TFIIE beta (GTF2E2) (NM_002095) Human Tagged ORF Clone
CAT#: RG203477
- TrueORF®
GTF2E2 (GFP-tagged) - Human general transcription factor IIE, polypeptide 2, beta 34kDa (GTF2E2)
"NM_002095" in other vectors (5)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | GTF2E2 |
Synonyms | FE; TF2E2; TFIIE-B; TTD6 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG203477 representing NM_002095
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCAAGCCTGTTGAGAGAAAGGGAGCTGTTCAAAAAACGAGCTCTTTCTACTCCTGTAGTAGAAA AACGTTCAGCATCTTCTGAGTCATCATCATCATCGTCAAAGAAGAAGAAAACAAAGGTAGAACATGGAGG ATCGTCAGGCTCTAAACAAAATTCTGATCATAGCAATGGATCATTTAACTTGAAAGCTTTGTCAGGAAGC TCTGGATATAAGTTTGGTGTTCTTGCTAAGATTGTGAATTACATGAAGACACGGCATCAGCGAGGAGATA CGCATCCTCTAACCTTAGATGAAATTTTGGATGAAACACAACATTTAGATATTGGACTCAAGCAGAAACA ATGGCTAATGACTGAGGCTTTAGTCAACAATCCCAAAATTGAAGTAATAGATGGGAAGTATGCTTTCAAG CCCAAGTACAACGTGAGAGATAAGAAGGCCCTACTTAGGCTCTTAGATCAGCATGACCAGCGAGGATTAG GAGGAATTCTTTTAGAAGACATAGAAGAAGCACTGCCCAATTCCCAGAAAGCTGTCAAGGCTTTGGGGGA CCAGATACTATTTGTAAATCGTCCCGATAAGAAGAAAATACTTTTCTTCAATGATAAGAGCTGTCAGTTT TCTGTGGATGAAGAATTTCAGAAACTGTGGAGGAGTGTCACTGTAGATTCCATGGACGAGGAGAAAATTG AAGAATATCTGAAGCGACAGGGTATTTCTTCCATGCAGGAATCTGGACCAAAGAAAGTGGCCCCTATTCA GAGAAGGAAAAAGCCTGCTTCACAGAAAAAGCGACGCTTTAAGACTCATAACGAACACTTGGCTGGAGTG CTGAAGGATTACTCTGACATTACTTCCAGCAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203477 representing NM_002095
Red=Cloning site Green=Tags(s) MDPSLLRERELFKKRALSTPVVEKRSASSESSSSSSKKKKTKVEHGGSSGSKQNSDHSNGSFNLKALSGS SGYKFGVLAKIVNYMKTRHQRGDTHPLTLDEILDETQHLDIGLKQKQWLMTEALVNNPKIEVIDGKYAFK PKYNVRDKKALLRLLDQHDQRGLGGILLEDIEEALPNSQKAVKALGDQILFVNRPDKKKILFFNDKSCQF SVDEEFQKLWRSVTVDSMDEEKIEEYLKRQGISSMQESGPKKVAPIQRRKKPASQKKRRFKTHNEHLAGV LKDYSDITSSK TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_002095 |
ORF Size | 873 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002095.4, NP_002086.1 |
RefSeq Size | 1560 bp |
RefSeq ORF | 876 bp |
Locus ID | 2961 |
Cytogenetics | 8p12 |
Domains | TFIIE_beta |
Protein Families | Transcription Factors |
Protein Pathways | Basal transcription factors |
Gene Summary | 'The general transcription factor IIE (TFIIE) is part of the RNA polymerase II transcription initiation complex, recruiting TFIIH and being essential for promoter clearance by RNA polymerase II. TFIIE is a heterodimer (and sometimes heterotetramer) of alpha and beta subunits. The protein encoded by this gene represents the beta subunit of TFIIE. [provided by RefSeq, Jan 2017]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC118828 | GTF2E2 (untagged)-Human general transcription factor IIE, polypeptide 2, beta 34kDa (GTF2E2) |
USD 310.00 |
|
SC320510 | GTF2E2 (untagged)-Human general transcription factor IIE, polypeptide 2, beta 34kDa (GTF2E2) |
USD 310.00 |
|
RC203477 | GTF2E2 (Myc-DDK-tagged)-Human general transcription factor IIE, polypeptide 2, beta 34kDa (GTF2E2) |
USD 420.00 |
|
RC203477L3 | Lenti ORF clone of Human general transcription factor IIE, polypeptide 2, beta 34kDa (GTF2E2), Myc-DDK-tagged |
USD 620.00 |
|
RC203477L4 | Lenti ORF clone of Human general transcription factor IIE, polypeptide 2, beta 34kDa (GTF2E2), mGFP tagged |
USD 620.00 |
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