OXCT1 (NM_000436) Human Tagged ORF Clone

CAT#: RG203764

OXCT1 (GFP-tagged) - Human 3-oxoacid CoA transferase 1 (OXCT1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: OXCT1
• Synonyms: OXCT; SCOT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203764 representing NM_000436
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTCTCAAACTCCTCTCCTCCGGGCTTCGGCTCTGCGCCTCTGCCCGCGGATCTGGGGCAACCT
  GGTACAAGGGATGTGTTTGTTCCTTTTCCACCAGTGCTCATCGCCATACCAAGTTTTATACAGATCCAGT
  AGAAGCTGTAAAAGACATCCCTGATGGTGCCACGGTTTTGGTTGGTGGTTTTGGGCTATGTGGAATTCCA
  GAGAATCTTATAGATGCTTTACTGAAAACTGGAGTAAAAGGACTAACTGCAGTCAGCAACAATGCAGGGG
  TTGACAATTTTGGTTTGGGGCTTTTGCTTCGGTCCAAGCAGATAAAACGCATGGTCTCTTCATATGTGGG
  AGAAAATGCAGAATTTGAACGACAGTACTTATCTGGTGAATTAGAAGTGGAGCTGACACCACAGGGCACA
  CTTGCAGAGAGGATCCGTGCAGGCGGGGCTGGAGTTCCTGCATTTTACACCCCAACAGGGTATGGGACCC
  TGGTACAAGAAGGAGGATCGCCCATCAAATACAACAAAGATGGCAGTGTTGCCATTGCCAGTAAGCCAAG
  AGAGGTGAGGGAGTTCAATGGTCAGCACTTTATTTTGGAGGAAGCAATTACAGGGGATTTTGCTTTGGTG
  AAAGCCTGGAAGGCGGACCGAGCAGGAAACGTGATTTTCAGGAAAAGTGCAAGGAATTTCAACTTGCCAA
  TGTGCAAAGCTGCAGAAACCACAGTGGTAGAGGTTGAAGAAATTGTGGATATTGGAGCATTTGCTCCAGA
  AGACATCCATATTCCTCAGATTTATGTACATCGCCTTATAAAGGGAGAAAAATATGAGAAAAGAATTGAG
  CGTTTATCAATCCGGAAAGAGGGAGATGGGGAAGCCAAATCTGCTAAACCTGGAGATGACGTAAGGGAAC
  GAATCATCAAGAGGGCCGCTCTTGAGTTTGAGGATGGCATGTATGCTAATTTGGGCATAGGAATCCCTCT
  CCTGGCCAGCAATTTTATCAGCCCAAATATAACTGTTCATCTTCAAAGTGAAAATGGAGTTCTGGGTTTG
  GGTCCATATCCACGACAACATGAAGCTGATGCAGATCTCATCAATGCAGGCAAGGAAACAGTTACTATTC
  TTCCAGGAGCCTCTTTTTTCTCCAGCGATGAATCATTTGCAATGATTAGAGGTGGACACGTCGATCTGAC
  AATGCTAGGAGCGATGCAGGTTTCCAAATATGGTGACCTGGCTAACTGGATGATACCTGGGAAGATGGTG
  AAAGGAATGGGAGGTGCTATGGATTTAGTGTCCAGTGCGAAAACCAAAGTGGTGGTCACCATGGAGCATT
  CTGCAAAGGGAAATGCACATAAAATCATGGAGAAATGTACATTACCATTGACTGGAAAGCAATGTGTCAA
  CCGCATTATTACTGAAAAGGCTGTGTTTGATGTGGACAAGAAGAAAGGGTTGACTCTGATTGAGCTCTGG
  GAAGGCCTGACAGTGGATGACGTACAGAAGAGTACTGGGTGTGATTTTGCAGTTTCACCAAAACTCATGC
  CAATGCAGCAGATCGCAAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203764 representing NM_000436
  Red=Cloning site Green=Tags(s)
  
  MAALKLLSSGLRLCASARGSGATWYKGCVCSFSTSAHRHTKFYTDPVEAVKDIPDGATVLVGGFGLCGIP
  ENLIDALLKTGVKGLTAVSNNAGVDNFGLGLLLRSKQIKRMVSSYVGENAEFERQYLSGELEVELTPQGT
  LAERIRAGGAGVPAFYTPTGYGTLVQEGGSPIKYNKDGSVAIASKPREVREFNGQHFILEEAITGDFALV
  KAWKADRAGNVIFRKSARNFNLPMCKAAETTVVEVEEIVDIGAFAPEDIHIPQIYVHRLIKGEKYEKRIE
  RLSIRKEGDGEAKSAKPGDDVRERIIKRAALEFEDGMYANLGIGIPLLASNFISPNITVHLQSENGVLGL
  GPYPRQHEADADLINAGKETVTILPGASFFSSDESFAMIRGGHVDLTMLGAMQVSKYGDLANWMIPGKMV
  KGMGGAMDLVSSAKTKVVVTMEHSAKGNAHKIMEKCTLPLTGKQCVNRIITEKAVFDVDKKKGLTLIELW
  EGLTVDDVQKSTGCDFAVSPKLMPMQQIAN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000436
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000436.3, NP_000427.1
• RefSeq Size: 3572 bp
• RefSeq ORF: 1563 bp
• Locus ID: 5019
• Cytogenetics: 5p13.1
• Domains: CoA_trans
• Protein Pathways: Butanoate metabolism, Synthesis and degradation of ketone bodies, Valine, leucine and isoleucine degradation
• Gene Summary: 'This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency. [provided by RefSeq, Jul 2008]'