POLG2 (NM_007215) Human Tagged ORF Clone

CAT#: RC203462

POLG2 (Myc-DDK-tagged)-Human polymerase (DNA directed), gamma 2, accessory subunit (POLG2), nuclear gene encoding mitochondrial protein

Product Images

Western blot validation of overexpression lysate (Cat# LY416122) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203462 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified POLG2 protein (Cat# TP303462). The protein was produced from HEK293T cells transfected with POLG2 cDNA clone (Cat# RC203462) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POLG2
• Synonyms: HP55; MTPOLB; PEOA4; POLB; POLG-BETA; POLGB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203462 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTCTCGTGTAGCCGTCAGGGCCTGCCATAAGGTCTGCAGGTGCCTGTTGTCTGGGTTTGGGGGTC
  GAGTAGATGCGGGGCAGCCGGAGCTGTTGACGGAAAGGAGTAGCCCCAAAGGAGGGCATGTGAAGTCGCA
  CGCGGAGCTCGAGGGGAACGGCGAGCACCCAGAAGCCCCCGGGTCTGGAGAGGGAAGCGAGGCGCTGTTA
  GAGATCTGTCAGAGAAGGCATTTCCTAAGTGGAAGCAAGCAGCAGCTTAGCCGGGATTCTCTTCTGAGTG
  GGTGCCACCCCGGCTTCGGACCCTTGGGCGTAGAGTTGCGGAAGAACCTGGCCGCAGAATGGTGGACCTC
  GGTGGTGGTGTTCAGGGAGCAGGTATTCCCGGTGGACGCCCTCCACCACAAACCAGGCCCTTTGCTACCC
  GGGGACAGTGCCTTCAGGTTAGTTTCTGCAGAAACTCTACGCGAAATCTTGCAAGACAAAGAGCTGAGTA
  AGGAACAGCTAGTAGCATTTCTTGAGAACGTATTAAAAACTTCTGGGAAACTACGGGAGAACCTTCTTCA
  CGGTGCCTTGGAACACTATGTTAATTGCCTGGATCTGGTAAACAAGAGGCTACCTTATGGCCTTGCTCAG
  ATTGGAGTGTGTTTTCATCCTGTTTTTGACACTAAGCAGATACGAAATGGTGTTAAAAGTATTGGTGAGA
  AGACTGAAGCTTCGTTAGTATGGTTTACTCCTCCGAGAACTTCAAACCAGTGGCTTGATTTCTGGTTACG
  TCATCGACTCCAGTGGTGGAGAAAGTTTGCCATGAGTCCATCTAACTTCAGCAGCAGTGACTGTCAGGAT
  GAAGAAGGCCGGAAAGGAAACAAACTTTACTACAATTTTCCCTGGGGAAAGGAGTTAATAGAAACCCTGT
  GGAACCTAGGAGATCACGAACTTTTACACATGTATCCTGGCAATGTGTCTAAATTACATGGCCGAGATGG
  ACGAAAAAATGTGGTTCCTTGTGTTCTCTCTGTAAATGGGGACCTAGACCGAGGCATGCTGGCCTACCTC
  TATGATTCTTTCCAGCTGACAGAGAACTCCTTTACAAGAAAGAAAAATCTTCATAGAAAGGTACTTAAAC
  TTCACCCTTGTTTAGCCCCTATTAAGGTTGCTTTGGATGTAGGAAGAGGCCCCACATTGGAACTAAGACA
  GGTTTGTCAAGGGCTATTTAATGAGTTACTAGAAAATGGGATTTCTGTGTGGCCTGCTTATTTGGAAACT
  ATGCAGTCCTCATTGGAACAACTTTATTCGAAGTATGATGAAATGAGTATTCTCTTCACAGTTTTGGTTA
  CTGAAACTACTTTGGAGAATGGATTAATACATCTGAGAAGCAGAGACACCACAATGAAGGAAATGATGCA
  TATATCCAAATTAAAAGACTTTTTGATTAAGTATATATCATCAGCTAAGAATGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203462 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRSRVAVRACHKVCRCLLSGFGGRVDAGQPELLTERSSPKGGHVKSHAELEGNGEHPEAPGSGEGSEALL
  EICQRRHFLSGSKQQLSRDSLLSGCHPGFGPLGVELRKNLAAEWWTSVVVFREQVFPVDALHHKPGPLLP
  GDSAFRLVSAETLREILQDKELSKEQLVAFLENVLKTSGKLRENLLHGALEHYVNCLDLVNKRLPYGLAQ
  IGVCFHPVFDTKQIRNGVKSIGEKTEASLVWFTPPRTSNQWLDFWLRHRLQWWRKFAMSPSNFSSSDCQD
  EEGRKGNKLYYNFPWGKELIETLWNLGDHELLHMYPGNVSKLHGRDGRKNVVPCVLSVNGDLDRGMLAYL
  YDSFQLTENSFTRKKNLHRKVLKLHPCLAPIKVALDVGRGPTLELRQVCQGLFNELLENGISVWPAYLET
  MQSSLEQLYSKYDEMSILFTVLVTETTLENGLIHLRSRDTTMKEMMHISKLKDFLIKYISSAKNV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007215
• ORF Size: 1455 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007215.2, NM_007215.3, NP_009146.2
• RefSeq Size: 1607 bp
• RefSeq ORF: 1458 bp
• Locus ID: 11232
• Domains: HGTP_anticodon
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Metabolic pathways
• MW: 54.9 kDa
• Gene Summary: This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions. [provided by RefSeq, Sep 2009]