TFIIE beta (GTF2E2) (NM_002095) Human Untagged Clone

CAT#: SC320510

GTF2E2 (untagged)-Human general transcription factor IIE, polypeptide 2, beta 34kDa (GTF2E2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GTF2E2
• Synonyms: FE; TF2E2; TFIIE-B; TTD6
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002095.4 CGGGAAGTGGCGGGCGGAGTCCCGGGTCCAGTCGCCGCCTCAGCTACCGCCGCTGCCGCC
  GCCGCCGCCGCCACCGCCAGTGGTGAGACCCCGACCTGGCGGGTCAGCGCTGGGCGTGCG
  TGCGGGCAGGCGGGGGCGCTGACGAGAAGCAGGAAGAGGGTGCAGTGCCGGCGTGGGCGG
  CCGGCCGAGGCGGAGGCGCAGGAAGGGGGCGGCGAGTCGTGCGAGGCTGCCCTTCTCACT
  CAGCATTATGGATCCAAGCCTGTTGAGAGAAAGGGAGCTGTTCAAAAAACGAGCTCTTTC
  TACTCCTGTAGTAGAAAAACGTTCAGCATCTTCTGAGTCATCATCATCATCGTCAAAGAA
  GAAGAAAACAAAGGTAGAACATGGAGGATCGTCAGGCTCTAAACAAAATTCTGATCATAG
  CAATGGATCATTTAACTTGAAAGCTTTGTCAGGAAGCTCTGGATATAAGTTTGGTGTTCT
  TGCTAAGATTGTGAATTACATGAAGACACGGCATCAGCGAGGAGATACGCATCCTCTAAC
  CTTAGATGAAATTTTGGATGAAACACAACATTTAGATATTGGACTCAAGCAGAAACAATG
  GCTAATGACTGAGGCTTTAGTCAACAATCCCAAAATTGAAGTAATAGATGGGAAGTATGC
  TTTCAAGCCCAAGTACAACGTGAGAGATAAGAAGGCCCTACTTAGGCTCTTAGATCAGCA
  TGACCAGCGAGGATTAGGAGGAATTCTTTTAGAAGACATAGAAGAAGCACTGCCCAATTC
  CCAGAAAGCTGTCAAGGCTTTGGGGGACCAGATACTATTTGTAAATCGTCCCGATAAGAA
  GAAAATACTTTTCTTCAATGATAAGAGCTGTCAGTTTTCTGTGGATGAAGAATTTCAGAA
  ACTGTGGAGGAGTGTCACTGTAGATTCCATGGACGAGGAGAAAATTGAAGAATATCTGAA
  GCGACAGGGTATTTCTTCCATGCAGGAATCTGGACCAAAGAAAGTGGCCCCTATTCAGAG
  AAGGAAAAAGCCTGCTTCACAGAAAAAGCGACGCTTTAAGACTCATAACGAACACTTGGC
  TGGAGTGCTGAAGGATTACTCTGACATTACTTCCAGCAAATAGGGAACAGTTTTGCCCTG
  GAACAGAGTTACAGATACACAATCAAGAGTGTTCTTGCTGATGCTCGGGGTCTGAAGACT
  GTCTTCCTATCTGCTTCTTGCGGCTGAGGAGAGGAGCAGTTCAGTTTACAAAACAAGTGC
  AAATTACCAAACTCAAAGCTTATTTGAGTAGAATGGGCTCATGGGCAATGTGATGTTCCC
  TGTTAACCTTCTGTTACTCCCTGGGAGAAAGGCGCTGAGCGTGGCATGCAGGTGTCTTTG
  CTGTGTTTTTCTCCACTTCTAAATGGTTCCTGGTTCCTTTCTTCCTCGTTTGTTACTTTA
  GAGCAAGTTTGCCCATAGTCTTGAATGCAATATTTGTTTATTCCAAAAGAACATATTTAT
  AATAAAATCACTGTAGAAGGATTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAA
• Restriction Sites: Please inquire
• ACCN: NM_002095
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002095.4, NP_002086.1
• RefSeq Size: 1560 bp
• RefSeq ORF: 876 bp
• Locus ID: 2961
• Cytogenetics: 8p12
• Domains: TFIIE_beta
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: 'The general transcription factor IIE (TFIIE) is part of the RNA polymerase II transcription initiation complex, recruiting TFIIH and being essential for promoter clearance by RNA polymerase II. TFIIE is a heterodimer (and sometimes heterotetramer) of alpha and beta subunits. The protein encoded by this gene represents the beta subunit of TFIIE. [provided by RefSeq, Jan 2017]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).