DPAGT1 (NM_001382) Human Tagged ORF Clone

CAT#: RG201787

DPAGT1 (GFP-tagged) - Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DPAGT1
• Synonyms: ALG7; CDG-Ij; CDG1J; CMS13; CMSTA2; D11S366; DGPT; DPAGT; DPAGT2; G1PT; GPT; UAGT; UGAT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201787 representing NM_001382
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGCCTTCTCGGAATTGCCCATGCCGCTGCTGATCAATTTGATCGTCTCGCTGCTGGGATTTGTGG
  CCACAGTCACCCTCATCCCGGCCTTCCGGGGCCACTTCATTGCTGCGCGCCTCTGTGGTCAGGACCTCAA
  CAAAACCAGCCGACAGCAGATCCCAGAATCCCAGGGAGTGATCAGCGGTGCTGTTTTCCTTATCATCCTC
  TTCTGCTTCATCCCTTTCCCCTTCCTGAACTGCTTTGTGAAGGAGCAGTGTAAGGCATTCCCCCACCATG
  AATTTGTGGCCCTGATAGGTGCCCTCCTTGCCATCTGCTGCATGATCTTCCTGGGCTTTGCGGATGATGT
  ACTGAATCTGCGCTGGCGCCATAAGCTGCTGCTACCTACAGCTGCCTCACTACCTCTCCTCATGGTCTAT
  TTCACCAACTTTGGCAACACGACCATTGTGGTGCCCAAGCCCTTCCGCCCGATACTTGGCCTGCATCTGG
  ACTTGGGAATCCTGTACTATGTCTACATGGGGCTGCTGGCAGTGTTCTGTACCAATGCCATCAATATCCT
  AGCAGGAATTAACGGCCTAGAGGCTGGCCAGTCACTAGTCATTTCTGCTTCCATCATTGTCTTCAACCTG
  GTAGAGTTGGAAGGTGATTGTCGGGATGATCATGTCTTTTCCCTCTACTTCATGATACCCTTTTTTTTCA
  CCACTTTGGGATTGCTCTACCACAACTGGTACCCATCACGGGTGTTTGTGGGAGATACCTTCTGTTACTT
  TGCTGGCATGACCTTTGCCGTGGTGGGCATCTTGGGACACTTCAGCAAGACCATGCTACTATTCTTCATG
  CCCCAGGTGTTCAACTTCCTCTACTCACTGCCTCAGCTCCTGCATATCATCCCCTGCCCTCGCCACCGCA
  TACCCAGACTCAATATCAAGACAGGCAAACTGGAGATGAGCTATTCCAAGTTCAAGACCAAGAGCCTCTC
  TTTCTTGGGCACCTTTATTTTAAAGGTGGCAGAGAGCCTCCAGCTGGTGACAGTACACCAGAGTGAGACT
  GAAGATGGTGAATTCACTGAATGTAACAACATGACCCTCATCAACTTGCTACTTAAAGTCCTTGGGCCCA
  TACATGAGAGAAACCTCACATTGCTCCTGCTGCTGCTGCAGATCCTGGGCAGTGCCATCACCTTCTCCAT
  TCGATATCAGCTCGTTCGACTCTTCTATGATGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201787 representing NM_001382
  Red=Cloning site Green=Tags(s)
  
  MWAFSELPMPLLINLIVSLLGFVATVTLIPAFRGHFIAARLCGQDLNKTSRQQIPESQGVISGAVFLIIL
  FCFIPFPFLNCFVKEQCKAFPHHEFVALIGALLAICCMIFLGFADDVLNLRWRHKLLLPTAASLPLLMVY
  FTNFGNTTIVVPKPFRPILGLHLDLGILYYVYMGLLAVFCTNAINILAGINGLEAGQSLVISASIIVFNL
  VELEGDCRDDHVFSLYFMIPFFFTTLGLLYHNWYPSRVFVGDTFCYFAGMTFAVVGILGHFSKTMLLFFM
  PQVFNFLYSLPQLLHIIPCPRHRIPRLNIKTGKLEMSYSKFKTKSLSFLGTFILKVAESLQLVTVHQSET
  EDGEFTECNNMTLINLLLKVLGPIHERNLTLLLLLLQILGSAITFSIRYQLVRLFYDV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001382
• ORF Size: 1224 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001382.2, NP_001373.2
• RefSeq Size: 2159 bp
• RefSeq ORF: 1227 bp
• Locus ID: 1798
• Cytogenetics: 11q23.3
• Domains: Glycos_transf_4
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: 'The protein encoded by this gene is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic reticulum. The congenital disorder of glycosylation type Ij is caused by mutation in the gene encoding this enzyme. [provided by RefSeq, Jul 2008]'