OXCT1 (NM_000436) Human Untagged Clone

CAT#: SC119890

OXCT1 (untagged)-Human 3-oxoacid CoA transferase 1 (OXCT1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OXCT1
• Synonyms: OXCT; SCOT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119890 sequence for NM_000436 edited (data generated by NextGen Sequencing)
  ATGGCGGCTCTCAAACTCCTCTCCTCCGGGCTTCGGCTCTGCGCCTCTGCCCGCGGATCT
  GGGGCAACCTGGTACAAGGGATGTGTTTGTTCCTTTTCCACCAGTGCTCATCGCCATACC
  AAGTTTTATACAGATCCAGTAGAAGCTGTAAAAGACATCCCTGATGGTGCCACGGTTTTG
  GTTGGTGGTTTTGGGCTATGTGGAATTCCAGAGAATCTTATAGATGCTTTACTGAAAACT
  GGAGTAAAAGGACTAACTGCAGTCAGCAACAATGCAGGGGTTGACAATTTTGGTTTGGGG
  CTTTTGCTTCGGTCCAAGCAGATAAAACGCATGGTCTCTTCATATGTGGGAGAAAATGCA
  GAATTTGAACGACAGTACTTATCTGGTGAATTAGAAGTGGAGCTGACACCACAGGGCACA
  CTTGCAGAGAGGATCCGTGCAGGCGGGGCTGGAGTTCCTGCATTTTACACCCCAACAGGG
  TATGGGACCCTGGTACAAGAAGGAGGATCGCCCATCAAATACAACAAAGATGGCAGTGTT
  GCCATTGCCAGTAAGCCAAGAGAGGTGAGGGAGTTCAATGGTCAGCACTTTATTTTGGAG
  GAAGCAATTACAGGGGATTTTGCTTTGGTGAAAGCCTGGAAGGCGGACCGAGCAGGAAAC
  GTGATTTTCAGGAAAAGTGCAAGGAATTTCAACTTGCCAATGTGCAAAGCTGCAGAAACC
  ACAGTGGTAGAGGTTGAAGAAATTGTGGATATTGGAGCATTTGCTCCAGAAGACATCCAT
  ATTCCTCAGATTTATGTACATCGCCTTATAAAGGGAGAAAAATATGAGAAAAGAATTGAG
  CGTTTATCAATCCGGAAAGAGGGAGATGGGGAAGCCAAATCTGCTAAACCTGGAGATGAC
  GTAAGGGAACGAATCATCAAGAGGGCCGCTCTTGAGTTTGAGGATGGCATGTATGCTAAT
  TTGGGCATAGGAATCCCTCTCCTGGCCAGCAATTTTATCAGCCCAAATATAACTGTTCAT
  CTTCAAAGTGAAAATGGAGTTCTGGGTTTGGGTCCATATCCACGACAACATGAAGCTGAT
  GCAGATCTCATCAATGCAGGCAAGGAAACAGTTACTATTCTTCCAGGAGCCTCTTTTTTC
  TCCAGCGATGAATCATTTGCAATGATTAGAGGTGGACACGTCGATCTGACAATGCTAGGA
  GCGATGCAGGTTTCCAAATATGGTGACCTGGCTAACTGGATGATACCTGGGAAGATGGTG
  AAAGGAATGGGAGGTGCTATGGATTTAGTGTCCAGTGCGAAAACCAAAGTGGTGGTCACC
  ATGGAGCATTCTGCAAAGGGAAATGCACATAAAATCATGGAGAAATGTACATTACCATTG
  ACTGGAAAGCAATGTGTCAACCGCATTATTACTGAAAAGGCTGTGTTTGATGTGGACAAG
  AAGAAAGGGTTGACTCTGATTGAGCTCTGGGAAGGCCTGACAGTGGATGACGTACAGAAG
  AGTACTGGGTGTGATTTTGCAGTTTCACCAAAACTCATGCCAATGCAGCAGATCGCAAAT
  TGA
  
  Clone variation with respect to NM_000436.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000436 unedited
  TGTATACGACTCACTATAGGCGGCNCGCGAATTCGCACGAGGCTCAAACGCGCGCTCCAA
  CCCGCAGCCTCCTCCTGCCTCACCGCCCGAAGAGGCGGCTCTCAAACTCCTCTCCTCCGG
  GCTTCGGCTCTGCGCCTCTGCCCGCGGATCTGGGGCAACCTGGTACAAGGGATGTGTTTG
  TTCCTTTTCCACCAGTGCTCATCGCCATACCAAGTTTTATACAGATCCAGTAGAAGCTGT
  AAAAGACATCCCTGATGGTGCCACGGTTTTGGTTGGTGGTTTTGGGCTATGTGGAATTCC
  AGAGAATCTTATAGATGCTTTACTGAAAACTGGAGTAAAAGGACTAACTGCAGTCAGCAA
  CAATGCAGGGGTTGACAATTTTGGTTTGGGGCTTTTGCTTCGGTCCAAGCAGATAAAACG
  CATGGTCTCTTCATATGTGGGAGAAAATGCAGAATTTGAACGACAGTACTTATCTGGTGA
  ATTAGAAGTGGAGCTGACACCACAGGGCACACTTGCAGAGAGGATCCGTGCAGGCGGNGC
  TGGAGTTCCTGCATTNTACACCCNCAACAGGGTATGGGACCCTGGTACAAGAGGGGAGAT
  CGCCCATCAAATACAACAAAGATGGCAGTGTTGCCATTGCCAGTAAGCCAAGAGAGGTGA
  AGGGAGTTCAATGGTCANCACTTTATTTTGGAGGAAGCAATACAGGNGATTTTGCTTTTG
  TGAAAGCCTGAAGGGCGACCGACAGNAAACGTGATTTTCAGGAAAGTGCAGGATTTCACT
  TGCCATGTGCAAGCTGAAAACACAGGGNTNAAGGTGAAAAATTGTGATATGGGACTTTGC
  TCAGAGACTCCATATCCTAAATTTAGGCCATGCCTTATAAGGAGAAAATGAAAGGAATTG
  GCGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000436 unedited
  GACCGCGGCACGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTTACATCACTTTAGAA
  TATTTATTGTATTCCTTAATGCATTTCTTAACATGTATAGCACTCTTTAATCAAGAATAT
  AAAGTCATCTACTTAGAATCACATTATCTTAAAGATGCATACTGGAATGATAAGTTTGAA
  GATGTAACTATCAACAATTCTTTTCAAAATCATATCAATATATTACTCTCATGGAACTTG
  CACATTCTAAGAAGGGTCATTTTTTCCCCCCAGTACTGGGAAGGTATGCATTTAACCATG
  TGGTCAGCCAGAAAGGCTGTTTTATATATGGTGTGTGTTACTCATAAAAAGCCTGTCCTT
  TCTCTAATATTGTACATTACACAATTGTAGCTACACCTGAAAAGAAGTGAGTTTCAATGA
  CCATCATCACCTAAAAACATGAATAAGTTTGTATATTTAGGACCAGAGGAATGATATATC
  GTACTGTACTATGCCTACCTCTGTCCAGAGGCCAGCTGTCTCACCTGTTCTTCAGGGTGC
  CTAGACTTTTTACCCATCTATTATCCGGGATGCTTCTGAAGTCATTCCCATGGGCCCCTG
  TCAGGTATTGAGAATAAATACCTCTAAGGAAGTTTTTTTCAAACCTAATCTACCCACCAT
  GTTTATTCCCTGACACTAAAAGTTCACTGACTCTTCAAATCCTAGACTTGTCCAACATTA
  TTTTTCCCTAGTTGGATATGGCTGAATTAAAAGGCACTTCGTTAAAGGGAAGTAGCCCTG
  GGCAGAAGATAAACCGTTGTTCTGTAAAAGTGTCATTCAATATTTAAAATCTNCTCTGAA
  CCANNAACAGACCNNTGAAGTNAAAACACCTTTTATTTTTTAACTCTGCTGGCATTGCTA
  CTAAAGATTCATGGGAAATNATCCCCACAGCCTTTCTTGGCTTGAAAGGAGGTACAGTCA
  AGTACCCCAAGGTCTCATAACAGAAGTATCCTCAGGACCCTTGAGGATCAAGAATACTGG
  GCTGTTTAAATGAGAAACTGNAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000436
• Insert Size: 3330 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000436.2, NP_000427.1
• RefSeq Size: 3385 bp
• RefSeq ORF: 1563 bp
• Locus ID: 5019
• Cytogenetics: 5p13.1
• Domains: CoA_trans
• Protein Pathways: Butanoate metabolism, Synthesis and degradation of ketone bodies, Valine, leucine and isoleucine degradation
• Gene Summary: 'This gene encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency. [provided by RefSeq, Jul 2008]'