D Box Binding Protein (DBP) (NM_001352) Human Tagged ORF Clone

CAT#: RG202051

DBP (GFP-tagged) - Human D site of albumin promoter (albumin D-box) binding protein (DBP)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DBP
• Synonyms: DABP; taxREB302
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202051 representing NM_001352
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGGCCTGTGAGCGACAGGACCCCGGCCCCTCTGCTGCTGGGCGGCCCGGCCGGGACACCCCCTG
  GCGGGGGAGCGCTGCTTGGGTTGCGGAGCCTTCTGCAGGGGACCAGCAAGCCCAAAGAGCCGGCCAGCTG
  TCTCCTGAAGGAAAAGGAGCGCAAGGCGGCCCTGCCTGCAGCCACAACCCCTGGGCCAGGCCTGGAGACT
  GCGGGCCCGGCGGATGCCCCGGCTGGGGCAGTGGTGGGCGGAGGGTCCCCGCGGGGGCGCCCGGGGCCGG
  TGCCCGCCCCGGGTCTGTTGGCGCCACTGCTGTGGGAGCGCACGCTGCCGTTCGGCGATGTGGAGTACGT
  AGACCTGGACGCCTTCCTGCTGGAGCACGGGCTCCCGCCCAGCCCGCCGCCCCCCGGTGGCCCGTCGCCG
  GAGCCGTCGCCCGCGCGGACGCCCGCACCCTCCCCAGGGCCGGGTTCGTGCGGCTCGGCTTCCCCCCGCT
  CCTCTCCTGGGCACGCCCCCGCCCGGGCTGCCCTCGGGACCGCCAGCGGCCACCGCGCAGGCCTGACCTC
  TCGGGACACACCCAGCCCTGTGGACCCAGACACCGTGGAGGTGTTGATGACCTTTGAACCCGACCCAGCT
  GATCTTGCCCTATCAAGCATTCCTGGCCACGAGACCTTTGACCCTCGAAGACATCGCTTCTCAGAAGAGG
  AACTTAAGCCCCAGCCAATCATGAAGAAGGCAAGAAAAATCCAGGTGCCGGAGGAGCAGAAGGATGAGAA
  ATACTGGAGCCGGCGGTACAAGAACAACGAGGCAGCCAAGCGGTCCCGTGACGCCCGGCGGCTCAAGGAG
  AACCAGATATCGGTGCGGGCGGCCTTCCTGGAGAAGGAGAACGCCCTGCTGCGGCAGGAAGTTGTGGCCG
  TGCGCCAGGAGCTGTCCCACTACCGCGCCGTGCTGTCCCGATACCAGGCCCAGCACGGGGCCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202051 representing NM_001352
  Red=Cloning site Green=Tags(s)
  
  MARPVSDRTPAPLLLGGPAGTPPGGGALLGLRSLLQGTSKPKEPASCLLKEKERKAALPAATTPGPGLET
  AGPADAPAGAVVGGGSPRGRPGPVPAPGLLAPLLWERTLPFGDVEYVDLDAFLLEHGLPPSPPPPGGPSP
  EPSPARTPAPSPGPGSCGSASPRSSPGHAPARAALGTASGHRAGLTSRDTPSPVDPDTVEVLMTFEPDPA
  DLALSSIPGHETFDPRRHRFSEEELKPQPIMKKARKIQVPEEQKDEKYWSRRYKNNEAAKRSRDARRLKE
  NQISVRAAFLEKENALLRQEVVAVRQELSHYRAVLSRYQAQHGAL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001352
• ORF Size: 975 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001352.2, NP_001343.2
• RefSeq Size: 1478 bp
• RefSeq ORF: 978 bp
• Locus ID: 1628
• Cytogenetics: 19q13.33
• Domains: BRLZ
• Protein Families: Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a member of the PAR bZIP transcription factor family and binds to specific sequences in the promoters of several genes, such as albumin, CYP2A4, and CYP2A5. The encoded protein can bind DNA as a homo- or heterodimer and is involved in the regulation of some circadian rhythm genes. [provided by RefSeq, Jul 2014]'