NC2 alpha (DRAP1) (NM_006442) Human Tagged ORF Clone

CAT#: RG204031

DRAP1 (GFP-tagged) - Human DR1-associated protein 1 (negative cofactor 2 alpha) (DRAP1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DRAP1
• Synonyms: NC2-alpha
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204031 representing NM_006442
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAGCAAGAAGAAGAAGTACAACGCGCGGTTCCCGCCGGCGCGGATCAAGAAGATCATGCAGACGG
  ACGAAGAGATTGGGAAGGTGGCGGCGGCGGTGCCTGTCATCATCTCCCGGGCGCTCGAGCTCTTCCTAGA
  GTCGCTGTTGAAGAAGGCCTGCCAGGTGACCCAGTCGCGGAACGCGAAGACCATGACCACATCCCACCTG
  AAGCAGTGCATCGAGCTGGAGCAGCAGTTTGACTTCTTGAAGGACCTGGTGGCATCTGTTCCCGACATGC
  AGGGGGACGGGGAAGACAACCACATGGATGGGGACAAGGGCGCCCGCAGGGGCCGGAAGCCAGGCAGCGG
  CGGCCGGAAGAACGGTGGGATGGGAACGAAAAGCAAGGACAAGAAGCTGTCCGGGACAGACTCGGAGCAG
  GAGGATGAATCTGAGGACACAGATACTGATGGGGAAGAGGAGACATCACAACCCCCACCCCAGGCCAGCC
  ACCCCTCTGCCCACTTTCAGAGCCCCCCGACACCCTTCCTGCCCTTCGCCTCTACTCTGCCTTTGCCCCC
  AGCGCCCCCGGGCCCCTCAGCACCTGATGAAGAGGACGAAGAAGATTACGACTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204031 representing NM_006442
  Red=Cloning site Green=Tags(s)
  
  MPSKKKKYNARFPPARIKKIMQTDEEIGKVAAAVPVIISRALELFLESLLKKACQVTQSRNAKTMTTSHL
  KQCIELEQQFDFLKDLVASVPDMQGDGEDNHMDGDKGARRGRKPGSGGRKNGGMGTKSKDKKLSGTDSEQ
  EDESEDTDTDGEEETSQPPPQASHPSAHFQSPPTPFLPFASTLPLPPAPPGPSAPDEEDEEDYDS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006442
• ORF Size: 615 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006442.2, NP_006433.2
• RefSeq Size: 988
• RefSeq ORF: 618
• Locus ID: 10589
• Protein Families: Transcription Factors
• Gene Summary: Transcriptional repression is a general mechanism for regulating transcriptional initiation in organisms ranging from yeast to humans. Accurate initiation of transcription from eukaryotic protein-encoding genes requires the assembly of a large multiprotein complex consisting of RNA polymerase II and general transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a repressor that interacts with the TATA-binding protein (TBP) of TFIID and prevents the formation of an active transcription complex by precluding the entry of TFIIA and/or TFIIB into the preinitiation complex. The protein encoded by this gene is a corepressor of transcription that interacts with DR1 to enhance DR1-mediated repression. The interaction between this corepressor and DR1 is required for corepressor function and appears to stabilize the TBP-DR1-DNA complex. [provided by RefSeq, Jul 2008]