CBLB (NM_170662) Human Tagged ORF Clone

CAT#: RG206047

CBLB (GFP-tagged) - Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CBLB
• Synonyms: Cbl-b; Nbla00127; RNF56
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206047 representing NM_170662
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAACTCAATGAATGGCAGAAACCCTGGTGGTCGAGGAGGAAATCCCCGAAAAGGTCGAATTTTGG
  GTATTATTGATGCTATTCAGGATGCAGTTGGACCCCCTAAGCAAGCTGCCGCAGATCGCAGGACCGTGGA
  GAAGACTTGGAAGCTCATGGACAAAGTGGTAAGACTGTGCCAAAATCCCAAACTTCAGTTGAAAAATAGC
  CCACCATATATACTTGATATTTTGCCTGATACATATCAGCATTTACGACTTATATTGAGTAAATATGATG
  ACAACCAGAAACTTGCCCAACTCAGTGAGAATGAGTACTTTAAAATCTACATTGATAGCCTTATGAAAAA
  GTCAAAACGGGCAATAAGACTCTTTAAAGAAGGCAAGGAGAGAATGTATGAAGAACAGTCACAGGACAGA
  CGAAATCTCACAAAACTGTCCCTTATCTTCAGTCACATGCTGGCAGAAATCAAAGCAATCTTTCCCAATG
  GTCAATTCCAGGGAGATAACTTTCGTATCACAAAAGCAGATGCTGCTGAATTCTGGAGAAAGTTTTTTGG
  AGACAAAACTATCGTACCATGGAAAGTATTCAGACAGTGCCTTCATGAGGTCCACCAGATTAGCTCTGGC
  CTGGAAGCAATGGCTCTAAAATCAACAATTGATTTAACTTGCAATGATTACATTTCAGTTTTTGAATTTG
  ATATTTTTACCAGGCTGTTTCAGCCTTGGGGCTCTATTTTGCGGAATTGGAATTTCTTAGCTGTGACACA
  TCCAGGTTACATGGCATTTCTCACATATGATGAAGTTAAAGCACGACTACAGAAATATAGCACCAAACCC
  GGAAGCTATATTTTCCGGTTAAGTTGCACTCGATTGGGACAGTGGGCCATTGGCTATGTGACTGGGGATG
  GGAATATCTTACAGACCATACCTCATAACAAGCCCTTATTTCAAGCCCTGATTGATGGCAGCAGGGAAGG
  ATTTTATCTTTATCCTGATGGGAGGAGTTATAATCCTGATTTAACTGGATTATGTGAACCTACACCTCAT
  GACCATATAAAAGTTACACAGGAACAATATGAATTATATTGTGAAATGGGCTCCACTTTTCAGCTCTGTA
  AGATTTGTGCAGAGAATGACAAAGATGTCAAGATTGAGCCTTGTGGGCATTTGATGTGCACCTCTTGCCT
  TACGGCATGGCAGGAGTCGGATGGTCAGGGCTGCCCTTTCTGTCGTTGTGAAATAAAAGGAACTGAGCCC
  ATAATCGTGGATCCCTTTGATCCAAGAGATGAAGGCTCCAGGTGTTGCAGCATCATTGACCCCTTTGGCA
  TGCCGATGCTCGACTTGGACGACGATGATGATCGTGAGGAGTCCTTGATGATGAATCGGTTGGCAAACGT
  CCGAAAGTGCACTGACAGGCAGAACTCACCAGTCACATCACCAGGATCCTCTCCCCTTGCCCAGAGAAGA
  AAGCCACAGCCTGACCCACTCCAGATCCCACATCTAAGCCTGCCACCCGTGCCTCCTCGCCTGGATCTAA
  TTCAGAAAGGCATAGTTAGATCTCCCTGTGGCAGCCCAACGGGTTCACCAAAGTCTTCTCCTTGCATGGT
  GAGAAAACAAGATAAACCACTCCCAGCACCACCTCCTCCCTTAAGAGATCCTCCTCCACCGCCACCTGAA
  AGACCTCCACCAATCCCACCAGACAATAGACTGAGTAGACACATCCATCATGTGGAAAGCGTGCCTTCCA
  AAGACCCGCCAATGCCTCTTGAAGCATGGTGCCCTCGGGATGTGTTTGGGACTAATCAGCTTGTGGGATG
  TCGACTCCTAGGGGAGGGCTCTCCAAAACCTGGAATCACAGCGAGTTCAAATGTCAATGGAAGGCACAGT
  AGAGTGGGCTCTGACCCAGTGCTTATGCGGAAACACAGACGCCATGATTTGCCTTTAGAAGGAGCTAAGG
  TCTTTTCCAATGGTCACCTTGGAAGTGAAGAATATGATGTTCCTCCCCGGCTTTCTCCTCCTCCTCCAGT
  TACCACCCTCCTCCCTAGCATAAAGTGTACTGGTCCGTTAGCAAATTCTCTTTCAGAGAAAACAAGAGAC
  CCAGTAGAGGAAGATGATGATGAATACAAGATTCCTTCATCCCACCCTGTTTCCCTGAATTCACAACCAT
  CTCATTGTCATAATGTAAAACCTCCTGTTCGGTCTTGTGATAATGGTCACTGTATGCTGAATGGAACACA
  TGGTCCATCTTCAGAGAAGAAATCAAACATCCCTGACTTAAGCATATATTTAAAGGGAGATGTTTTTGAT
  TCAGCCTCTGATCCCGTGCCATTACCACCTGCCAGGCCTCCAACTCGGGACAATCCAAAGCATGGTTCTT
  CACTCAACAGGACGCCCTCTGATTATGATCTTCTCATCCCTCCATTAGGTGAAGATGCTTTTGATGCCCT
  CCCTCCATCTCTCCCACCTCCCCCACCTCCTGCAAGGCATAGTCTCATTGAACATTCAAAACCTCCTGGC
  TCCAGTAGCCGGCCATCCTCAGGACAGGATCTTTTTCTTCTTCCTTCAGATCCCTTTGTTGATCTAGCAA
  GTGGCCAAGTTCCTTTGCCTCCCGCTAGAAGGTTACCAGGTGAAAATGTCAAAACTAACAGAACATCACA
  GGACTATGATCAGCTTCCTTCATGTTCAGATGGTTCACAGGCACCAGCCAGACCCCCTAAACCACGACCG
  CGCAGGACTGCACCAGAAATTCACCACAGAAAACCCCATGGGCCTGAGGCGGCATTGGAAAATGTCGATG
  CAAAAATTGCAAAACTCATGGGAGAGGGTTATGCCTTTGAAGAGGTGAAGAGAGCCTTAGAGATAGCCCA
  GAATAATGTCGAAGTTGCCCGGAGCATCCTCCGAGAATTTGCCTTCCCTCCTCCAGTATCCCCACGTCTA
  AATCTA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206047 representing NM_170662
  Red=Cloning site Green=Tags(s)
  
  MANSMNGRNPGGRGGNPRKGRILGIIDAIQDAVGPPKQAAADRRTVEKTWKLMDKVVRLCQNPKLQLKNS
  PPYILDILPDTYQHLRLILSKYDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQDR
  RNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEVHQISSG
  LEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLAVTHPGYMAFLTYDEVKARLQKYSTKP
  GSYIFRLSCTRLGQWAIGYVTGDGNILQTIPHNKPLFQALIDGSREGFYLYPDGRSYNPDLTGLCEPTPH
  DHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESDGQGCPFCRCEIKGTEP
  IIVDPFDPRDEGSRCCSIIDPFGMPMLDLDDDDDREESLMMNRLANVRKCTDRQNSPVTSPGSSPLAQRR
  KPQPDPLQIPHLSLPPVPPRLDLIQKGIVRSPCGSPTGSPKSSPCMVRKQDKPLPAPPPPLRDPPPPPPE
  RPPPIPPDNRLSRHIHHVESVPSKDPPMPLEAWCPRDVFGTNQLVGCRLLGEGSPKPGITASSNVNGRHS
  RVGSDPVLMRKHRRHDLPLEGAKVFSNGHLGSEEYDVPPRLSPPPPVTTLLPSIKCTGPLANSLSEKTRD
  PVEEDDDEYKIPSSHPVSLNSQPSHCHNVKPPVRSCDNGHCMLNGTHGPSSEKKSNIPDLSIYLKGDVFD
  SASDPVPLPPARPPTRDNPKHGSSLNRTPSDYDLLIPPLGEDAFDALPPSLPPPPPPARHSLIEHSKPPG
  SSSRPSSGQDLFLLPSDPFVDLASGQVPLPPARRLPGENVKTNRTSQDYDQLPSCSDGSQAPARPPKPRP
  RRTAPEIHHRKPHGPEAALENVDAKIAKLMGEGYAFEEVKRALEIAQNNVEVARSILREFAFPPPVSPRL
  NL
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_170662
• ORF Size: 2946 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170662.3, NP_733762.2
• RefSeq Size: 3976 bp
• RefSeq ORF: 2949 bp
• Locus ID: 868
• Cytogenetics: 3q13.11
• Protein Families: Druggable Genome
• Protein Pathways: Chronic myeloid leukemia, Endocytosis, ErbB signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, Pathways in cancer, T cell receptor signaling pathway, Ubiquitin mediated proteolysis
• Gene Summary: 'This gene encodes an E3 ubiquitin-protein ligase which promotes proteosome-mediated protein degradation by transferring ubiquitin from an E2 ubiquitin-conjugating enzyme to a substrate. The encoded protein is involved in the regulation of immune response by limiting T-cell receptor, B-cell receptor, and high affinity immunoglobulin epsilon receptor activation. Studies in mouse suggest that this gene is involved in antifungal host defense and that its inhibition leads to increased fungal killing. Manipulation of this gene may be beneficial in implementing immunotherapies for a variety of conditions, including cancer, autoimmune diseases, allergies, and infections. [provided by RefSeq, Sep 2017]'