CHMP5 (NM_016410) Human Tagged ORF Clone

CAT#: RG204779

CHMP5 (GFP-tagged) - Human chromatin modifying protein 5 (CHMP5), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CHMP5
• Synonyms: C9orf83; CGI-34; HSPC177; PNAS-2; SNF7DC2; Vps60
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204779 representing NM_016410
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCGACTCTTCGGGAAAGCGAAACCCAAGGCTCCGCCGCCCAGCCTGACTGGCTGCATTGGCACGG
  TGGACAGTAGAGCAGAATCCATTGACAAGAAGATTTCTCGATTGGATGCTGAGCTAGTGAAGTATAAGGA
  TCAGATCAAGAAGATGAGAGAGGGTCCTGCAAAGAATATGGTCAAGCAGAAAGCCTTGCGAGTTTTAAAG
  CAAAAGAGGATGTATGAGCAGCAGCGGGACAATCTTGCCCAACAGTCATTCAACATGGAACAAGCCAATT
  ATACCATCCAGTCTTTGAAGGACACCAAGACCACGGTTGATGCTATGAAACTGGGAGTAAAGGAAATGAA
  GAAGGCATACAAGCAAGTGAAGATCGACCAGATTGAGGATTTACAAGACCAGCTAGAGGATATGATGGAA
  GATGCAAATGAAATCCAAGAAGCACTGAGTCGCAGTTATGGCACCCCAGAACTGGATGAAGATGATTTAG
  AAGCAGAGTTGGATGCACTAGGTGATGAGCTTCTGGCTGATGAAGACAGTTCTTATTTGGATGAGGCAGC
  ATCTGCACCTGCAATTCCAGAAGGTGTTCCCACTGATACAAAAAACAAGGATGGAGTTCTGGTGGATGAA
  TTTGGATTGCCACAGATCCCTGCTTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204779 representing NM_016410
  Red=Cloning site Green=Tags(s)
  
  MNRLFGKAKPKAPPPSLTGCIGTVDSRAESIDKKISRLDAELVKYKDQIKKMREGPAKNMVKQKALRVLK
  QKRMYEQQRDNLAQQSFNMEQANYTIQSLKDTKTTVDAMKLGVKEMKKAYKQVKIDQIEDLQDQLEDMME
  DANEIQEALSRSYGTPELDEDDLEAELDALGDELLADEDSSYLDEAASAPAIPEGVPTDTKNKDGVLVDE
  FGLPQIPAS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016410
• ORF Size: 657 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016410.2, NP_057494.2
• RefSeq Size: 1434
• RefSeq ORF: 660
• Locus ID: 51510
• Domains: DUF279
• Protein Pathways: Endocytosis
• Gene Summary: CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]). [supplied by OMIM, Mar 2008]