Apc5 (ANAPC5) (NM_016237) Human Tagged ORF Clone

CAT#: RG203030

ANAPC5 (GFP-tagged) - Human anaphase promoting complex subunit 5 (ANAPC5), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ANAPC5
• Synonyms: APC5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203030 representing NM_016237
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCGTCCACGAGAGCCTCTACTTCAATCCCATGATGACCAATGGGGTTGTGCACGCCAATGTGT
  TCGGCATCAAGGACTGGGTGACGCCGTACAAGATCGCGGTGCTGGTGCTGCTGAACGAGATGAGCCGCAC
  AGGCGAGGGCGCCGTCAGCCTCATGGAGCGGCGGAGGCTCAACCAGCTGCTCCTGCCCCTGCTGCAGGGC
  CCAGATATTACACTGTCAAAACTTTACAAGTTAATTGAAGAGTCTTGTCCACAGCTGGCAAATTCAGTGC
  AGATCAGAATCAAACTGATGGCTGAAGGCGAGTTGAAGGATATGGAACAGTTTTTTGATGACCTTTCAGA
  TTCTTTCTCTGGAACTGAACCAGAGGTTCACAAAACAAGTGTAGTAGGTTTGTTTCTGCGTCACATGATC
  TTGGCCTACAGTAAGCTTTCTTTCAGCCAAGTGTTTAAACTGTACACTGCCCTTCAGCAGTACTTCCAGA
  ATGGTGAGAAAAAGACAGTGGAGGATGCTGATATGGAACTGACCAGTAGAGATGAGGGTGAAAGAAAAAT
  GGAAAAAGAAGAACTTGATGTATCTGTAAGAGAAGAGGAGGTATCTTGCAGTGGGCCTCTGTCCCAAAAA
  CAAGCAGAATTTTTTCTTTCTCAACAGGCTTCTTTGCTAAAGAATGATGAGACTAAGGCCCTCACTCCAG
  CTTCCTTGCAGAAGGAATTAAACAATTTGTTGAAATTTAATCCTGATTTTGCTGAAGCGCATTATCTCAG
  CTACTTAAACAACCTCCGTGTCCAAGATGTTTTCAGTTCAACACACAGTCTCCTCCATTATTTTGATCGT
  CTGATTCTTACCGGAGCCGAAAGCAAAAGTAATGGGGAAGAGGGCTATGGCCGGAGCTTGAGATACGCCG
  CTCTGAATCTTGCCGCCCTGCACTGCCGCTTCGGTCACTATCAACAGGCAGAGCTCGCCCTGCAGGAGGC
  AATTAGGATTGCCCAGGAGTCCAACGATCACGTGTGTCTCCAGCACTGTTTGAGCTGGCTTTATGTGCTG
  GGGCAGAAGAGATCCGATAGCTATGTTCTGCTGGAGCATTCTGTGAAGAAGGCAGTACATTTTGGGTTAC
  CGTACCTCGCCTCCCTGGGAATACAGTCCCTTGTTCAACAGAGAGCTTTTGCTGGGAAGACGGCAAACAA
  GCTGATGGATGCCCTAAAGGACTCCGACCTCCTGCACTGGAAACACAGCCTGTCAGAGCTCATCGATATC
  AGCATCGCACAGAAAACGGCCATCTGGAGGCTGTATGGCCGCAGCACCATGGCACTGCAACAGGCCCAGA
  TGTTGCTGAGCATGAACAGCCTGGAGGCGGTGAATGCGGGCGTGCAGCAGAACAACACAGAGTCCTTTGC
  TGTCGCACTCTGCCACCTCGCAGAGCTACACGCGGAGCAGGGCTGTTTTGCTGCAGCTTCTGAAGTGTTA
  AAGCACTTGAAGGAACGATTTCCGCCTAATAGTCAGCACGCCCAGTTATGGATGTTATGTGATCAAAAAA
  TACAGTTTGACAGAGCAATGAATGATGGCAAATATCATTTGGCTGATTCACTTGTTACAGGAATCACAGC
  TCTCAATAGCATAGAGGGTGTTTATAGGAAAGCGGTTGTATTACAAGCTCAGAACCAAATGTCAGAGGCA
  CATAAGCTTTTACAAAAATTGTTGGTTCATTGTCAGAAACTGAAGAACACAGAAATGGTGATCAGTGTCC
  TACTGTCCGTGGCAGAGCTGTACTGGCGATCTTCCTCCCCTACCATCGCGCTGCCCATGCTCCTGCAGGC
  TCTGGCCCTCTCCAAGGAGTACCGGTTACAGTACTTGGCCTCTGAAACAGTGCTGAACTTGGCTTTTGCG
  CAGCTCATTCTTGGAATCCCAGAACAGGCCTTAAGTCTTCTCCACATGGCCATCGAGCCCATCTTGGCTG
  ACGGGGCTATCCTGGACAAAGGTCGTGCCATGTTCTTAGTGGCCAAGTGCCAGGTGGCTTCAGCAGCTTC
  CTACGATCAGCCGAAGAAAGCAGAAGCTCTGGAGGCTGCCATCGAGAACCTCAATGAAGCCAAGAACTAT
  TTTGCAAAGGTTGACTGCAAAGAGCGCATCAGGGACGTCGTTTACTTCCAGGCCAGACTCTACCATACCC
  TGGGGAAGACCCAGGAGAGGAACCGGTGTGCGATGCTCTTCCGGCAGCTGCATCAGGAGCTGCCCTCTCA
  TGGGGTACCCTTGATAAACCATCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203030 representing NM_016237
  Red=Cloning site Green=Tags(s)
  
  MASVHESLYFNPMMTNGVVHANVFGIKDWVTPYKIAVLVLLNEMSRTGEGAVSLMERRRLNQLLLPLLQG
  PDITLSKLYKLIEESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMI
  LAYSKLSFSQVFKLYTALQQYFQNGEKKTVEDADMELTSRDEGERKMEKEELDVSVREEEVSCSGPLSQK
  QAEFFLSQQASLLKNDETKALTPASLQKELNNLLKFNPDFAEAHYLSYLNNLRVQDVFSSTHSLLHYFDR
  LILTGAESKSNGEEGYGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHCLSWLYVL
  GQKRSDSYVLLEHSVKKAVHFGLPYLASLGIQSLVQQRAFAGKTANKLMDALKDSDLLHWKHSLSELIDI
  SIAQKTAIWRLYGRSTMALQQAQMLLSMNSLEAVNAGVQQNNTESFAVALCHLAELHAEQGCFAAASEVL
  KHLKERFPPNSQHAQLWMLCDQKIQFDRAMNDGKYHLADSLVTGITALNSIEGVYRKAVVLQAQNQMSEA
  HKLLQKLLVHCQKLKNTEMVISVLLSVAELYWRSSSPTIALPMLLQALALSKEYRLQYLASETVLNLAFA
  QLILGIPEQALSLLHMAIEPILADGAILDKGRAMFLVAKCQVASAASYDQPKKAEALEAAIENLNEAKNY
  FAKVDCKERIRDVVYFQARLYHTLGKTQERNRCAMLFRQLHQELPSHGVPLINHL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016237
• ORF Size: 2265 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016237.3, NP_057321.2
• RefSeq Size: 2490
• RefSeq ORF: 2268
• Locus ID: 51433
• Domains: TPR
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008]