACAAATCTAAGCAGTTCT
  GCAAAGGAACACCAGGCAGATTCATTTATACAGCGCTCAACGAACCCACCATCGATTATGGTTTCCAGAG
  GTTACAGAAGGTCATTCCTCGGCACCCTGGTGACCCTGAGCGTTTGCCAAAGGAAGTAATACTCAAAAGG
  GCTGCGGATCTGGTAGAAGCACTGTATGGGATGCCACACAACAACCAGGAAATCATTCTGAAGAGAGCGG
  CCGACATTGCCGAGGCCCTGTACAGTGTTCCCCGCAACCACAACCAACTCCCGGCCCTTGCTAACACCTC
  GGTCCACGCAGGGATGATGGGCGTGAATTCGTTCAGTGGACAACTGGCCGTGAATGTCTCCGAGGCATCA
  CAAGCCACCAATCAGGGTTTCACCCGCAACTCAAGCAGCGTATCACCACACGGGTACGTGCCGAGCACCA
  CTCCCCAGCAGACCAACTATAACTCCGTCACCACGAGCATGAACGGATACGGCTCTGCCGCAATGTCCAA
  TTTGGGCGGCTCCCCCACCTTCCTCAACGGCTCAGCTGCCAACTCCCCCTATGCCATAGTGCCATCCAGC
  CCCACCATGGCCTCCTCCACAAGCCTCCCCTCCAACTGCAGCAGCTCCTCGGGCATCTTCTCCTTCTCAC
  CAGCCAACATGGTCTCAGCCGTGAAACAGAAGAGTGCTTTCGCACCAGTCGTCAGACCCCAGACCTCCCC
  ACCTCCCACCTGCACCAGCACCAACGGGAACAGCCTGCAAGCGATATCTGGCATGATTGTTCCTCCTATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238896 representing NM_182708
  Red=Cloning site Green=Tags(s)
  
  MFGIQESIQRSGSSMKEEPLGSGMNAVRTWMQGAGVLDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHF
  VLALYDRQGQPVEIERTAFVGFVEKEKEANSEKTNNGIHYRLQLLYSNGIRTEQDFYVRLIDSMTKQAIV
  YEGQDKNPEMCRVLLTHEIMCRFFLKFFLKCNQNCLKNAGNPRDMRRFQVVVSTTVNVDGHVLAVSDNMF
  VHNNSKHGRRARRLDPSEATPCIKAISPSEGWTTGGATVIIIGDNFFDGLQVIFGTMLVWSELITPHAIR
  VQTPPRHIPGVVEVTLSYKSKQFCKGTPGRFIYTALNEPTIDYGFQRLQKVIPRHPGDPERLPKEVILKR
  AADLVEALYGMPHNNQEIILKRAADIAEALYSVPRNHNQLPALANTSVHAGMMGVNSFSGQLAVNVSEAS
  QATNQGFTRNSSSVSPHGYVPSTTPQQTNYNSVTTSMNGYGSAAMSNLGGSPTFLNGSAANSPYAIVPSS
  PTMASSTSLPSNCSSSSGIFSFSPANMVSAVKQKSAFAPVVRPQTSPPPTCTSTNGNSLQAISGMIVPPM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182708
• ORF Size: 1680 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182708.1, NM_182708.2, NP_874367.1
• RefSeq Size: 5189 bp
• RefSeq ORF: 1683 bp
• Locus ID: 1879
• Cytogenetics: 5q33.3
• Protein Families: Transcription Factors
• MW: 61.5 kDa
• Gene Summary: ''