DPAGT1 (NM_001382) Human Untagged Clone

CAT#: SC320266

DPAGT1 (untagged)-Human dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) (DPAGT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DPAGT1
• Synonyms: ALG7; CDG-Ij; CDG1J; CMS13; CMSTA2; D11S366; DGPT; DPAGT; DPAGT2; G1PT; GPT; UAGT; UGAT
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001382.2 GGTGTCCCGACGGCGGCTCAAGTCAGAGTTGCTGGGTTTTGCTCAGATTGGTGTGGGAAG
  AGCCTGCCTGTGGGGAGCGGCCACTCCATACTGCTGAGGCCTCAGGACTGCTGCTCAGCT
  TGCCCGTTACCTGAAGAGGCGGCGGAGCCGGGCCCCTGACCGGTCACCATGTGGGCCTTC
  TCGGAATTGCCCATGCCGCTGCTGATCAATTTGATCGTCTCGCTGCTGGGATTTGTGGCC
  ACAGTCACCCTCATCCCGGCCTTCCGGGGCCACTTCATTGCTGCGCGCCTCTGTGGTCAG
  GACCTCAACAAAACCAGCCGACAGCAGATCCCAGAATCCCAGGGAGTGATCAGCGGTGCT
  GTTTTCCTTATCATCCTCTTCTGCTTCATCCCTTTCCCCTTCCTGAACTGCTTTGTGAAG
  GAGCAGTGTAAGGCATTCCCCCACCATGAATTTGTGGCCCTGATAGGTGCCCTCCTTGCC
  ATCTGCTGCATGATCTTCCTGGGCTTTGCGGATGATGTACTGAATCTGCGCTGGCGCCAT
  AAGCTGCTGCTACCTACAGCTGCCTCACTACCTCTCCTCATGGTCTATTTCACCAACTTT
  GGCAACACGACCATTGTGGTGCCCAAGCCCTTCCGCCCGATACTTGGCCTGCATCTGGAC
  TTGGGAATCCTGTACTATGTCTACATGGGGCTGCTGGCAGTGTTCTGTACCAATGCCATC
  AATATCCTAGCAGGAATTAACGGCCTAGAGGCTGGCCAGTCACTAGTCATTTCTGCTTCC
  ATCATTGTCTTCAACCTGGTAGAGTTGGAAGGTGATTGTCGGGATGATCATGTCTTTTCC
  CTCTACTTCATGATACCCTTTTTTTTCACCACTTTGGGATTGCTCTACCACAACTGGTAC
  CCATCACGGGTGTTTGTGGGAGATACCTTCTGTTACTTTGCTGGCATGACCTTTGCCGTG
  GTGGGCATCTTGGGACACTTCAGCAAGACCATGCTACTATTCTTCATGCCCCAGGTGTTC
  AACTTCCTCTACTCACTGCCTCAGCTCCTGCATATCATCCCCTGCCCTCGCCACCGCATA
  CCCAGACTCAATATCAAGACAGGCAAACTGGAGATGAGCTATTCCAAGTTCAAGACCAAG
  AGCCTCTCTTTCTTGGGCACCTTTATTTTAAAGGTGGCAGAGAGCCTCCAGCTGGTGACA
  GTACACCAGAGTGAGACTGAAGATGGTGAATTCACTGAATGTAACAACATGACCCTCATC
  AACTTGCTACTTAAAGTCCTTGGGCCCATACATGAGAGAAACCTCACATTGCTCCTGCTG
  CTGCTGCAGATCCTGGGCAGTGCCATCACCTTCTCCATTCGATATCAGCTCGTTCGACTC
  TTCTATGATGTCTGAGTCCCTTGATCATTGTCCTTTACCTCACAGTCTCTAGGATTCCTG
  ACTCAGGCTGACCTCTCTCTCTGGTCCCAGACTGCCTCCTTGCCCAGGCCTCTCTCACTC
  TTCATACTCCTCCAGATTTTGTTCTCAGCATTTTCCTTTCTCTGTGATCATTGGCATCCT
  GGGCGTTTCTTGCCCTCTGCTGACTACTGATTGGATTTTACCTATGGCTTTCTGCAACTT
  GCTACTCTCTCCCTCTCCATCCCATCTTTGCAGCCTCATAGGGTGGGATACAGCAGCTTT
  TTTTGCAGTTATCCACACTCACATTTCAGAGTCCTGACTCTCAAGGAACCACTGGTTTTT
  GGGATAGAACTTGGGCCAGGGCTAGGAACACAGGCTCCACGGTGACATGTCATTTGATTG
  TAAATTAAGTGTTCTGATTAGTAAGAACTAAGCAGGGGGCCACATGCTCTCAATGGAGAC
  AATAAAGTGTTGTCTTTTTCTTATAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001382
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001382.2, NP_001373.2
• RefSeq Size: 2159 bp
• RefSeq ORF: 1227 bp
• Locus ID: 1798
• Cytogenetics: 11q23.3
• Domains: Glycos_transf_4
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: 'The protein encoded by this gene is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. This enzyme belongs to the glycosyltransferase family 4. This protein is an integral membrane protein of the endoplasmic reticulum. The congenital disorder of glycosylation type Ij is caused by mutation in the gene encoding this enzyme. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform (a).