TLE2 (NM_003260) Human Untagged Clone

CAT#: SC118085

TLE2 (untagged)-Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TLE2
• Synonyms: ESG; ESG2; GRG2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003260, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTACCCCCAGGGAAGGCACCCGACCCCGCTCCAGTCCGGCCAGCCCTTCAAGTTCTCGATCTTGGAGA
  TCTGCGACCGCATCAAAGAAGAATTCCAGTTTCTTCAGGCTCAATACCACAGCCTCAAGCTAGAATGTGA
  GAAGCTGGCCAGCGAGAAGACGGAAATGCAGCGACATTATGTCATGTATTATGAGATGTCGTACGGGCTC
  AACATTGAAATGCATAAGCAGGCGGAGATTGTGAAGCGTCTGAGCGGTATCTGCGCTCAGATTATCCCCT
  TCCTGACCCAGGAGCATCAGCAGCAGGTGCTCCAGGCCGTAGAACGCGCCAAGCAGGTCACCGTGGGGGA
  GCTGAACAGCCTCATCGGGCAGCAGCTCCAGCCGCTGTCCCACCACGCACCCCCTGTGCCCCTCACCCCC
  CGCCCAGCCGGGCTGGTGGGCGGCAGTGCTACGGGGCTGCTTGCTCTGTCTGGAGCCCTGGCTGCCCAGG
  CTCAGCTGGCGGCGGCTGTCAAGGAGGACCGTGCGGGCGTGGAGGCCGAGGGGTCCAGAGTGGAGAGAGC
  CCCGAGCAGGAGTGCATCTCCCTCGCCCCCTGAGAGTCTCGTGGAGGAGGAGCGACCGAGTGGCCCTGGT
  GGTGGCGGGAAGCAGAGAGCAGATGAGAAGGAGCCATCAGGACCTTATGAAAGCGACGAAGACAAGAGTG
  ATTACAATCTGGTGGTGGACGAGGACCAACCCTCAGAGCCCCCCAGCCCGGCTACCACCCCCTGCGGAAA
  GGTACCCATCTGCATTCCTGCCCGTCGGGACCTGGTGGACAGTCCAGCCTCCTTGGCCTCTAGCCTTGGC
  TCACCGCTGCCTAGAGCCAAGGAGCTCATCCTGAATGACCTTCCCGCCAGCACTCCTGCCTCCAAATCCT
  GTGACTCCTCCCCGCCCCAGGACGCTTCCACCCCCGGGCCCAGCTCGGCCAGTCACCTCTGCCAGCTTGC
  TGCCAAGCCAGCACCTTCCACGGACAGCGTCGCCCTGAGGAGCCCCCTGACTCTGTCCAGTCCCTTCACC
  ACGTCCTTCAGCCTGGGCTCCCACAGCACTCTCAACGGAGACCTCTCCGTGCCCAGCTCCTACGTCAGCC
  TCCACCTGTCCCCCCAGGTCAGCAGCTCTGTGGTGTACGGACGCTCCCCCGTGATGGCATTTGAGTCTCA
  TCCCCATCTCCGAGGGTCATCCGTCTCTTCCTCCCTACCCAGCATCCCTGGGGGAAAGCCGGCCTACTCC
  TTCCACGTGTCTGCGGACGGGCAGATGCAGCCGGTTCCCTTCCCCTCGGATGCACTGGTAGGCGCGGGCA
  TCCCGCGGCACGCCCGGCAGCTGCACACGCTGGCCCATGGCGAGGTGGTCTGCGCGGTCACCATCAGCGG
  CTCCACACAGCATGTGTACACGGGCGGCAAGGGCTGTGTGAAGGTGTGGGACGTGGGCCAGCCTGGGGCC
  AAGACGCCCGTGGCCCAGCTCGACTGCCTGAACCGAGACAACTACATTCGTTCCTGCAAGTTGCTGCCGG
  ATGGCCGGAGTCTGATCGTGGGCGGTGAGGCCAGCACCTTGTCCATTTGGGACCTGGCGGCGCCCACCCC
  CCGTATCAAGGCCGAGCTGACTTCCTCAGCCCCAGCCTGCTACGCCCTGGCCGTCAGCCCCGACGCCAAG
  GTTTGCTTCTCCTGCTGCAGCGATGGCAACATTGTGGTCTGGGACCTGCAGAATCAGACTATGGTCAGGC
  AGTTCCAGGGCCACACGGACGGCGCCAGCTGCATTGATATTTCCGATTACGGCACTCGGCTCTGGACAGG
  GGGCCTGGACAACACGGTGCGCTGCTGGGACCTGCGGGAGGGCCGCCAGCTGCAGCAGCATGACTTCAGC
  TCCCAGATTTTCTCCCTGGGCCACTGCCCTAACCAGGACTGGCTGGCGGTCGGAATGGAGAGTAGCAACG
  TGGAGATCCTGCACGTCCGCAAGCCGGAGAAATACCAGCTGCACCTCCACGAGAGCTGCGTGCTGTCCCT
  GAAGTTTGCCTCCTGCGGACGGTGGTTTGTGAGCACCGGGAAGGACAACCTGCTCAACGCCTGGAGGACG
  CCGTACGGGGCCAGCATTTTCCAGTCCAAGGAGTCGTCCTCAGTCCTGAGTTGTGACATCTCCAGAAATA
  ACAAATACATCGTGACAGGCTCGGGGGACAAGAAGGCCACCGTGTATGAGGTGGTCTACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003260 unedited
  AAATTTGTATACGACTACTATAGGGCGGCCGCGAATTCGCACGAGGCCGCTTCTCCCTCC
  TCCCTCTTTCTTCGGGCAGCCTCCCCACCACCCCACTTCAGCCTCCCCCACTCTTGCCGC
  CTCCATATCATCAAGCTCTGGTGGCGCCTGGGGGGCTTTTCGGATCGGCAGGATGTACCC
  CCAGGGAAGGCACCCGACCCCGCTCCAGTCCGGCCAGCCCTTCAAGTTCTCGATCTTGGA
  GATCTGCGACCGCATCAAAGAAGAATTCCAGTTTCTTCAGGCTCAATACCACAGCCTCAA
  GCTAGAATGTGAGAAGCTGGCCAGCGAGAAGACGGAAATGCAGCGACATTATGTCATGTA
  TTATGAGATGTCGTACGGGCTCAACATTGAAATGCATAAGCAGGCGGACATTGTGAAGCG
  TCTGAGCGGTATCTGCGCTCAGATTATCCCCTTCCTGACCCAGGAGCATCAGCAGCAGGT
  GCTCCAGGCCGTAGAACGCGCCAAGCAGGTCACCGTGGGGGAGCTGAACAGCCTCATCGG
  GCAGCAGCAGCTCCAGCCGCTGTCCCACCACGCACCCCCTGTGCCCCTCACCCCCCGCCC
  AGCCGGGCTGGTGGGCGGCAGTGCTACGGGGCTGCTTGCTCTGTCTGGAGCCCTGGCTGC
  CCAGGCTCAGCTGGCGGCGGCTGTCAAGGAGGACCGTGCGGGCGTGGAGGCCCGAGGGTC
  CAAAGTGGAGAGAGCCCCGAGCAGGAGTGCATCTCCCTCGCCCCCTGAGAGTCTCGTGGA
  GGGAGAGCGACCGAGTGGCCCTNGGTGGTGGCGGNNAGCANAGAGCANATGAGAAGGAGC
  CATCANGACCTTTATGAAGCGACGAAGACCAGAGTGATA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003260 unedited
  TGACCGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGTGATAGATACATTT
  TATTTCCACCGAGGTCCCCTGCCCATCGGCCCCCACATGCAGCAGGGGAAGGTGTGAAGC
  CGTTGGCCAGAGAGCAGATGGGATGTACGGTTCCTAGGCAGGGCTGGGAGGCGGCTGCTA
  GGATGTCTGTCCCGGCTGCTGATTCCCCTGGGAGTCTGGACTTCGGGTACAGGAAGGGGG
  GTCATGTCTCAGTAGACCACCTCATACACGGTGGCCTTCTTGTCCCCCGAGCCTGTCACG
  ATGTATTTGTTATTTCTGGAGATGTCACAACTCAGGACTGAGGACGACTCCTTGGACTGG
  AAAATGCTGGCCCCGTACGGCGTCCTCCAGGCGTTGAGCAGGTTGTCCTTCCCGGTGCTC
  ACAAACCACCGTCCGCAGGAGGCAAACTTCAGGGACAGCACGCAGCTCTCGTGGAGGTGC
  AGCTGGTATTTCTCCGGCTTGCGGACGTGCAGGATCTCCACGTTGCTACTCTCCATTCCG
  ACCGCCAGCCAGTCCTGGTTAGGGCAGTGGCCCAGGGAGAAAATCTGGGAGCTGAAGTCA
  TGCTGCTGCAGCTGGCGGCCCTCCCGCAGGTCCCAGCAGCGCACCGTGTTGTCCAGGCCC
  CCTGTCCAGAGCCGAGTGCCGTAATCGGAAATATCAATGCAGCTGGCGCCGTCCGTGTGG
  CCCTGGAACTGCCTGACCATAGTCTGATTCTGCAGGTCCCCAGACCACATGTTGCCATCG
  CTGCAGCAGGAGAAAGCAACCTTGGCGTCNGGGCTGACGGCCANGCGTAACAAGCTGGGG
  CTGANGAANNTCACTCGCTGCGCGNGGGGGGGGGGCCCCCCCAAGTTCCAATGGACACAG
  GGGCTGTCCTACCGCCCACATCAGACTCCGGCATTCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003260
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003260.3, NP_003251.2
• RefSeq Size: 2579 bp
• RefSeq ORF: 2232 bp
• Locus ID: 7089
• Cytogenetics: 19p13.3
• Domains: WD40, TLE_N
• Protein Families: Transcription Factors
• Gene Summary: ''
  Transcript Variant: This variant (1) uses an alternate in-frame splice site in the 5' coding region, compared to variant 4. It encodes isoform 1, which is shorter by an amino acid, compared to isoform 4.