TLE2 (NM_003260) Human Tagged ORF Clone

CAT#: RG202474

TLE2 (GFP-tagged) - Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TLE2
• Synonyms: ESG; ESG2; GRG2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202474 representing NM_003260
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCCCCAGGGAAGGCACCCGACCCCGCTCCAGTCCGGCCAGCCCTTCAAGTTCTCGATCTTGGAGA
  TCTGCGACCGCATCAAAGAAGAATTCCAGTTTCTTCAGGCTCAATACCACAGCCTCAAGCTAGAATGTGA
  GAAGCTGGCCAGCGAGAAGACGGAAATGCAGCGACATTATGTCATGTATTATGAGATGTCGTACGGGCTC
  AACATTGAAATGCATAAGCAGGCGGAGATTGTGAAGCGTCTGAGCGGTATCTGCGCTCAGATTATCCCCT
  TCCTGACCCAGGAGCATCAGCAGCAGGTGCTCCAGGCCGTAGAACGCGCCAAGCAGGTCACCGTGGGGGA
  GCTGAACAGCCTCATCGGGCAGCAGCTCCAGCCGCTGTCCCACCACGCACCCCCTGTGCCCCTCACCCCC
  CGCCCAGCCGGGCTGGTGGGCGGCAGTGCTACGGGGCTGCTTGCTCTGTCTGGAGCCCTGGCTGCCCAGG
  CTCAGCTGGCGGCGGCTGTCAAGGAGGACCGTGCGGGCGTGGAGGCCGAGGGGTCCAGAGTGGAGAGAGC
  CCCGAGCAGGAGTGCATCTCCCTCGCCCCCTGAGAGTCTCGTGGAGGAGGAGCGACCGAGTGGCCCTGGT
  GGTGGCGGGAAGCAGAGAGCAGATGAGAAGGAGCCATCAGGACCTTATGAAAGCGACGAAGACAAGAGTG
  ATTACAATCTGGTGGTGGACGAGGACCAACCCTCAGAGCCCCCCAGCCCGGCTACCACCCCCTGCGGAAA
  GGTACCCATCTGCATTCCTGCCCGTCGGGACCTGGTGGACAGTCCAGCCTCCTTGGCCTCTAGCCTTGGC
  TCACCGCTGCCTAGAGCCAAGGAGCTCATCCTGAATGACCTTCCCGCCAGCACTCCTGCCTCCAAATCCT
  GTGACTCCTCCCCGCCCCAGGACGCTTCCACCCCCGGGCCCAGCTCGGCCAGTCACCTCTGCCAGCTTGC
  TGCCAAGCCAGCACCTTCCACGGACAGCGTCGCCCTGAGGAGCCCCCTGACTCTGTCCAGTCCCTTCACC
  ACGTCCTTCAGCCTGGGCTCCCACAGCACTCTCAACGGAGACCTCTCCGTGCCCAGCTCCTACGTCAGCC
  TCCACCTGTCCCCCCAGGTCAGCAGCTCTGTGGTGTACGGACGCTCCCCCGTGATGGCATTTGAGTCTCA
  TCCCCATCTCCGAGGGTCATCCGTCTCTTCCTCCCTACCCAGCATCCCTGGGGGAAAGCCGGCCTACTCC
  TTCCACGTGTCTGCGGACGGGCAGATGCAGCCGGTTCCCTTCCCCTCGGATGCACTGGTAGGCGCGGGCA
  TCCCGCGGCACGCCCGGCAGCTGCACACGCTGGCCCATGGCGAGGTGGTCTGCGCGGTCACCATCAGCGG
  CTCCACACAGCATGTGTACACGGGCGGCAAGGGCTGTGTGAAGGTGTGGGACGTGGGCCAGCCTGGGGCC
  AAGACGCCCGTGGCCCAGCTCGACTGCCTGAACCGAGACAACTACATTCGTTCCTGCAAGTTGCTGCCGG
  ATGGCCGGAGTCTGATCGTGGGCGGTGAGGCCAGCACCTTGTCCATTTGGGACCTGGCGGCGCCCACCCC
  CCGTATCAAGGCCGAGCTGACTTCCTCAGCCCCAGCCTGCTACGCCCTGGCCGTCAGCCCCGACGCCAAG
  GTTTGCTTCTCCTGCTGCAGCGATGGCAACATTGTGGTCTGGGACCTGCAGAATCAGACTATGGTCAGGC
  AGTTCCAGGGCCACACGGACGGCGCCAGCTGCATTGATATTTCCGATTACGGCACTCGGCTCTGGACAGG
  GGGCCTGGACAACACGGTGCGCTGCTGGGACCTGCGGGAGGGCCGCCAGCTGCAGCAGCATGACTTCAGC
  TCCCAGATTTTCTCCCTGGGCCACTGCCCTAACCAGGACTGGCTGGCGGTCGGAATGGAGAGTAGCAACG
  TGGAGATCCTGCACGTCCGCAAGCCGGAGAAATACCAGCTGCACCTCCACGAGAGCTGCGTGCTGTCCCT
  GAAGTTTGCCTCCTGCGGACGGTGGTTTGTGAGCACCGGGAAGGACAACCTGCTCAACGCCTGGAGGACG
  CCGTACGGGGCCAGCATTTTCCAGTCCAAGGAGTCGTCCTCAGTCCTGAGTTGTGACATCTCCAGAAATA
  ACAAATACATCGTGACAGGCTCGGGGGACAAGAAGGCCACCGTGTATGAGGTGGTCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202474 representing NM_003260
  Red=Cloning site Green=Tags(s)
  
  MYPQGRHPTPLQSGQPFKFSILEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGL
  NIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGQQLQPLSHHAPPVPLTP
  RPAGLVGGSATGLLALSGALAAQAQLAAAVKEDRAGVEAEGSRVERAPSRSASPSPPESLVEEERPSGPG
  GGGKQRADEKEPSGPYESDEDKSDYNLVVDEDQPSEPPSPATTPCGKVPICIPARRDLVDSPASLASSLG
  SPLPRAKELILNDLPASTPASKSCDSSPPQDASTPGPSSASHLCQLAAKPAPSTDSVALRSPLTLSSPFT
  TSFSLGSHSTLNGDLSVPSSYVSLHLSPQVSSSVVYGRSPVMAFESHPHLRGSSVSSSLPSIPGGKPAYS
  FHVSADGQMQPVPFPSDALVGAGIPRHARQLHTLAHGEVVCAVTISGSTQHVYTGGKGCVKVWDVGQPGA
  KTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAVSPDAK
  VCFSCCSDGNIVVWDLQNQTMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLREGRQLQQHDFS
  SQIFSLGHCPNQDWLAVGMESSNVEILHVRKPEKYQLHLHESCVLSLKFASCGRWFVSTGKDNLLNAWRT
  PYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003260
• ORF Size: 2229 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003260.3, NP_003251.2
• RefSeq Size: 2579 bp
• RefSeq ORF: 2232 bp
• Locus ID: 7089
• Cytogenetics: 19p13.3
• Domains: WD40, TLE_N
• Protein Families: Transcription Factors
• Gene Summary: ''