PPM1G (NM_002707) Human Tagged ORF Clone

CAT#: RG200439

PPM1G (GFP-tagged) - Human protein phosphatase, Mg2+/Mn2+ dependent, 1G (PPM1G), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PPM1G
• Synonyms: MGC1675; MGC2870; PP2CG; PP2CGAMMA; PPP2CG
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200439 representing NM_002707
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGCCTACCTCTCCCAGCCCAACACGGTGAAGTGCTCCGGGGACGGGGTCGGCGCCCCGCGCCTGC
  CGCTGCCCTACGGCTTCTCCGCCATGCAAGGCTGGCGCGTCTCCATGGAGGATGCTCACAACTGTATTCC
  TGAGCTGGACAGTGAGACAGCCATGTTTTCTGTCTACGATGGACATGGAGGGGAGGAAGTTGCCTTGTAC
  TGTGCCAAATATCTTCCTGATATCATCAAAGATCAGAAGGCCTACAAGGAAGGCAAGCTACAGAAGGCTT
  TAGAAGATGCCTTCTTGGCTATTGACGCCAAATTGACCACTGAAGAAGTCATTAAAGAGCTGGCACAGAT
  TGCAGGGCGACCCACTGAGGATGAAGATGAAAAAGAAAAAGTAGCTGATGAAGATGATGTGGACAATGAG
  GAGGCTGCACTGCTGCATGAAGAGGCTACCATGACTATTGAAGAGCTGCTGACACGCTACGGGCAGAACT
  GTCACAAGGGCCCTCCCCACAGCAAATCTGGAGGTGGGACAGGCGAGGAACCAGGGTCCCAGGGCCTCAA
  TGGGGAGGCAGGACCTGAGGACTCAACTAGGGAAACTCCTTCACAAGAAAATGGCCCCACAGCCAAGGCC
  TACACAGGCTTTTCCTCCAACTCGGAACGTGGGACTGAGGCAGGCCAAGTTGGTGAGCCTGGCATTCCCA
  CTGGTGAGGCTGGGCCTTCCTGCTCTTCAGCCTCTGACAAGCTGCCTCGAGTTGCTAAGTCCAAGTTCTT
  TGAGGACAGTGAGGATGAGTCAGATGAGGCGGAGGAAGAAGAGGAAGACAGTGAGGAATGCAGCGAGGAA
  GAGGATGGCTACAGCAGTGAGGAGGCAGAGAATGAGGAAGATGAGGATGACACCGAGGAGGCTGAAGAGG
  ACGATGAAGAAGAAGAAGAAGAGATGATGGTGCCAGGGATGGAAGGCAAAGAGGAGCCTGGCTCTGACAG
  TGGTACAACAGCGGTGGTGGCCCTGATACGAGGGAAGCAGTTGATTGTAGCCAACGCAGGAGACTCTCGC
  TGTGTGGTATCTGAGGCTGGCAAAGCTTTAGACATGTCCTATGATCACAAACCAGAGGATGAAGTAGAAC
  TAGCACGCATCAAGAATGCTGGTGGCAAGGTCACCATGGATGGGCGAGTCAACGGGGGCCTCAACCTCTC
  CAGAGCCATTGGGGACCACTTCTATAAGAGAAACAAGAACCTGCCACCTGAGGAACAGATGATTTCAGCC
  CTTCCTGACATCAAGGTGCTGACTCTCACTGACGACCATGAATTCATGGTCATTGCCTGTGATGGCATCT
  GGAATGTGATGAGCAGCCAGGAAGTTGTAGATTTCATTCAATCAAAGATCAGCCAGCGTGATGAAAATGG
  GGAGCTTCGGTTATTGTCATCCATTGTGGAAGAGCTGCTGGATCAGTGCCTGGCACCAGACACTTCTGGG
  GATGGTACAGGGTGTGACAACATGACCTGCATCATCATTTGCTTCAAGCCCCGAAACACAGCAGAGCTCC
  AGCCAGAGAGTGGCAAGCGAAAACTAGAGGAGGTGCTCTCTACTGAGGGGGCTGAAGAAAATGGCAACAG
  CGACAAGAAGAAGAAGGCCAAGCGAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200439 representing NM_002707
  Red=Cloning site Green=Tags(s)
  
  MGAYLSQPNTVKCSGDGVGAPRLPLPYGFSAMQGWRVSMEDAHNCIPELDSETAMFSVYDGHGGEEVALY
  CAKYLPDIIKDQKAYKEGKLQKALEDAFLAIDAKLTTEEVIKELAQIAGRPTEDEDEKEKVADEDDVDNE
  EAALLHEEATMTIEELLTRYGQNCHKGPPHSKSGGGTGEEPGSQGLNGEAGPEDSTRETPSQENGPTAKA
  YTGFSSNSERGTEAGQVGEPGIPTGEAGPSCSSASDKLPRVAKSKFFEDSEDESDEAEEEEEDSEECSEE
  EDGYSSEEAENEEDEDDTEEAEEDDEEEEEEMMVPGMEGKEEPGSDSGTTAVVALIRGKQLIVANAGDSR
  CVVSEAGKALDMSYDHKPEDEVELARIKNAGGKVTMDGRVNGGLNLSRAIGDHFYKRNKNLPPEEQMISA
  LPDIKVLTLTDDHEFMVIACDGIWNVMSSQEVVDFIQSKISQRDENGELRLLSSIVEELLDQCLAPDTSG
  DGTGCDNMTCIIICFKPRNTAELQPESGKRKLEEVLSTEGAEENGNSDKKKKAKRD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002707
• ORF Size: 1638 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002707.3, NP_002698.1
• RefSeq Size: 2165 bp
• RefSeq ORF: 1640 bp
• Locus ID: 5496
• Cytogenetics: 2p23.3
• Domains: PP2C
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: 'The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]'