CBX8 (NM_020649) Human Tagged ORF Clone

CAT#: RG203417

CBX8 (GFP-tagged) - Human chromobox homolog 8 (CBX8)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CBX8
• Synonyms: PC3; RC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203417 representing NM_020649
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTTTCAGCGGTGGGGGAGCGGGTGTTCGCGGCCGAAGCCCTCCTGAAGCGGCGCATACGGAAAG
  GACGCATGGAATACCTCGTGAAATGGAAGGGATGGTCGCAGAAGTACAGCACATGGGAACCGGAGGAAAA
  CATCCTGGATGCTCGCTTGCTCGCAGCCTTTGAGGAAAGGGAAAGAGAGATGGAGCTCTATGGCCCCAAA
  AAGCGTGGACCCAAGCCCAAAACCTTCCTCCTCAAAGCGCAGGCCAAGGCAAAGGCCAAAACTTACGAGT
  TTCGAAGTGACTCAGCCAGGGGCATCCGGATCCCCTACCCTGGCCGCTCGCCCCAGGACCTGGCCTCCAC
  TTCCCGGGCCCGGGAGGGCCTTCGAAACATGGGTTTGTCCCCGCCAGCGAGCAGCACCAGCACCAGCAGC
  ACCTGCCGCGCAGAGGCCCCTCGGGACCGGGACCGAGACCGGGATAGGGACCGGGAGCGGGATCGAGAAA
  GGGAGAGGGAGCGAGAGAGGGAGCGGGAACGTGAGAGGGAACGAGAGCGGGGTACCAGCAGAGTGGATGA
  CAAGCCCAGCTCACCGGGGGACAGCTCGAAGAAGCGAGGCCCCAAGCCCCGGAAGGAGCTCCCGGACCCC
  TCACAGAGGCCCTTAGGCGAACCCAGCGCCGGCCTCGGAGAGTACCTCAAGGGCAGGAAGCTGGACGACA
  CCCCTTCCGGGGCAGGAAAGTTTCCAGCCGGCCACAGTGTGATCCAGCTGGCCCGAAGACAGGACTCGGA
  CCTGGTGCAGTGTGGTGTGACCAGCCCTAGCTCAGCTGAGGCCACGGGCAAACTGGCTGTGGACACCTTC
  CCGGCCAGGGTGATAAAGCACAGGGCTGCCTTCCTGGAGGCCAAAGGCCAGGGTGCCCTAGATCCCAATG
  GCACCCGGGTCCGACATGGCTCAGGCCCCCCCAGCTCTGGGGGGGGCCTGTACCGGGACATGGGGGCCCA
  GGGGGGAAGGCCCTCCCTCATCGCCAGGATCCCTGTGGCCAGAATCCTGGGGGACCCGGAGGAAGAGTCC
  TGGAGCCCCTCCCTGACTAACCTGGAGAAGGTGGTGGTCACGGACGTGACCTCAAACTTTTTGACCGTCA
  CCATTAAGGAAAGTAACACGGACCAAGGCTTTTTTAAAGAGAAAAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203417 representing NM_020649
  Red=Cloning site Green=Tags(s)
  
  MELSAVGERVFAAEALLKRRIRKGRMEYLVKWKGWSQKYSTWEPEENILDARLLAAFEEREREMELYGPK
  KRGPKPKTFLLKAQAKAKAKTYEFRSDSARGIRIPYPGRSPQDLASTSRAREGLRNMGLSPPASSTSTSS
  TCRAEAPRDRDRDRDRDRERDRERERERERERERERERERGTSRVDDKPSSPGDSSKKRGPKPRKELPDP
  SQRPLGEPSAGLGEYLKGRKLDDTPSGAGKFPAGHSVIQLARRQDSDLVQCGVTSPSSAEATGKLAVDTF
  PARVIKHRAAFLEAKGQGALDPNGTRVRHGSGPPSSGGGLYRDMGAQGGRPSLIARIPVARILGDPEEES
  WSPSLTNLEKVVVTDVTSNFLTVTIKESNTDQGFFKEKR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020649
• ORF Size: 1167 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020649.1, NP_065700.1
• RefSeq Size: 1542
• RefSeq ORF: 1170
• Locus ID: 57332
• Domains: CHROMO
• Protein Families: Transcription Factors
• Gene Summary: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. [UniProtKB/Swiss-Prot Function]