CDC34 (NM_004359) Human Tagged ORF Clone

CAT#: RG203517

CDC34 (GFP-tagged) - Human cell division cycle 34 homolog (S. cerevisiae) (CDC34)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CDC34
• Synonyms: E2-CDC34; UBC3; UBCH3; UBE2R1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203517 representing NM_004359
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCGGCCGCTAGTGCCCAGCTCGCAGAAGGCGCTGCTGCTGGAGCTCAAGGGGCTGCAGGAAGAGC
  CGGTCGAGGGATTCCGCGTGACACTGGTGGACGAGGGCGATCTATACAACTGGGAGGTGGCCATCTTCGG
  GCCCCCCAACACCTACTACGAGGGCGGCTACTTCAAGGCGCGCCTCAAGTTCCCCATCGACTACCCATAC
  TCTCCACCAGCCTTTCGGTTCCTGACCAAGATGTGGCACCCTAACATCTACGAGACGGGGGACGTGTGTA
  TCTCCATCCTCCACCCGCCGGTGGACGACCCCCAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCCCAC
  GCAGAACGTCAGGACCATTCTCCTGAGTGTGATCTCCCTCCTGAACGAGCCCAACACCTTCTCGCCCGCA
  AACGTGGACGCCTCCGTGATGTACAGGAAGTGGAAAGAGAGCAAGGGGAAGGATCGGGAGTACACAGACA
  TCATCCGGAAGCAGGTCCTGGGGACCAAGGTGGACGCGGAGCGTGACGGCGTGAAGGTGCCCACCACGCT
  GGCCGAGTACTGCGTGAAGACCAAGGCGCCGGCGCCCGACGAGGGCTCAGACCTCTTCTACGACGACTAC
  TACGAGGACGGCGAGGTGGAGGAGGAGGCCGACAGCTGCTTCGGGGACGATGAGGATGACTCTGGCACGG
  AGGAGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203517 representing NM_004359
  Red=Cloning site Green=Tags(s)
  
  MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPY
  SPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPA
  NVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDY
  YEDGEVEEEADSCFGDDEDDSGTEES
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004359
• ORF Size: 708 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004359.1, NP_004350.1
• RefSeq Size: 1462 bp
• RefSeq ORF: 711 bp
• Locus ID: 997
• Cytogenetics: 19p13.3
• Domains: UBCc
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: 'The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008]'