Carboxypeptidase B2 (CPB2) (NM_001872) Human Tagged ORF Clone

CAT#: RG202800

  • TrueORF®

CPB2 (GFP-tagged) - Human carboxypeptidase B2 (plasma) (CPB2), transcript variant 1


  "NM_001872" in other vectors (6)

Reconstitution Protocol

USD 460.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol CPB2
Synonyms CPU; PCPB; TAFI
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG202800 representing NM_001872
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCTTTGCAGCCTTGCAGTCCTTGTACCCATTGTTCTCTTCTGTGAGCAGCATGTCTTCGCGTTTC
AGAGTGGCCAAGTTCTAGCTGCTCTTCCTAGAACCTCTAGGCAAGTTCAAGTTCTACAGAATCTTACTAC
AACATATGAGATTGTTCTCTGGCAGCCGGTAACAGCTGACCTTATTGTGAAGAAAAAACAAGTCCATTTT
TTTGTAAATGCATCTGATGTCGACAATGTGAAAGCCCATTTAAATGTGAGCGGAATTCCATGCAGTGTCT
TGCTGGCAGACGTGGAAGATCTTATTCAACAGCAGATTTCCAACGACACAGTCAGCCCCCGAGCCTCCGC
ATCGTACTATGAACAGTATCACTCACTAAATGAAATCTATTCTTGGATAGAATTTATAACTGAGAGGCAT
CCTGATATGCTTACAAAAATCCACATTGGATCCTCATTTGAGAAGTACCCACTCTATGTTTTAAAGGTTT
CTGGAAAAGAACAAGCAGCCAAAAATGCCATATGGATTGACTGTGGAATCCATGCCAGAGAATGGATCTC
TCCTGCTTTCTGCTTGTGGTTCATAGGCCATATAACTCAATTCTATGGGATAATAGGGCAATATACCAAT
CTCCTGAGGCTTGTGGATTTCTATGTTATGCCGGTGGTTAATGTGGATGGTTATGACTACTCATGGAAAA
AGAATCGAATGTGGAGAAAGAACCGTTCTTTCTATGCGAACAATCATTGCATCGGAACAGACCTGAATAG
GAACTTTGCTTCCAAACACTGGTGTGAGGAAGGTGCATCCAGTTCCTCATGCTCGGAAACCTACTGTGGA
CTTTATCCTGAGTCAGAACCAGAAGTGAAGGCAGTGGCTAGTTTCTTGAGAAGAAATATCAACCAGATTA
AAGCATACATCAGCATGCATTCATACTCCCAGCATATAGTGTTTCCATATTCCTATACACGAAGTAAAAG
CAAAGACCATGAGGAACTGTCTCTAGTAGCCAGTGAAGCAGTTCGTGCTATTGAGAAAACTAGTAAAAAT
ACCAGGTATACACATGGCCATGGCTCAGAAACCTTATACCTAGCTCCTGGAGGTGGGGACGATTGGATCT
ATGATTTGGGCATCAAATATTCGTTTACAATTGAACTTCGAGATACGGGCACATACGGATTCTTGCTGCC
GGAGCGTTACATCAAACCCACCTGTAGAGAAGCTTTTGCCGCTGTCTCTAAAATAGCTTGGCATGTCATT
AGGAATGTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG202800 representing NM_001872
Red=Cloning site Green=Tags(s)

MKLCSLAVLVPIVLFCEQHVFAFQSGQVLAALPRTSRQVQVLQNLTTTYEIVLWQPVTADLIVKKKQVHF
FVNASDVDNVKAHLNVSGIPCSVLLADVEDLIQQQISNDTVSPRASASYYEQYHSLNEIYSWIEFITERH
PDMLTKIHIGSSFEKYPLYVLKVSGKEQAAKNAIWIDCGIHAREWISPAFCLWFIGHITQFYGIIGQYTN
LLRLVDFYVMPVVNVDGYDYSWKKNRMWRKNRSFYANNHCIGTDLNRNFASKHWCEEGASSSSCSETYCG
LYPESEPEVKAVASFLRRNINQIKAYISMHSYSQHIVFPYSYTRSKSKDHEELSLVASEAVRAIEKTSKN
TRYTHGHGSETLYLAPGGGDDWIYDLGIKYSFTIELRDTGTYGFLLPERYIKPTCREAFAAVSKIAWHVI
RNV

TRTRRLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001872
ORF Size 1269 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq NM_001872.3, NP_001863.2
RefSeq Size 1766
RefSeq ORF 1272
Locus ID 1361
Domains Zn_carbOpept, Propep_M14
Protein Families Druggable Genome, Protease, Secreted Protein
Protein Pathways Complement and coagulation cascades
Gene Summary Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

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