PPM1G (NM_002707) Human Untagged Clone

CAT#: SC118474

PPM1G (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1G (PPM1G), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPM1G
• Synonyms: MGC1675; MGC2870; PP2CG; PP2CGAMMA; PPP2CG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118474 sequence for NM_002707 edited (data generated by NextGen Sequencing)
  ATGGGTGCCTACCTCTCCCAGCCCAACACGGTGAAGTGCTCCGGGGACGGGGTCGGCGCC
  CCGCGCCTGCCGCTGCCCTACGGCTTCTCCGCCATGCAAGGCTGGCGCGTCTCCATGGAG
  GATGCTCACAACTGTATTCCTGAGCTGGACAGTGAGACAGCCATGTTTTCTGTCTACGAT
  GGACATGGAGGGGAGGAAGTTGCCTTGTACTGTGCCAAATATCTTCCTGATATCATCAAA
  GATCAGAAGGCCTACAAGGAAGGCAAGCTACAGAAGGCTTTAGAAGATGCCTTCTTGGCT
  ATTGACGCCAAATTGACCACTGAAGAAGTCATTAAAGAGCTGGCACAGATTGCAGGGCGA
  CCCACTGAGGATGAAGATGAAAAAGAAAAAGTAGCTGATGAAGATGATGTGGACAATGAG
  GAGGCTGCACTGCTGCATGAAGAGGCTACCATGACTATTGAAGAGCTGCTGACACGCTAC
  GGGCAGAACTGTCACAAGGGCCCTCCCCACAGCAAATCTGGAGGTGGGACAGGCGAGGAA
  CCAGGGTCCCAGGGCCTCAATGGGGAGGCAGGACCTGAGGACTCAACTAGGGAAACTCCT
  TCACAAGAAAATGGCCCCACAGCCAAGGCCTACACAGGCTTTTCCTCCAACTCGGAACGT
  GGGACTGAGGCAGGCCAAGTTGGTGAGCCTGGCATTCCCACTGGTGAGGCTGGGCCTTCC
  TGCTCTTCAGCCTCTGACAAGCTGCCTCGAGTTGCTAAGTCCAAGTTCTTTGAGGACAGT
  GAGGATGAGTCAGATGAGGCGGAGGAAGAAGAGGAAGACAGTGAGGAATGCAGCGAGGAA
  GAGGATGGCTACAGCAGTGAGGAGGCAGAGAATGAGGAAGATGAGGATGACACCGAGGAG
  GCTGAAGAGGACGATGAAGAAGAAGAAGAAGAGATGATGGTGCCAGGGATGGAAGGCAAA
  GAGGAGCCTGGCTCTGACAGTGGTACAACAGCGGTGGTGGCCCTGATACGAGGGAAGCAG
  TTGATTGTAGCCAACGCAGGAGACTCTCGCTGTGTGGTATCTGAGGCTGGCAAAGCTTTA
  GACATGTCCTATGATCACAAACCAGAGGATGAAGTAGAACTAGCACGCATCAAGAATGCT
  GGTGGCAAGGTCACCATGGATGGGCGAGTCAACGGGGGCCTCAACCTCTCCAGAGCCATT
  GGGGACCACTTCTATAAGAGAAACAAGAACCTGCCACCTGAGGAACAGATGATTTCAGCC
  CTTCCTGACATCAAGGTGCTGACTCTCACTGACGACCATGAATTCATGGTCATTGCCTGT
  GATGGCATCTGGAATGTGATGAGCAGCCAGGAAGTTGTAGATTTCATTCAATCAAAGATC
  AGCCAGCGTGATGAAAATGGGGAGCTTCGGTTATTGTCATCCATTGTGGAAGAGCTGCTG
  GATCAGTGCCTGGCACCAGACACTTCTGGGGATGGTACAGGGTGTGACAACATGACCTGC
  ATCATCATTTGCTTCAAGCCCCGAAACACAGCAGAGCTCCAGCCAGAGAGTGGCAAGCGA
  AAACTAGAGGAGGTGCTCTCTACTGAGGGGGCTGAAGAAAATGGCAACAGCGACAAGAAG
  AAGAAGGCCAAGCGAGACTAG
  
  Clone variation with respect to NM_002707.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002707 unedited
  GTTACCATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCCTGCACCTG
  AGGCCGCCGGCCAGCCGCCGCCTGGGTGCCTACCTCTCCCAGCCCACACGGTGAAGTGCT
  CCGGGGACGGGGTCGGCGCCCCGCGCCTGCCGCTGCCCTACGGCTTCTCCGCCATGCAAG
  GCTGGCGCGTCTCCATGGAGGATGCTCACAACTGTATTCCTGAGCTGGACAGTGAGACAG
  CCATGTTTTCTGTCTACGATGGACATGGAGGGGAGGAAGTTGCCTTGTACTGTGCCAAAT
  ATCTTCCTGATATCATCAAAGATCGGAAGGCCTACAAGGAAGGCAAGCTACAGAAGGCTT
  TAGAAGATGCCTTCTTGGCTATTGACGCCAAATTGACCACTGAAGAAGTCATTAAAGAGC
  TGGCACAGATTGCAGGGCGACCCACTGAGGATGAAGATGAAAAAGAAAAAGTAGCTGATG
  AAGATGATGTGGACAATGAGGAGGCTGCACTGCTGCATGAAGAGGCTACCATGACTATTG
  AAGAGCTGCTGACACGCTACGGGCAGAACTGTCACAAGGGCCCTCCCCACAGCAAATCTG
  GAGGTGGGACAGGCGAGGAACCAGGGTCCCAGGGCCTCAATGGGGAGGCAGGACCTGAGG
  ACTCAACTAGGGAAACTCCTTCACAAGAAAATGGCCCCACAGCCAAGGCCTACACAGGCT
  TTTCCTCCAACTCGGAACGTGGGACTGAGGCAGGCCAAGTTGGTGAGCCTGGCATTCCCA
  CTGGTGAGGCTGNGCCTTCCTGCTCTTCACCTCTGACAGCTGCCTCGAGTTGCTAAGTCC
  AGTTCTTTGAGGACAGTGAGATGAGTCANATGAGCGGAGAAGAANAGNANACAGTGAGAA
  TGCACGAGGANNAGATGCTACACN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002707 unedited
  TCCCGGTTTTGGCCGGGCCCACCNNTTAAATTACTTGGACGCGGCCGCATACTANATCGA
  GTTTTTTTTTTTTTTTTTTTTTTAGGTTTTGGCCTTTAGTCTGAAAAATGTTGATTGAAA
  GTGTACAACAGAGAGCGGGTGCAAGCGGCCGAGGGCCATGGAGCCGCCAATAAAAAAGAA
  TGTCCTTAAATAAAGTTCACAGAGTAAAAACCAGAACCGCCAGTCCTTCCCTCCAACACA
  ACAGAGCACAGGCACAGAACCGGTGATGAGCCCGAGGAGCATAGGCGGCTGGGAAGGACA
  GCAAAGGCTCCCGGCTGCAATGTGGAGGGAGAGCCCTTTTTGGAATGGGCGGAGTGAAGC
  CACCCAGCTCCCCCTGCACACCTCATACCCACTGCTAAGGCTAAAGGAAAAAGACAAAAC
  TCATTCTCAAGTCCGGAGGGCTCATAAAACAGTCTAGGTGGGCAGGGGTCTGGATGACTG
  CTAGTCTCGCTTGGGCTTCTTTTTTTTGGCGCTGTTGGCATTTTCTTCAACCCCCTCAGT
  AAAGAACACCCTCTTTAGTTTTTGCTTGCCACTCTCTGGCTGGAGCCTTTGTGTGTTCCG
  CGGCTTGAAGCATATGATTAAGCATGCTATGTTGTCAAAACTCGGTACATTCCCAAAAAG
  TCCTGGGGCCCAGATCTCGTTCCCGCACCTTCTTCCACAAGGGATGAACAAAACCCGAAA
  CCTCCCCATTTTCTTTACCCTGCGTTGATCCTTTGATTGGAAAGAAAACCTAAACAATTT
  CTGGGGTTGGTCCTTCCAATCTCTAAGAGGGTCCCACTGGGGCCTATGCCCTCTCAAATT
  CCTGGGCCCCCTATGCAAAACACCCCCATTCTTATTGTGAGGCACGCGCCTAAATTATTC
  GTTCCTCTCTAGGCGGGCGGTCCTCTTGTTTCTCCTTAATAAAAAGGGGCTCTCATGGGG
  TCCCGAGAAGGATGGAGGCCCCCCGTGCACTTCTCACTATCTTATGGTCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002707
• Insert Size: 2190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002707.3, NP_002698.1
• RefSeq Size: 2165 bp
• RefSeq ORF: 1641 bp
• Locus ID: 5496
• Cytogenetics: 2p23.3
• Domains: PP2C
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: 'The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]'
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants encode the same protein.