NMDAR2C (GRIN2C) (NM_001278553) Human Tagged ORF Clone

CAT#: RC239716

GRIN2C (myc-DDK-tagged) - Human glutamate receptor, ionotropic, N-methyl D-aspartate 2C (GRIN2C), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRIN2C
• Synonyms: GluN2C; NMDAR2C; NR2C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239716 representing NM_001278553
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGGGGCCCTGGGGCCGGCCCTGTTGCTCACCTCGCTCTTCGGTGCCTGGGCAGGGCTGGGTCCGG
  GGCAGGGCGAGCAGGGCATGACGGTGGCCGTGGTGTTTAGCAGCTCAGGGCCGCCCCAGGCCCAGTTCCG
  TGCCCGCCTCACCCCCCAGAGCTTCCTGGACCTACCCCTGGAGATCCAGCCGCTCACAGTTGGGGTCAAC
  ACCACCAACCCCAGCAGCCTCCTCACCCAGATCTGCGGCCTCCTGGGTGCTGCCCACGTCCACGGCATTG
  TCTTTGAGGACAACGTGGACACCGAGGCGGTGGCCCAGATCCTTGACTTCATCTCCTCCCAGACCCATGT
  GCCCATCCTCAGCATCAGCGGAGGCTCTGCTGTGGTCCTCACCCCCAAGGAGCCGGGCTCCGCCTTCCTG
  CAGCTGGGCGTGTCCCTGGAGCAGCAGCTGCAGGTGCTGTTCAAGGTGCTGGAAGAGTACGACTGGAGCG
  CCTTCGCCGTCATCACCAGCCTGCACCCGGGCCACGCGCTCTTCCTGGAGGGCGTGCGCGCCGTCGCCGA
  CGCCAGCCACGTGAGTTGGCGGCTGCTGGACGTGGTCACGCTGGAGCTGGGCCCGGGAGGGCCGCGCGCG
  CGCACGCAGCGCCTGCTGCGCCAGCTCGACGCGCCCGTGTTTGTGGCCTACTGCTCGCGCGAGGAGGCCG
  AGGTGCTCTTCGCCGAGGCGGCGCAGGCCGGTCTGGTGGGGCCCGGCCACGTGTGGCTGGTGCCCAACCT
  GGCGCTGGGCAGCACCGATGCGCCCCCCGCCACCTTCCCCGTGGGCCTCATCAGCGTCGTCACCGAGAGC
  TGGCGCCTCAGCCTGCGCCAGAAGGTGCGCGACGGCGTGGCCATTCTGGCCCTGGGCGCCCACAGCTACT
  GGCGCCAGCATGGAACCCTGCCAGCCCCGGCCGGGGACTGCCGTGTTCACCCTGGGCCCGTCAGCCCTGC
  CCGGGAGGCCTTCTACAGGCACCTACTGAATGTCACCTGGGAGGGCCGAGACTTCTCCTTCAGCCCTGGT
  GGGTACCTGGTCCAGCCCACCATGGTGGTGATCGCCCTCAACCGGCACCGCCTCTGGGAGATGGTGGGGC
  GCTGGGAGCATGGCGTCCTATACATGAAGTACCCCGTGTGGCCTCGCTACAGTGCCTCTCTGCAGCCTGT
  GGTGGACAGTCGGCACCTGACGGTGGCCACGCTGGAAGAGCGGCCCTTTGTCATCGTGGAGAGCCCTGAC
  CCTGGCACAGGAGGCTGTGTCCCCAACACCGTGCCCTGCCGCAGGCAGAGCAACCACACCTTCAGCAGCG
  GGGACGTGGCCCCCTACACCAAGCTCTGCTGTAAGGGATTCTGCATCGACATCCTCAAGAAGCTGGCCAG
  AGTGGTCAAATTCTCCTACGACCTGTACCTGGTGACCAACGGCAAGCATGGCAAGCGGGTGCGCGGCGTA
  TGGAACGGCATGATTGGGGAGGTGTACTACAAGCGGGCAGACATGGCCATCGGCTCCCTCACCATCAATG
  AGGAACGCTCCGAGATCGTAGACTTCTCTGTACCCTTTGTGGAGACGGGCATCAGTGTGATGGTGGCTCG
  CAGCAATGGCACCGTCTCCCCCTCGGCCTTCTTGGAGCCATATAGCCCTGCAGTGTGGGTGATGATGTTT
  GTCATGTGCCTCACTGTGGTGGCCATCACCGTCTTCATGTTCGAGTACTTCAGCCCTGTCAGCTACAACC
  AGAACCTCACCAGAGGCAAGAAGTCCGGGGGCCCAGCTTTCACTATCGGCAAGTCCGTGTGGCTGCTGTG
  GGCGCTGGTCTTCAACAACTCAGTGCCCATCGAGAACCCGCGGGGCACCACCAGCAAGATCATGGTTCTG
  GTCTGGGCCTTCTTTGCTGTCATCTTCCTCGCCAGCTACACGGCCAACCTGGCCGCCTTCATGATCCAAG
  AGCAATACATCGACACTGTGTCGGGCCTCAGTGACAAGAAGTTTCAGCGGCCTCAAGATCAGTACCCACC
  TTTCCGCTTCGGCACGGTGCCCAACGGCAGCACGGAGCGGAACATCCGCAGTAACTACCGTGACATGCAC
  ACCCACATGGTCAAGTTCAACCAGCGCTCGGTGGAGGACGCGCTCACCAGCCTCAAGATGGGGAAGCTGG
  ATGCCTTCATCTATGATGCTGCTGTCCTCAACTACATGGCAGGCAAGGACGAGGGCTGCAAGCTGGTCAC
  CATTGGGTCTGGCAAGGTCTTTGCTACCACTGGCTACGGCATCGCCATGCAGAAGGACTCCCACTGGAAG
  CGGGCCATAGACCTGGCGCTCTTGCAGTTCCTGGGGGACGGAGAGACACAGAAACTGGAGACAGTGTGGC
  TCTCAGGGATCTGCCAGAATGAGAAGAACGAGGTGATGAGCAGCAAGCTGGACATCGACAACATGGCAGG
  CGTCTTCTACATGCTGCTGGTGGCCATGGGGCTGGCCCTGCTGGTCTTCGCCTGGGAGCACCTGGTCTAC
  TGGAAGCTGCGCCACTCGGTGCCCAACTCATCCCAGCTGGACTTCCTGCTGGCTTTCAGCAGGGTGGGTG
  CCCACCCCTCCCCACACAGGCCAAAGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239716 representing NM_001278553
  Red=Cloning site Green=Tags(s)
  
  MGGALGPALLLTSLFGAWAGLGPGQGEQGMTVAVVFSSSGPPQAQFRARLTPQSFLDLPLEIQPLTVGVN
  TTNPSSLLTQICGLLGAAHVHGIVFEDNVDTEAVAQILDFISSQTHVPILSISGGSAVVLTPKEPGSAFL
  QLGVSLEQQLQVLFKVLEEYDWSAFAVITSLHPGHALFLEGVRAVADASHVSWRLLDVVTLELGPGGPRA
  RTQRLLRQLDAPVFVAYCSREEAEVLFAEAAQAGLVGPGHVWLVPNLALGSTDAPPATFPVGLISVVTES
  WRLSLRQKVRDGVAILALGAHSYWRQHGTLPAPAGDCRVHPGPVSPAREAFYRHLLNVTWEGRDFSFSPG
  GYLVQPTMVVIALNRHRLWEMVGRWEHGVLYMKYPVWPRYSASLQPVVDSRHLTVATLEERPFVIVESPD
  PGTGGCVPNTVPCRRQSNHTFSSGDVAPYTKLCCKGFCIDILKKLARVVKFSYDLYLVTNGKHGKRVRGV
  WNGMIGEVYYKRADMAIGSLTINEERSEIVDFSVPFVETGISVMVARSNGTVSPSAFLEPYSPAVWVMMF
  VMCLTVVAITVFMFEYFSPVSYNQNLTRGKKSGGPAFTIGKSVWLLWALVFNNSVPIENPRGTTSKIMVL
  VWAFFAVIFLASYTANLAAFMIQEQYIDTVSGLSDKKFQRPQDQYPPFRFGTVPNGSTERNIRSNYRDMH
  THMVKFNQRSVEDALTSLKMGKLDAFIYDAAVLNYMAGKDEGCKLVTIGSGKVFATTGYGIAMQKDSHWK
  RAIDLALLQFLGDGETQKLETVWLSGICQNEKNEVMSSKLDIDNMAGVFYMLLVAMGLALLVFAWEHLVY
  WKLRHSVPNSSQLDFLLAFSRVGAHPSPHRPKF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278553
• ORF Size: 2619 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278553.1, NP_001265482.1
• RefSeq Size: 3269 bp
• RefSeq ORF: 2622 bp
• Locus ID: 2905
• Cytogenetics: 17q25.1
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Calcium signaling pathway, Long-term potentiation, Neuroactive ligand-receptor interaction
• MW: 96.4 kDa
• Gene Summary: 'This gene encodes a subunit of the N-methyl-D-aspartate (NMDA) receptor, which is a subtype of ionotropic glutamate receptor. NMDA receptors are found in the central nervous system, are permeable to cations and have an important role in physiological processes such as learning, memory, and synaptic development. The receptor is a tetramer of different subunits (typically heterodimer of subunit 1 with one or more of subunits 2A-D), forming a channel that is permeable to calcium, potassium, and sodium, and whose properties are determined by subunit composition. Alterations in the subunit composition of the receptor are associated with pathophysiological conditions such as Parkinson's disease, Alzheimer's disease, depression, and schizophrenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]'