CBLB (NM_170662) Human Tagged ORF Clone

CAT#: RC206047

CBLB (Myc-DDK-tagged)-Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB)

Product Images

Western blot validation of overexpression lysate (Cat# LY403529) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206047 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CBLB protein (Cat# TP306047). The protein was produced from HEK293T cells transfected with CBLB cDNA clone (Cat# RC206047) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CBLB
• Synonyms: Cbl-b; Nbla00127; RNF56
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206047 representing NM_170662.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCAAACTCAATGAATGGCAGAAACCCTGGTGGTCGAGGAGGAAATCCCCGAAAAGGTCGAATTTTG
  GGTATTATTGATGCTATTCAGGATGCAGTTGGACCCCCTAAGCAAGCTGCCGCAGATCGCAGGACCGTG
  GAGAAGACTTGGAAGCTCATGGACAAAGTGGTAAGACTGTGCCAAAATCCCAAACTTCAGTTGAAAAAT
  AGCCCACCATATATACTTGATATTTTGCCTGATACATATCAGCATTTACGACTTATATTGAGTAAATAT
  GATGACAACCAGAAACTTGCCCAACTCAGTGAGAATGAGTACTTTAAAATCTACATTGATAGCCTTATG
  AAAAAGTCAAAACGGGCAATAAGACTCTTTAAAGAAGGCAAGGAGAGAATGTATGAAGAACAGTCACAG
  GACAGACGAAATCTCACAAAACTGTCCCTTATCTTCAGTCACATGCTGGCAGAAATCAAAGCAATCTTT
  CCCAATGGTCAATTCCAGGGAGATAACTTTCGTATCACAAAAGCAGATGCTGCTGAATTCTGGAGAAAG
  TTTTTTGGAGACAAAACTATCGTACCATGGAAAGTATTCAGACAGTGCCTTCATGAGGTCCACCAGATT
  AGCTCTGGCCTGGAAGCAATGGCTCTAAAATCAACAATTGATTTAACTTGCAATGATTACATTTCAGTT
  TTTGAATTTGATATTTTTACCAGGCTGTTTCAGCCTTGGGGCTCTATTTTGCGGAATTGGAATTTCTTA
  GCTGTGACACATCCAGGTTACATGGCATTTCTCACATATGATGAAGTTAAAGCACGACTACAGAAATAT
  AGCACCAAACCCGGAAGCTATATTTTCCGGTTAAGTTGCACTCGATTGGGACAGTGGGCCATTGGCTAT
  GTGACTGGGGATGGGAATATCTTACAGACCATACCTCATAACAAGCCCTTATTTCAAGCCCTGATTGAT
  GGCAGCAGGGAAGGATTTTATCTTTATCCTGATGGGAGGAGTTATAATCCTGATTTAACTGGATTATGT
  GAACCTACACCTCATGACCATATAAAAGTTACACAGGAACAATATGAATTATATTGTGAAATGGGCTCC
  ACTTTTCAGCTCTGTAAGATTTGTGCAGAGAATGACAAAGATGTCAAGATTGAGCCTTGTGGGCATTTG
  ATGTGCACCTCTTGCCTTACGGCATGGCAGGAGTCGGATGGTCAGGGCTGCCCTTTCTGTCGTTGTGAA
  ATAAAAGGAACTGAGCCCATAATCGTGGATCCCTTTGATCCAAGAGATGAAGGCTCCAGGTGTTGCAGC
  ATCATTGACCCCTTTGGCATGCCGATGCTCGACTTGGACGACGATGATGATCGTGAGGAGTCCTTGATG
  ATGAATCGGTTGGCAAACGTCCGAAAGTGCACTGACAGGCAGAACTCACCAGTCACATCACCAGGATCC
  TCTCCCCTTGCCCAGAGAAGAAAGCCACAGCCTGACCCACTCCAGATCCCACATCTAAGCCTGCCACCC
  GTGCCTCCTCGCCTGGATCTAATTCAGAAAGGCATAGTTAGATCTCCCTGTGGCAGCCCAACGGGTTCA
  CCAAAGTCTTCTCCTTGCATGGTGAGAAAACAAGATAAACCACTCCCAGCACCACCTCCTCCCTTAAGA
  GATCCTCCTCCACCGCCACCTGAAAGACCTCCACCAATCCCACCAGACAATAGACTGAGTAGACACATC
  CATCATGTGGAAAGCGTGCCTTCCAAAGACCCGCCAATGCCTCTTGAAGCATGGTGCCCTCGGGATGTG
  TTTGGGACTAATCAGCTTGTGGGATGTCGACTCCTAGGGGAGGGCTCTCCAAAACCTGGAATCACAGCG
  AGTTCAAATGTCAATGGAAGGCACAGTAGAGTGGGCTCTGACCCAGTGCTTATGCGGAAACACAGACGC
  CATGATTTGCCTTTAGAAGGAGCTAAGGTCTTTTCCAATGGTCACCTTGGAAGTGAAGAATATGATGTT
  CCTCCCCGGCTTTCTCCTCCTCCTCCAGTTACCACCCTCCTCCCTAGCATAAAGTGTACTGGTCCGTTA
  GCAAATTCTCTTTCAGAGAAAACAAGAGACCCAGTAGAGGAAGATGATGATGAATACAAGATTCCTTCA
  TCCCACCCTGTTTCCCTGAATTCACAACCATCTCATTGTCATAATGTAAAACCTCCTGTTCGGTCTTGT
  GATAATGGTCACTGTATGCTGAATGGAACACATGGTCCATCTTCAGAGAAGAAATCAAACATCCCTGAC
  TTAAGCATATATTTAAAGGGAGATGTTTTTGATTCAGCCTCTGATCCCGTGCCATTACCACCTGCCAGG
  CCTCCAACTCGGGACAATCCAAAGCATGGTTCTTCACTCAACAGGACGCCCTCTGATTATGATCTTCTC
  ATCCCTCCATTAGGTGAAGATGCTTTTGATGCCCTCCCTCCATCTCTCCCACCTCCCCCACCTCCTGCA
  AGGCATAGTCTCATTGAACATTCAAAACCTCCTGGCTCCAGTAGCCGGCCATCCTCAGGACAGGATCTT
  TTTCTTCTTCCTTCAGATCCCTTTGTTGATCTAGCAAGTGGCCAAGTTCCTTTGCCTCCCGCTAGAAGG
  TTACCAGGTGAAAATGTCAAAACTAACAGAACATCACAGGACTATGATCAGCTTCCTTCATGTTCAGAT
  GGTTCACAGGCACCAGCCAGACCCCCTAAACCACGACCGCGCAGGACTGCACCAGAAATTCACCACAGA
  AAACCCCATGGGCCTGAGGCGGCATTGGAAAATGTCGATGCAAAAATTGCAAAACTCATGGGAGAGGGT
  TATGCCTTTGAAGAGGTGAAGAGAGCCTTAGAGATAGCCCAGAATAATGTCGAAGTTGCCCGGAGCATC
  CTCCGAGAATTTGCCTTCCCTCCTCCAGTATCCCCACGTCTAAATCTA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC206047
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MANSMNGRNPGGRGGNPRKGRILGIIDAIQDAVGPPKQAAADRRTVEKTWKLMDKVVRLCQNPKLQLKN
  SPPYILDILPDTYQHLRLILSKYDDNQKLAQLSENEYFKIYIDSLMKKSKRAIRLFKEGKERMYEEQSQ
  DRRNLTKLSLIFSHMLAEIKAIFPNGQFQGDNFRITKADAAEFWRKFFGDKTIVPWKVFRQCLHEVHQI
  SSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWGSILRNWNFLAVTHPGYMAFLTYDEVKARLQKY
  STKPGSYIFRLSCTRLGQWAIGYVTGDGNILQTIPHNKPLFQALIDGSREGFYLYPDGRSYNPDLTGLC
  EPTPHDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESDGQGCPFCRCE
  IKGTEPIIVDPFDPRDEGSRCCSIIDPFGMPMLDLDDDDDREESLMMNRLANVRKCTDRQNSPVTSPGS
  SPLAQRRKPQPDPLQIPHLSLPPVPPRLDLIQKGIVRSPCGSPTGSPKSSPCMVRKQDKPLPAPPPPLR
  DPPPPPPERPPPIPPDNRLSRHIHHVESVPSKDPPMPLEAWCPRDVFGTNQLVGCRLLGEGSPKPGITA
  SSNVNGRHSRVGSDPVLMRKHRRHDLPLEGAKVFSNGHLGSEEYDVPPRLSPPPPVTTLLPSIKCTGPL
  ANSLSEKTRDPVEEDDDEYKIPSSHPVSLNSQPSHCHNVKPPVRSCDNGHCMLNGTHGPSSEKKSNIPD
  LSIYLKGDVFDSASDPVPLPPARPPTRDNPKHGSSLNRTPSDYDLLIPPLGEDAFDALPPSLPPPPPPA
  RHSLIEHSKPPGSSSRPSSGQDLFLLPSDPFVDLASGQVPLPPARRLPGENVKTNRTSQDYDQLPSCSD
  GSQAPARPPKPRPRRTAPEIHHRKPHGPEAALENVDAKIAKLMGEGYAFEEVKRALEIAQNNVEVARSI
  LREFAFPPPVSPRLNL
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC206047 also available, TP306047M
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_170662
• ORF Size: 2946 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170662.2
• RefSeq Size: 3976 bp
• RefSeq ORF: 2949 bp
• Locus ID: 868
• UniProt ID: Q13191
• Cytogenetics: 3q13.11
• Protein Families: Druggable Genome
• Protein Pathways: Chronic myeloid leukemia, Endocytosis, ErbB signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, Pathways in cancer, T cell receptor signaling pathway, Ubiquitin mediated proteolysis
• MW: 109.4 kDa
• Gene Summary: This gene encodes an E3 ubiquitin-protein ligase which promotes proteosome-mediated protein degradation by transferring ubiquitin from an E2 ubiquitin-conjugating enzyme to a substrate. The encoded protein is involved in the regulation of immune response by limiting T-cell receptor, B-cell receptor, and high affinity immunoglobulin epsilon receptor activation. Studies in mouse suggest that this gene is involved in antifungal host defense and that its inhibition leads to increased fungal killing. Manipulation of this gene may be beneficial in implementing immunotherapies for a variety of conditions, including cancer, autoimmune diseases, allergies, and infections. [provided by RefSeq, Sep 2017]