GMP Synthase (GMPS) (NM_003875) Human Tagged ORF Clone

CAT#: RG204267

GMPS (GFP-tagged) - Human guanine monphosphate synthetase (GMPS)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GMPS
• Synonyms: GATD7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204267 representing NM_003875
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGTGCAACGGAGACTCCAAGCTGGAGAATGCTGGAGGAGACCTTAAGGATGGCCACCACCACT
  ATGAAGGAGCTGTTGTCATTCTGGATGCTGGTGCTCAGTACGGGAAAGTCATAGACCGAAGAGTGAGGGA
  ACTGTTCGTGCAGTCTGAAATTTTCCCCTTGGAAACACCAGCATTTGCTATAAAGGAACAAGGATTCCGT
  GCTATTATCATCTCTGGAGGACCTAATTCTGTGTATGCTGAAGATGCTCCCTGGTTTGATCCAGCAATAT
  TCACTATTGGCAAGCCTGTTCTTGGAATTTGCTATGGTATGCAGATGATGAATAAGGTATTTGGAGGTAC
  TGTGCACAAAAAAAGTGTCAGAGAAGATGGAGTTTTCAACATTAGTGTGGATAATACATGTTCATTATTC
  AGGGGCCTTCAGAAGGAAGAAGTTGTTTTGCTTACACATGGAGATAGTGTAGACAAAGTAGCTGATGGAT
  TCAAGGTTGTGGCACGTTCTGGAAACATAGTAGCAGGCATAGCAAATGAATCTAAAAAGTTATATGGAGC
  ACAGTTCCACCCTGAAGTTGGCCTTACAGAAAATGGAAAAGTAATACTGAAGAATTTCCTTTATGATATA
  GCTGGATGCAGTGGAACCTTCACCGTGCAGAACAGAGAACTTGAGTGTATTCGAGAGATCAAAGAGAGAG
  TAGGCACGTCAAAAGTTTTGGTTTTACTCAGTGGTGGAGTAGACTCAACAGTTTGTACAGCTTTGCTAAA
  TCGTGCTTTGAACCAAGAACAAGTCATTGCTGTGCACATTGATAATGGCTTTATGAGAAAACGAGAAAGC
  CAGTCTGTTGAAGAGGCCCTCAAAAAGCTTGGAATTCAGGTCAAAGTGATAAATGCTGCTCATTCTTTCT
  ACAATGGAACAACAACCCTACCAATATCAGATGAAGATAGAACCCCACGGAAAAGAATTAGCAAAACGTT
  AAATATGACCACAAGTCCTGAAGAGAAAAGAAAAATCATTGGGGATACTTTTGTTAAGATTGCCAATGAA
  GTAATTGGAGAAATGAACTTGAAACCAGAGGAGGTTTTCCTTGCCCAAGGTACTTTACGGCCTGATCTAA
  TTGAAAGTGCATCCCTTGTTGCAAGTGGCAAAGCTGAACTCATCAAAACCCATCACAATGACACAGAGCT
  CATCAGAAAGTTGAGAGAGGAGGGAAAAGTAATAGAACCTCTGAAAGATTTTCATAAAGATGAAGTGAGA
  ATTTTGGGCAGAGAACTTGGACTTCCAGAAGAGTTAGTTTCCAGGCATCCATTTCCAGGTCCTGGCCTGG
  CAATCAGAGTAATATGTGCTGAAGAACCTTATATTTGTAAGGACTTTCCTGAAACCAACAATATTTTGAA
  AATAGTAGCTGATTTTTCTGCAAGTGTTAAAAAGCCACATACCCTATTACAGAGAGTCAAAGCCTGCACA
  ACAGAAGAGGATCAGGAGAAGCTGATGCAAATTACCAGTCTGCATTCACTGAATGCCTTCTTGCTGCCAA
  TTAAAACTGTAGGTGTGCAGGGTGACTGTCGTTCCTACAGTTACGTGTGTGGAATCTCCAGTAAAGATGA
  ACCTGACTGGGAATCACTTATTTTTCTGGCTAGGCTTATACCTCGCATGTGTCACAACGTTAACAGAGTT
  GTTTATATATTTGGCCCACCAGTTAAAGAACCTCCTACAGATGTTACTCCCACTTTCTTGACAACAGGGG
  TGCTCAGTACTTTACGCCAAGCTGATTTTGAGGCCCATAACATTCTCAGGGAGTCTGGGTATGCTGGGAA
  AATCAGCCAGATGCCGGTGATTTTGACACCATTACATTTTGATCGGGACCCACTTCAAAAGCAGCCTTCA
  TGCCAGAGATCTGTGGTTATTCGAACCTTTATTACTAGTGACTTCATGACTGGTATACCTGCAACACCTG
  GCAATGAGATCCCTGTAGAGGTGGTATTAAAGATGGTCACTGAGATTAAGAAGATTCCTGGTATTTCTCG
  AATTATGTATGACTTAACATCAAAGCCCCCAGGAACTACTGAGTGGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204267 representing NM_003875
  Red=Cloning site Green=Tags(s)
  
  MALCNGDSKLENAGGDLKDGHHHYEGAVVILDAGAQYGKVIDRRVRELFVQSEIFPLETPAFAIKEQGFR
  AIIISGGPNSVYAEDAPWFDPAIFTIGKPVLGICYGMQMMNKVFGGTVHKKSVREDGVFNISVDNTCSLF
  RGLQKEEVVLLTHGDSVDKVADGFKVVARSGNIVAGIANESKKLYGAQFHPEVGLTENGKVILKNFLYDI
  AGCSGTFTVQNRELECIREIKERVGTSKVLVLLSGGVDSTVCTALLNRALNQEQVIAVHIDNGFMRKRES
  QSVEEALKKLGIQVKVINAAHSFYNGTTTLPISDEDRTPRKRISKTLNMTTSPEEKRKIIGDTFVKIANE
  VIGEMNLKPEEVFLAQGTLRPDLIESASLVASGKAELIKTHHNDTELIRKLREEGKVIEPLKDFHKDEVR
  ILGRELGLPEELVSRHPFPGPGLAIRVICAEEPYICKDFPETNNILKIVADFSASVKKPHTLLQRVKACT
  TEEDQEKLMQITSLHSLNAFLLPIKTVGVQGDCRSYSYVCGISSKDEPDWESLIFLARLIPRMCHNVNRV
  VYIFGPPVKEPPTDVTPTFLTTGVLSTLRQADFEAHNILRESGYAGKISQMPVILTPLHFDRDPLQKQPS
  CQRSVVIRTFITSDFMTGIPATPGNEIPVEVVLKMVTEIKKIPGISRIMYDLTSKPPGTTEWE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003875
• ORF Size: 2079 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003875.2, NP_003866.1
• RefSeq Size: 2457
• RefSeq ORF: 2082
• Locus ID: 8833
• Domains: GATase, GMP_synt_C
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism
• Gene Summary: In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP. [provided by RefSeq, Jul 2008]