TFIIE beta (GTF2E2) (NM_002095) Human Untagged Clone

CAT#: SC118828

GTF2E2 (untagged)-Human general transcription factor IIE, polypeptide 2, beta 34kDa (GTF2E2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GTF2E2
• Synonyms: FE; TF2E2; TFIIE-B; TTD6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002095, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATCCAAGCCTGTTGAGAGAAAGGGAGCTGTTCAAAAAACGAGCTCTTTCTACTCCTGTAGTAGAAA
  AACGTTCAGCATCTTCTGAGTCATCATCATCATCGTCAAAGAAGAAGAAAACAAAGGTAGAACATGGAGG
  ATCGTCAGGCTCTAAACAAAATTCTGATCATAGCAATGGATCATTTAACTTGAAAGCTTTGTCAGGAAGC
  TCTGGATATAAGTTTGGTGTTCTTGCTAAGATTGTGAATTACATGAAGACACGGCATCAGCGAGGAGATA
  CGCATCCTCTAACCTTAGATGAAATTTTGGATGAAACACAACATTTAGATATTGGACTCAAGCAGAAACA
  ATGGCTAATGACTGAGGCTTTAGTCAACAATCCCAAAATTGAAGTAATAGATGGGAAGTATGCTTTCAAG
  CCCAAGTACAACGTGAGAGATAAGAAGGCCCTACTTAGGCTCTTAGATCAGCATGACCAGCGAGGATTAG
  GAGGAATTCTTTTAGAAGACATAGAAGAAGCACTGCCCAATTCCCAGAAAGCTGTCAAGGCTTTGGGGGA
  CCAGATACTATTTGTAAATCGTCCCGATAAGAAGAAAATACTTTTCTTCAATGATAAGAGCTGTCAGTTT
  TCTGTGGATGAAGAATTTCAGAAACTGTGGAGGAGTGTCACTGTAGATTCCATGGACGAGGAGAAAATTG
  AAGAATATCTGAAGCGACAGGGTATTTCTTCCATGCAGGAATCTGGACCAAAGAAAGTGGCCCCTATTCA
  GAGAAGGAAAAAGCCTGCTTCACAGAAAAAGCGACGCTTTAAGACTCATAACGAACACTTGGCTGGAGTG
  CTGAAGGATTACTCTGACATTACTTCCAGCAAATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002095 unedited
  TATATTTGTATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCTAGCACCCGA
  TCGGGAAGTGGCGGGCGGAGTCCCGGGTCCAGTCGCCGCCTCAGCTACCGCCGCTGCCGC
  CGCCGCCGCCGCCACCGCCAGTGGTGAGACCCCGACCTGGCGGGTCAGCGCTGGGCGTGC
  GTGCGGGCAGGCGGGGGCGCTGACGAGAAGCAGGAAGAGGGTGCAGTGCCGGCGTGGGCG
  GCCGGCCGAGGCGGAGGCGCAGGAAGGGGGCGGCGAGTCGTGCGAGGCTGCCCTTCTCAC
  TCAGCATTATGGATCCAAGCCTGTTGAGAGAAAGGGAGCTGTTCAAAAAACGAGCTCTTT
  CTACTCCTGTAGTAGAAAAACGTTCAGCATCTTCTGAGTCATCATCATCATCGTCAAAGA
  AGAAGAAAACAAAGGTAGAACATGGAGGATCGTCAGGCTCTAAACAAAATTCTGATCATA
  GCAATGGATCATTTAACTTGAAAGCTTTGTCAGGAAGCTCTGGATATAAGTTTGGTGTTC
  TTGCTAAGATTGTGAATTACATGAAGACACGGCATCAGCGAGGAGATACGCATCCTCTAA
  CCTTAGATGAAATTTTGGATGAAACACAACATTTAGATATTGGACTCAAGCAGAAACAAT
  GGCTAATGACTGAGGCTTTAGTCAACAATCCCAAAATTGAAGTAATAGATGGGAAGTATG
  CTTTCAAGCCCAAGTACAACGTGAGAGATAAGAAGGCCCTACTTANGCTCTTAGATCAGC
  ATGACCAGCGAGGATTAGGGAGGATTCTTTTAGAAGACATAGAAGAAGCACTGCCCAATT
  CCCAGAAGCTGTCAGGGCTTTGGGGACAGATCTATTGTAATCGTCCGATAGAAGAAATAC
  TTTCTCATGATAAGCTGTCAGTTCTGTGATGAAGATTCAGACTGGGAGGATGTACTGTAA
  TCCTGGCGAGGAGAATGAGAATTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002095 unedited
  NNNTTTTTCCTATGGACCGCGGGCCGCATTCTAGGATCGAGTTTTTTTTTTTTTTTTTTT
  TTTTTTTCCTTCTACAGGGATTTTATTATAAATATGTTCTTTTGGAATAAACAAATATTG
  CATTCAAGACTATGGGCAAACTTGCTCTAAAGTAACAAACGAGGAAGAAAGGAACCAGGA
  ACCATTTAAAAGTGGAGAAAAACACAGCAAAGACACCTGCATGCCACGCTCAGCGCCTTT
  CTCCCAGGGAGTAACAGAAGGTTAACAGGGAACATCACATTGCCCATGAGCCCATTCTAC
  TCAAATAAGCTTTGAGTTTGGTAATTTGCACTTGTTTTGTAAACTGAACTGCTCCTCTCC
  TCAGCCGCAAGAAGCAGATAGGAAGACAGTCTTCAGACCCCGAGCATCAGCAAGAACACT
  CTTGATTGGGTATCTGTAACTCTGTTCCAGGGCAAAACTGTTCCCTATTTGCTGGAAGTA
  ATGTCAGAGTAATCCTTCAGCACTCCAGCCAAGTGTTCGTTATGAGTCTTAAAGCGTCGC
  TTTTTCTGTGAAGCAGGCTTTTTCCTTCTCTGAATAGGGGCCACTTTCTTTGGTCCAGAT
  TCCTGCATGGAAGAAATACCCTGTCGCTTCAGATATTCTTCAATTTTCTCCTCGTCCATG
  GAATCTACAGTGACACTCCTCCACAGTTTCTGAAATTCTTCATCCACAGAAAACTGACAG
  CTCTTATCATTGAAGAAAAGTATTTTCTTCTTATCGGGACGATTTACAAATAGTATCTGG
  TCCCCCCAAGCCTTGACAGCTTTCTGGGAATTGGGCAGTGCTTCTTCTATTGTCTCTAAA
  GAATTCTCCTAACCTCGCTGGCATGCTGATCTAAGAGCCTAGTAGGGCCTTCTTATCTCT
  CACGTGTACTTGGGCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002095
• Insert Size: 1640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002095.3, NP_002086.1
• RefSeq Size: 1560 bp
• RefSeq ORF: 1560 bp
• Locus ID: 2961
• Cytogenetics: 8p12
• Domains: TFIIE_beta
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: 'The general transcription factor IIE (TFIIE) is part of the RNA polymerase II transcription initiation complex, recruiting TFIIH and being essential for promoter clearance by RNA polymerase II. TFIIE is a heterodimer (and sometimes heterotetramer) of alpha and beta subunits. The protein encoded by this gene represents the beta subunit of TFIIE. [provided by RefSeq, Jan 2017]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).