SIKE1 (NM_025073) Human Tagged ORF Clone

CAT#: RG203262

SIKE1 (GFP-tagged) - Human suppressor of IKBKE 1 (SIKE1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SIKE1
• Synonyms: SIKE
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203262 representing NM_025073
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTGCACCATCGAGAAGATCCTGACAGACGCCAAGACGCTGCTGGAGAGGCTACGGGAGCACGATG
  CGGCCGCCGAGTCGCTGGTGGATCAGTCGGCGGCGCTGCACCGGCGGGTAGCAGCTATGCGGGAGGCGGG
  GACAGCGCTTCCGGACCAGTATCAAGAGGATGCATCCGATATGAAGGACATGTCCAAATACAAACCTCAC
  ATTCTGCTGTCCCAAGAGAACACACAGATTAGAGACTTGCAACAGGAAAACAGAGAGCTATGGATTTCCT
  TGGAGGAACACCAGGATGCTTTGGAACTTATCATGAGCAAATATCGGAAACAGATGTTACAGTTAATGGT
  TGCTAAAAAAGCGGTGGATGCTGAACCAGTCCTGAAAGCTCACCAGTCTCACTCTGCAGAAATTGAGAGT
  CAGATTGACAGAATCTGTGAAATGGGAGAAGTGATGAGGAAAGCAGTTCAGGTGGATGATGACCAGTTTT
  GTAAGATTCAGGAAAAATTAGCCCAATTAGAGCTTGAAAATAAGGAACTTCGAGAATTATTGTCCATCAG
  CAGTGAGTCTCTTCAAGCCAGAAAGGAAAACTCAATGGACACTGCTTCCCAAGCCATCAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203262 representing NM_025073
  Red=Cloning site Green=Tags(s)
  
  MSCTIEKILTDAKTLLERLREHDAAAESLVDQSAALHRRVAAMREAGTALPDQYQEDASDMKDMSKYKPH
  ILLSQENTQIRDLQQENRELWISLEEHQDALELIMSKYRKQMLQLMVAKKAVDAEPVLKAHQSHSAEIES
  QIDRICEMGEVMRKAVQVDDDQFCKIQEKLAQLELENKELRELLSISSESLQARKENSMDTASQAIK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025073
• ORF Size: 621 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_025073.1, NP_079349.1
• RefSeq Size: 2209
• RefSeq ORF: 624
• Locus ID: 80143
• Protein Pathways: RIG-I-like receptor signaling pathway
• Gene Summary: SIKE interacts with IKK-epsilon (IKBKE; MIM 605048) and TBK1 (MIM 604834) and acts as a suppressor of TLR3 (MIM 603029) and virus-triggered interferon activation pathways (Huang et al., 2005 [PubMed 16281057]). [supplied by OMIM, Mar 2008]