POLG2 (NM_007215) Human Tagged ORF Clone

CAT#: RG203462

POLG2 (GFP-tagged) - Human polymerase (DNA directed), gamma 2, accessory subunit (POLG2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: POLG2
• Synonyms: HP55; MTPOLB; PEOA4; POLB; POLG-BETA; POLGB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203462 representing NM_007215
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTCTCGTGTAGCCGTCAGGGCCTGCCATAAGGTCTGCAGGTGCCTGTTGTCTGGGTTTGGGGGTC
  GAGTAGATGCGGGGCAGCCGGAGCTGTTGACGGAAAGGAGTAGCCCCAAAGGAGGGCATGTGAAGTCGCA
  CGCGGAGCTCGAGGGGAACGGCGAGCACCCAGAAGCCCCCGGGTCTGGAGAGGGAAGCGAGGCGCTGTTA
  GAGATCTGTCAGAGAAGGCATTTCCTAAGTGGAAGCAAGCAGCAGCTTAGCCGGGATTCTCTTCTGAGTG
  GGTGCCACCCCGGCTTCGGACCCTTGGGCGTAGAGTTGCGGAAGAACCTGGCCGCAGAATGGTGGACCTC
  GGTGGTGGTGTTCAGGGAGCAGGTATTCCCGGTGGACGCCCTCCACCACAAACCAGGCCCTTTGCTACCC
  GGGGACAGTGCCTTCAGGTTAGTTTCTGCAGAAACTCTACGCGAAATCTTGCAAGACAAAGAGCTGAGTA
  AGGAACAGCTAGTAGCATTTCTTGAGAACGTATTAAAAACTTCTGGGAAACTACGGGAGAACCTTCTTCA
  CGGTGCCTTGGAACACTATGTTAATTGCCTGGATCTGGTAAACAAGAGGCTACCTTATGGCCTTGCTCAG
  ATTGGAGTGTGTTTTCATCCTGTTTTTGACACTAAGCAGATACGAAATGGTGTTAAAAGTATTGGTGAGA
  AGACTGAAGCTTCGTTAGTATGGTTTACTCCTCCGAGAACTTCAAACCAGTGGCTTGATTTCTGGTTACG
  TCATCGACTCCAGTGGTGGAGAAAGTTTGCCATGAGTCCATCTAACTTCAGCAGCAGTGACTGTCAGGAT
  GAAGAAGGCCGGAAAGGAAACAAACTTTACTACAATTTTCCCTGGGGAAAGGAGTTAATAGAAACCCTGT
  GGAACCTAGGAGATCACGAACTTTTACACATGTATCCTGGCAATGTGTCTAAATTACATGGCCGAGATGG
  ACGAAAAAATGTGGTTCCTTGTGTTCTCTCTGTAAATGGGGACCTAGACCGAGGCATGCTGGCCTACCTC
  TATGATTCTTTCCAGCTGACAGAGAACTCCTTTACAAGAAAGAAAAATCTTCATAGAAAGGTACTTAAAC
  TTCACCCTTGTTTAGCCCCTATTAAGGTTGCTTTGGATGTAGGAAGAGGCCCCACATTGGAACTAAGACA
  GGTTTGTCAAGGGCTATTTAATGAGTTACTAGAAAATGGGATTTCTGTGTGGCCTGCTTATTTGGAAACT
  ATGCAGTCCTCATTGGAACAACTTTATTCGAAGTATGATGAAATGAGTATTCTCTTCACAGTTTTGGTTA
  CTGAAACTACTTTGGAGAATGGATTAATACATCTGAGAAGCAGAGACACCACAATGAAGGAAATGATGCA
  TATATCCAAATTAAAAGACTTTTTGATTAAGTATATATCATCAGCTAAGAATGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203462 representing NM_007215
  Red=Cloning site Green=Tags(s)
  
  MRSRVAVRACHKVCRCLLSGFGGRVDAGQPELLTERSSPKGGHVKSHAELEGNGEHPEAPGSGEGSEALL
  EICQRRHFLSGSKQQLSRDSLLSGCHPGFGPLGVELRKNLAAEWWTSVVVFREQVFPVDALHHKPGPLLP
  GDSAFRLVSAETLREILQDKELSKEQLVAFLENVLKTSGKLRENLLHGALEHYVNCLDLVNKRLPYGLAQ
  IGVCFHPVFDTKQIRNGVKSIGEKTEASLVWFTPPRTSNQWLDFWLRHRLQWWRKFAMSPSNFSSSDCQD
  EEGRKGNKLYYNFPWGKELIETLWNLGDHELLHMYPGNVSKLHGRDGRKNVVPCVLSVNGDLDRGMLAYL
  YDSFQLTENSFTRKKNLHRKVLKLHPCLAPIKVALDVGRGPTLELRQVCQGLFNELLENGISVWPAYLET
  MQSSLEQLYSKYDEMSILFTVLVTETTLENGLIHLRSRDTTMKEMMHISKLKDFLIKYISSAKNV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007215
• ORF Size: 1455 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007215.2, NP_009146.2
• RefSeq Size: 1595
• RefSeq ORF: 1458
• Locus ID: 11232
• Domains: HGTP_anticodon
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Metabolic pathways
• Gene Summary: This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions. [provided by RefSeq, Sep 2009]