CDC34 (NM_004359) Human Untagged Clone

CAT#: SC103113

CDC34 (untagged)-Human cell division cycle 34 homolog (S. cerevisiae) (CDC34)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC34
• Synonyms: E2-CDC34; UBC3; UBCH3; UBE2R1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004359 edited
  CGGCACGAGGGCTCGGGGTCCCCGGGCGGCGGCGGCGGCGCAGAGGAGGAGGCAGGCGGC
  GGCCCCGGTGGCTCCCCCCCGGACGGTGCGCGGCCCGGCCCGTCTCGCGAACTCGCGGTG
  GTCGCGCGGCCCCGCGCTGCTCCGACCCCGGGCCCCTCCGCCGCCGCCATGGCTCGGCCG
  CTAGTGCCCAGCTCGCAGAAGGCGCTGCTGCTGGAGCTCAAGGGGCTGCAGGAAGAGCCG
  GTCGAGGGATTCCGCGTGACACTGGTGGACGAGGGCGATCTATACAACTGGGAGGTGGCC
  ATCTTCGGGCCCCCCAACACCTACTACGAGGGCGGCTACTTCAAGGCGCGCCTCAAGTTC
  CCCATCGACTACCCATACTCTCCACCAGCCTTTCGGTTCCTGACCAAGATGTGGCACCCT
  AACATCTACGAGACGGGGGACGTGTGTATCTCCATCCTCCACCCGCCGGTGGACGACCCC
  CAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCCCACGCAGAACGTCAGGACCATTCTC
  CTGAGTGTGATCTCCCTCCTGAACGAGCCCAACACCTTCTCGCCCGCAAACGTGGACGCC
  TCCGTGATGTACAGGAAGTGGAAAGAGAGCAAGGGGAAGGATCGGGAGTACACAGACATC
  ATCCGGAAGCAGGTCCTGGGGACCAAGGTGGACGCGGAGCGTGACGGCGTGAAGGTGCCC
  ACCACGCTGGCCGAGTACTGCGTGAAGACCAAGGCGCCGGCGCCCGACGAGGGCTCAGAC
  CTCTTCTACGACGACTACTACGAGGACGGCGAGGTGGAGGAGGAGGCCGACAGCTGCTTC
  GGGGACGATGAGGATGACTCTGGCACGGAGGAGTCCTGACACCACCAGAATAAACTTGCC
  GAGTTTACCTCACTAGGGCCGGACCCGTGGCTCCTTAGACGACAGACTACCTCACGGAGG
  TTTTGTGCTGGTCCCCGTCTCCTCTGGTTGTTTCGTTTTGGCTTTTTCTCCTTCCCCATG
  TCTGTTCTGGGTTTTCACGTGCTTCAGAGAAGAGGGGTTGCCCCACCGCCACTCACGTCA
  CTCGGGGCTCGGTGGACGGGCCCAGGGTGGGAGCGGCCGGCCCACCTGTCCCCTCGGGAG
  GGGAGCTGAGCCCGACTTCTACCGGGGTCCCCCAGCTTCCGGACTGGCCGCACCCCGGAG
  GAGCCACGGGGGCGCTGCTGGGAACGTGGGCGGGGGGCCGTTTCCTGACACTACCAGCCT
  GGGAGGCCCAGGTGTAGCGGTCCGAGGGGCCCGGTCCTGCCTGTCAGCTCCAGGTCCTGG
  AGCCACGTCCAGCACAGAGTGGACGGATTCACCGTGGCCGACTCTTTTTCCCTGCTTTGG
  TTTGTTTGAAATCTAAATAAAACTACTTTATGAGAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004359 unedited
  GGTCANCATTTGTATACGACTCCTATAGGCGGCNCGCGATTCGGCACGAGGGCTCGGGGT
  CCCCGGGCGGCGGCGGCGGCGCAGAGGAGGAGGCAGGCGGCGGCCCCGGTGGCTCCCCCC
  CGGACGGTGCGCGGCCCGGCCCGTCTCGCGAACTCGCGGTGGTCGCGCGGCCCCGCGCTG
  CTCCGACCCCGGGCCCCTCCGCCGCCGCCATGGCTCGGCCGCTAGTGCCCAGCTCGCAGA
  AGGCGCTGCTGCTGGAGCTCAAGGGGCTGCAGGAAGAGCCGGTCGAGGGATTCCGCGTGA
  CACTGGTGGACGAGGGCGATCTATACAACTGGGAGGTGGCCATCTTCGGGCCCCCCAACA
  CCTACTACGAGGGCGGCTACTTCAAGGCGCGCCTCAAGTTCCCCATCGACTACCCATACT
  CTCCACCAGCCTTTCGGTTCCTGACCAAGATGTGGCACCCTAACATCTACGAGACGGGGG
  ACGTGTGTATCTCCATCCTCCACCCGCCGGTGGACGACCCCCAGAGCGGGGAGCTGCCCT
  CAGAGAGGTGGAACCCCACGCAGAACGTCAGGACCATTCTCCTGAGTGTGATCTCCCTCC
  TGAACGAGCCCAACACCTTCTCGCCCGCAAACGTGGACGCCTCCGTGATGTACAGGAAGT
  GGAAAGAGAGCAAGGGGAAGGATCGGGAGTACACAGACATCATCCGGAAGCAGGTCCTGG
  GGACCAAGGTGGACGCCTAGCGTGACGGCGTGAAAGTGCCCACCACGCTGGCCGAGTACT
  GCGTGGAAGACCAAGGCGCCGGCGCCCCGACGAGGCTCAGACCTCTTCTACGACGACTAC
  TACNCAGACCGCNAGGTGGAAGAGAGGCCCACCGCTGCTTCGGGACGATGAGGATGACTC
  TGGCACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004359 unedited
  NTTCCTAGCTCTGNNACCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTTT
  TTTTTCTCATAAAGTAGTTTTATTTAGATTTCAAACAAACCAAAGCAGGGAAAAAGAGTC
  GGCCACGGTGAATCCGTCCACTCTGTGCTGGACGTGGCTCCAGGACCTGGAGCTGACAGG
  CAGGACCGGGCCCCTCGGACCGCTACACCTGGGCCTCCCAGGCTGGTAGTGTCAGGAAAC
  GGCCCCCCGCCCACGTTCCCAGCAGCGCCCCCGTGGCTCCTCCGGGGTGCGGCCAGTCCG
  GAAGCTGGGGGACCCCGGTAGAAGTCGGGCTCAGCTCCCCTCCCGAGGGGACAGGTGGGC
  CGGCCGCTCCCACCCTGGGCCCGTCCACCGAGCCCCGAGTGACGTGAGTGGCGGTGGGGC
  AACCCCTCTTCTCTGAAGCACGTGAAAACCCAGAACAGACATGGGGAAGGAGAAAAAGCC
  AAAACGAAACAACCAGAGGAGACGGGGACCAGCACAAAACCTCCGTGAGGTAGTCTGTCG
  TCTAAGGAGCCACGGGTCCGGCCCTAGTGAGGTAAACTCGGCAAGTTTATTCTGGTGGTG
  TCAGGACTCCTCCGTGCCAGAGTCATCCTCATCGTCCCCGAAGCAGCTGTCGGCCTCCTC
  CTCCACCTNGCCGTCCTCGTAGTAGTCGTCGTAGAAGAGGTCTGAGCCCCTCTCGGGCGC
  CCGCGCCTTGGTCTCACGCAGTACTCGGCCAGCGTGGTGGGCACCTTCACGCCGTCACGC
  TTCGCGTCCACCTTGGTCCCCAGGACCTGCTTCCGGATGAGTCTGTGTACTCCCGATCCT
  TTCCTTGCTCTTTTTCCACTCCTGTACTAACGGAGGCGTCCACTTTTCCGGCAAAAAGTG
  TTTGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004359
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004359.1, NP_004350.1
• RefSeq Size: 1462 bp
• RefSeq ORF: 711 bp
• Locus ID: 997
• Cytogenetics: 19p13.3
• Domains: UBCc
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: 'The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008]'