ZNF31 (ZSCAN20) (NM_145238) Human Tagged ORF Clone

CAT#: RG203034

ZSCAN20 (GFP-tagged) - Human zinc finger and SCAN domain containing 20 (ZSCAN20). Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZSCAN20
• Synonyms: KOX29; ZFP-31; ZNF31; ZNF360
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203034 ORF sequence, codon optimized.
  Due to the complexity of NM_145238, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGGCGCTCGAGCTGCAGGCTCAGGCTAGCCCTCAGCCAGAGCCTGAAGAGCTCCTGATTGTCA
  AGCTCGAGGAAGATTCTTGGGGCAGCGAGTCCAAGCTGTGGGAGAAGGACCGCGGAAGCGTGAGCGGCCC
  TGAAGCCAGTCGCCAGCGCTTTCGGCAGTTTCAATACAGGGATGCGGCCGGACCCCACGAAGCGTTTTCC
  CAGCTTTGGGCGCTTTGTTGCAGGTGGCTGAGGCCTGAAATAAGGCTCAAGGAACAGATTCTTGAACTGC
  TCGTGCTGGAGCAGTTTCTCACAATTTTGCCTCGAGAGGTTCAGACCTGGGTCCAAGCAAGACACCCAGA
  AAGTGGCGAAGAGGCTGTGGCTCTCGTCGAGGATTGGCATCGGGAAACTAGAACCGCGGGCCAGTCCGGA
  CTGGAACTGCACACCGAAGAAACGCGGCCACTTAAGACTGGTGAAGAAGCCCAGTCATTCCAGCTCCAGC
  CAGTTGATCCCTGGCCGGAGGGCCAGTCCCAGAAAAAGGGCGTGAAGAACACCTGTCCCGATCTGCCTAA
  TCATCTTAATGCCGAGGTCGCCCCGCAGCCATTGAAAGAATCTGCCGTGCTGACACCAAGAGTGCCCACA
  CTGCCGAAAATGGGATCCGTGGGGGACTGGGAAGTCACAGCCGAGAGCCAGGAAGCACTGGGTCCGGGTA
  AACACGCCGAGAAAGAACTGTGCAAGGACCCACCAGGTGATGACTGTGGCAACTCTGTTTGCCTGGGGGT
  GCCCGTGTCTAAACCATCCAACACTAGCGAAAAGGAACAGGGACCGGAGTTCTGGGGGCTTTCTCTTATT
  AACTCCGGGAAGAGGTCTACCGCGGATTACTCACTGGACAATGAACCAGCACAGGCCCTGACATGGCGAG
  ATTCAAGAGCTTGGGAGGAACAGTACCAGTGGGACGTCGAAGATATGAAGGTGTCAGGGGTGCACTGGGG
  ATATGAGGAAACCAAAACTTTTCTGGCCATTCTGTCCGAGTCCCCTTTTTCAGAGAAGCTGCGGACATGT
  CACCAAAATAGACAGGTGTACCGCGCTATCGCCGAGCAGTTGCGCGCACGCGGATTCTTGAGGACTCTTG
  AACAGTGCAGATATCGCGTAAAAAACTTGCTTCGCAACTACAGAAAAGCTAAGTCTAGCCACCCCCCTGG
  GACCTGCCCATTCTATGAGGAACTGGAAGCGCTGGTGCGAGCGCGAACTGCAATTCGCGCCACTGACGGG
  CCAGGAGAAGCCGTGGCGCTGCCCCGATTGGGGGATTCAGATGCCGAGATGGATGAGCAAGAGGAAGGAG
  GCTGGGATCCCGAAGAAATGGCCGAAGACTGTAATGGCGCAGGGCTCGTGAACGTAGAATCAACTCAGGG
  CCCTAGGATCGCGGGAGCCCCAGCACTCTTTCAGTCCCGCATCGCGGGGGTGCACTGGGGATACGAGGAG
  ACAAAGGCTTTTTTGGCCATTCTCTCTGAGAGCCCATTTTCCGAAAAGTTGCGAACGTGCCACCAAAATT
  CCCAAGTGTATCGCGCGATAGCTGAGAGGCTGTGTGCATTGGGGTTTCTCAGAACCCTGGAGCAGTGCCG
  GTACCGCTTTAAAAATTTGTTGCGCAGTTATAGGAAGGCCAAGTCATCTCACCCTCCAGGAACATGTCCA
  TTTTACGAAGAGCTGGATAGCTTGATGCGAGCTAGGGCCGCCGTCAGAGCAATGGGAACCGTAAGAGAGG
  CCGCTGGCCTTCCACGGTGCGGTCAGAGCTCCGCCGAAACTGATGCTCAGGAAGCCTGGGGCGAAGTGGC
  AAACGAGGACGCTGTGAAACCCTCCACACTGTGCCCCAAAGCCCCTGACATGGGGTTCGAAATGAGACAT
  GAGGACGAAGATCAGATATCAGAGCAGGACATATTCGAAGGGTTGCCGGGAGCGCTCAGCAAGTGTCCAA
  CCGAGGCGGTGTGCCAGCCTCTCGATTGGGGAGAGGACAGTGAAAATGAGAACGAGGACGAGGGGCAGTG
  GGGAAATCCTTCTCAGGAACAATGGCAGGAATCCTCAAGCGAGGAAGACTTGGAGAAATTGATAGACCAC
  CAAGGGCTGTATCTCGCAGAAAAGCCTTATAAGTGTGATACTTGCATGAAGAGTTTCTCCCGCAGCAGTC
  ATTTCATTGCTCACCAAAGGATTCATACCGGGGAGAAACCATACAAGTGCTTGGAATGCGGTAAAAATTT
  CAGCGATCGATCCAACCTGAACACTCACCAGAGGATTCACACCGGCGAAAAACCCTATAAATGCCTGGAA
  TGCGGGAAGTCATTCAGCGATCATTCCAATCTGATCACTCACCAGAGAATACATACGGGCGAAAAGCCAT
  ATAAATGCGGCGAGTGCTGGAAGTCTTTTAATCAAAGTAGCAACCTGCTTAAGCACCAGAGGATTCACCT
  CGGCGGCAATCCAGATCAATGTTCCGAGCCCGGAGGTAATTTCGCACAGAGTCCATCTTTCAGCGCTCAT
  TGGCGCAATAGCACCGAGGAGACTGCGCCTGAGCAACCCCAAAGCATCTCTAAAGATTTGAATTCCCCAG
  GACCTCACTCCACTAATAGTGGGGAGAAGCTGTATGAATGCTCCGAGTGTGGAAGGTCATTCTCTAAGTC
  CAGCGCACTCATCAGTCATCAGCGGATTCATACCGGCGAAAAGCCCTACGAGTGTGCGGAATGCGGGAAA
  TCATTTAGCAAAAGCAGCACCCTGGCTAATCACCAGAGAACACACACAGGAGAAAAGCCTTACAAATGCG
  TTGATTGCGGAAAGTGTTTCTCAGAGAGGTCTAAGCTGATAACACACCAGCGGGTTCACACCGGCGAGAA
  GCCCTATAAGTGCCTTGAGTGCGGGAAGTTCTTTCGAGATCGGTCAAATCTGATCACACATCAGCGAATA
  CATACTGGGGAGAAACCCTACAAGTGTCGGGAATGCGGAAAATGTTTTAATCAGTCTAGCTCTCTTATAA
  TCCACCAGCGAATCCACACCGGGGAAAAACCGTACAAATGCACGGAATGTGGAAAAGACTTTAACAACAG
  CAGCCATTTCTCTGCCCATAGGAGGACTCACGCCGGCGGCAAGGCTTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203034 representing NM_145238
  Red=Cloning site Green=Tags(s)
  
  MAMALELQAQASPQPEPEELLIVKLEEDSWGSESKLWEKDRGSVSGPEASRQRFRQFQYRDAAGPHEAFS
  QLWALCCRWLRPEIRLKEQILELLVLEQFLTILPREVQTWVQARHPESGEEAVALVEDWHRETRTAGQSG
  LELHTEETRPLKTGEEAQSFQLQPVDPWPEGQSQKKGVKNTCPDLPNHLNAEVAPQPLKESAVLTPRVPT
  LPKMGSVGDWEVTAESQEALGPGKHAEKELCKDPPGDDCGNSVCLGVPVSKPSNTSEKEQGPEFWGLSLI
  NSGKRSTADYSLDNEPAQALTWRDSRAWEEQYQWDVEDMKVSGVHWGYEETKTFLAILSESPFSEKLRTC
  HQNRQVYRAIAEQLRARGFLRTLEQCRYRVKNLLRNYRKAKSSHPPGTCPFYEELEALVRARTAIRATDG
  PGEAVALPRLGDSDAEMDEQEEGGWDPEEMAEDCNGAGLVNVESTQGPRIAGAPALFQSRIAGVHWGYEE
  TKAFLAILSESPFSEKLRTCHQNSQVYRAIAERLCALGFLRTLEQCRYRFKNLLRSYRKAKSSHPPGTCP
  FYEELDSLMRARAAVRAMGTVREAAGLPRCGQSSAETDAQEAWGEVANEDAVKPSTLCPKAPDMGFEMRH
  EDEDQISEQDIFEGLPGALSKCPTEAVCQPLDWGEDSENENEDEGQWGNPSQEQWQESSSEEDLEKLIDH
  QGLYLAEKPYKCDTCMKSFSRSSHFIAHQRIHTGEKPYKCLECGKNFSDRSNLNTHQRIHTGEKPYKCLE
  CGKSFSDHSNLITHQRIHTGEKPYKCGECWKSFNQSSNLLKHQRIHLGGNPDQCSEPGGNFAQSPSFSAH
  WRNSTEETAPEQPQSISKDLNSPGPHSTNSGEKLYECSECGRSFSKSSALISHQRIHTGEKPYECAECGK
  SFSKSSTLANHQRTHTGEKPYKCVDCGKCFSERSKLITHQRVHTGEKPYKCLECGKFFRDRSNLITHQRI
  HTGEKPYKCRECGKCFNQSSSLIIHQRIHTGEKPYKCTECGKDFNNSSHFSAHRRTHAGGKAS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145238
• ORF Size: 3129 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145238.3, NP_660281.2
• RefSeq Size: 4243 bp
• RefSeq ORF: 3132 bp
• Locus ID: 7579
• Cytogenetics: 1p35.1
• Domains: myb_DNA-binding, LER, zf-C2H2
• Protein Families: Transcription Factors
• Gene Summary: ''