CBLB (NM_170662) Human Untagged Clone

CAT#: SC107022

CBLB (untagged)-Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB)


  "NM_170662" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CBLB
Synonyms Cbl-b; Nbla00127; RNF56
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC107022 sequence for NM_170662 edited (data generated by NextGen Sequencing)
ATGGCAAACTCAATGAATGGCAGAAACCCTGGTGGTCGAGGAGGAAATCCCCGAAAAGGT
CGAATTTTGGGTATTATTGATGCTATTCAGGATGCAGTTGGACCCCCTAAGCAAGCTGCC
GCAGATCGCAGGACCGTGGAGAAGACTTGGAAGCTCATGGACAAAGTGGTAAGACTGTGC
CAAAATCCCAAACTTCAGTTGAAAAATAGCCCACCATATATACTTGATATTTTGCCTGAT
ACATATCAGCATTTACGACTTATATTGAGTAAATATGATGACAACCAGAAACTTGCCCAA
CTCAGTGAGAATGAGTACTTTAAAATCTACATTGATAGCCTTATGAAAAAGTCAAAACGG
GCAATAAGACTCTTTAAAGAAGGCAAGGAGAGAATGTATGAAGAACAGTCACAGGACAGA
CGAAATCTCACAAAACTGTCCCTTATCTTCAGTCACATGCTGGCAGAAATCAAAGCAATC
TTTCCCAATGGTCAATTCCAGGGAGATAACTTTCGTATCACAAAAGCAGATGCTGCTGAA
TTCTGGAGAAAGTTTTTTGGAGACAAAACTATCGTACCATGGAAAGTATTCAGACAGTGC
CTTCATGAGGTCCACCAGATTAGCTCTGGCCTGGAAGCAATGGCTCTAAAATCAACAATT
GATTTAACTTGCAATGATTACATTTCAGTTTTTGAATTTGATATTTTTACCAGGCTGTTT
CAGCCTTGGGGCTCTATTTTGCGGAATTGGAATTTCTTAGCTGTGACACATCCAGGTTAC
ATGGCATTTCTCACATATGATGAAGTTAAAGCACGACTACAGAAATATAGCACCAAACCC
GGAAGCTATATTTTCCGGTTAAGTTGCACTCGATTGGGACAGTGGGCCATTGGCTATGTG
ACTGGGGATGGGAATATCTTACAGACCATACCTCATAACAAGCCCTTATTTCAAGCCCTG
ATTGATGGCAGCAGGGAAGGATTTTATCTTTATCCTGATGGGAGGAGTTATAATCCTGAT
TTAACTGGATTATGTGAACCTACACCTCATGACCATATAAAAGTTACACAGGAACAATAT
GAATTATATTGTGAAATGGGCTCCACTTTTCAGCTCTGTAAGATTTGTGCAGAGAATGAC
AAAGATGTCAAGATTGAGCCTTGTGGGCATTTGATGTGCACCTCTTGCCTTACGGCATGG
CAGGAGTCGGATGGTCAGGGCTGCCCTTTCTGTCGTTGTGAAATAAAAGGAACTGAGCCC
ATAATCGTGGACCCCTTTGATCCAAGAGATGAAGGCTCCAGGTGTTGCAGCATCATTGAC
CCCTTTGGCATGCCGATGCTAGACTTGGACGACGATGATGATCGTGAGGAGTCCTTGATG
ATGAATCGGTTGGCAAACGTCCGAAAGTGCACTGACAGGCAGAACTCACCAGTCACATCA
CCAGGATCCTCTCCCCTTGCCCAGAGAAGAAAGCCACAGCCTGACCCACTCCAGATCCCA
CATCTAAGCCTGCCACCCGTGCCTCCTCGCCTGGATCTAATTCAGAAAGGCATAGTTAGA
TCTCCCTGTGGCAGCCCAACAGGTTCACCAAAGTCTTCTCCTTGCATGGTGAGAAAACAA
GATAAACCACTCCCAGCACCACCTCCTCCCTTAAGAGATCCTCCTCCACCGCCACCTGAA
AGACCTCCACCAATCCCACCAGACAATAGACTGAGTAGACACATCCATCATGTGGAAAGC
GTGCCTTCCAGAGACCCGCCAATGCCTCTTGAAGCATGGTGCCCTCGGGATGTGTTTGGG
ACTAATCAGCTTGTGGGATGTCGACTCCTAGGGGAGGGCTCTCCAAAACCTGGAATCACA
GCAAGTTCAAATGTCAATGGAAGGCACAGTAGAGTGGGCTCTGACCCAGTGCTTATGCGG
AAACACAGACGCCATGATTTGCCTTTAGAAGGAGCTAAGGTCTTTTCCAATGGTCACCTT
GGAAGTGAAGAATATGATGTTCCTCCCCGGCTTTCTCCTCCTCCTCCAGTTACCACCCTC
CTCCCTAGCATAAAGTGTACTGGTCCGTTAGCAAATTCTCTTTCAGAGAAAACAAGAGAC
CCAGTAGAGGAAGATGATGATGAATACAAGATTCCTTCATCCCACCCTGTTTCCCTGAAT
TCACAACCATCTCATTGTCATAATGTAAAACCTCCTGTTCGGTCTTGTGATAATGGTCAC
TGTATGCTGAATGGAACACATGGTCCATCTTCAGAGAAGAAATCAAACATCCCTGACTTA
AGCATATATTTAAAGGGAGATGTTTTTGATTCAGCCTCTGATCCCGTGCCATTACCACCT
GCCAGGCCTCCAACTCGGGACAATCCAAAGCATGGTTCTTCACTCAACAGGACGCCCTCT
GATTATGATCTTCTCATCCCTCCATTAGGTGAAGATGCTTTTGATGCCCTCCCTCCATCT
CTCCCACCTCCCCCACCTCCTGCAAGGCATAGTCTCATTGAACATTCAAAACCTCCTGGC
TCCAGTAGCCGGCCATCCTCAGGACAGGATCTTTTTCTTCTTCCTTCAGATCCCTTTGTT
GATCTAGCAAGTGGCCAAGTTCCTTTGCCTCCTGCTAGAAGGTTACCAGGTGAAAATGTC
AAAACTAACAGAACATCACAGGACTATGATCAGCTTCCTTCATGTTCAGATGGTTCACAG
GCACCAGCCAGACCCCCTAAACCACGACCGCGCAGGACTGCACCAGAAATTCACCACAGA
AAACCCCATGGGCCTGAGGCGGCATTGGAAAATGTCGATGCAAAAATTGCAAAACTCATG
GGAGAGGGTTATGCCTTTGAAGAGGTGAAGAGAGCCTTAGAGATAGCCCAGAATAATGTC
GAAGTTGCCCGGAGCATCCTCCGAGAATTTGCCTTCCCTCCTCCAGTATCCCCACGTCTA
AATCTATAG

Clone variation with respect to NM_170662.3
1581 g=>a;1863 g=>a
>OriGene 5' read for NM_170662 unedited
GGGCGGCCCGCGAATTCGCACGAGGCCTGCTAGTGCTGCTGCGGCGTCCCGCGGCCTCCC
CGAGTCGGGCGGGAGGGGAGAGCGGGTGTGGATTTGTCTTGACGGTAATTGTTGCGTTTC
CACGTCTCGGAGGCCTGCGCGCTGGGTTGCTCCTTCTTCGGGAGCGAGCTGTTCTCAGCG
ATCCCACTCCCAGCCGGGGCTCCCCACACACACTGGGCTGCGTGCGTGTGGAGTGGGACC
CGCGCACACGCGTGTCTCTGGACAGCTACGGCGCCGAAAGAACTAAAATTCCAGATGGCA
AACTCAATGAATGGCAGAAACCCTGGTGGTCGAGGAGGAAATCCCCGAAAAGGTCGAATT
TTGGGTATTATTGATGCTATTCAGGATGCAGTTGGACCCCCTAAGCAAGCTGCCGCAGAT
CGCAGGACCGTGGAGAAGACTTGGAAGCTCATGGACAAAGTGGTAAGACTGTGCCAAAAT
CCCAAACTTCAGTTGAAAAATAGCCCACCATATATACTTGATATTTTGCCTGATACATAT
CAGCATTTACGACTTATATTGAGTAAATATGATGACAACCAGAAACTTGCCCAACTCAGT
GAGAATGGAGTACTTTAAAATCTACATTGATAGCCTTATGAAAAAGTCAAAACGGGCAAT
AAGACTCTTNANAGAAGGCAAGGGAGAGAATGTATTGAAGAACAGTCACAGGACAGACGA
AATCTCACAAACTGTCCCTAATCTTCAGTCACATGCTGGCAGAATCAAGCATCCTCCCCA
TGGTCATTCCAGGGAGATACTTTCGATACAAAGCAGAGCTGCTGATCTGGGGAAAGAGTT
TTGGAGACAAACATCGACAATGGAAGA
>OriGene 3' read for NM_170662 unedited
NNTTTTTTAGCTTGNACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTT
GCATTAGTTTGATCAGTTGAAAATCAGAACCTTCAAAGCATCTTGTGAATGAGAGAAATG
GAACTGGACCTGGGCAAGGCATGGGGATGGTAGAGATCCATATGAATAAATGATTTAGCT
GTTGATGTGGACAGCAAGAGAGTTGGTGGGAAATGTTACAGCAGACCTGACCACGCTACA
AAGGGTCTGTGAAGAACACAGTACAGGTATCAAAACACCAAAACAAAACTAAACTAAAAA
CAAGAACAAACTGCAACTTTGCCAAACTGTAAACAAGGTAAAGCATTTCACAGGTTCAAA
GTTCAAGGGAAGTAAACGTCTTTAAATTATTTTTGTCAGTTCAACCCTGATTTAAAAATA
TGGCTAGCAAAAACAAGGAAGCCACAGCTGTCGACATCCTGAGCAAGCATCGGTCTCCTT
TGAGAAGCTGCACTCCCAAGCCTCTTCTCAAGCTGCTACACGAGGAGGAGACACTTCTCT
CTTGAATTCCATCTCAGTTCTCTTTATTTCCACACTCTTGGAATACATCGATTGCTTTCC
ATTTTGGTGTCTACAGTTCTGGCTGCTATAGATTTAGACGTGGGGATACTGGAGGAAGGG
AAGGCAAATTCTCGGAGGATGCTNCGGGGCACTTCGACATTATTCTGGGCTATCTCTAAG
GCTCTCTTCACCTCTTCAAGGCATACCCTCTCCATGGAGTTTGCAATTTTGCATCGAATT
TTCCATGCCGCTCAGCCTGGGGGTTTTCTGTGGTGAATTCTGGTGCAGCTTGGCGGCCGG
TTTAGGGGGGTCTGGTTGGCGCTGTAACATTTGACTGAAGAGTGACTAGCCCGGGAGTTC
GGAAGTTGCATTC
Restriction Sites NotI-NotI     
ACCN NM_170662
Insert Size 3580 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_170662.3, NP_733762.2
RefSeq Size 3976 bp
RefSeq ORF 2949 bp
Locus ID 868
Cytogenetics 3q13.11
Protein Families Druggable Genome
Protein Pathways Chronic myeloid leukemia, Endocytosis, ErbB signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, Pathways in cancer, T cell receptor signaling pathway, Ubiquitin mediated proteolysis
Gene Summary 'This gene encodes an E3 ubiquitin-protein ligase which promotes proteosome-mediated protein degradation by transferring ubiquitin from an E2 ubiquitin-conjugating enzyme to a substrate. The encoded protein is involved in the regulation of immune response by limiting T-cell receptor, B-cell receptor, and high affinity immunoglobulin epsilon receptor activation. Studies in mouse suggest that this gene is involved in antifungal host defense and that its inhibition leads to increased fungal killing. Manipulation of this gene may be beneficial in implementing immunotherapies for a variety of conditions, including cancer, autoimmune diseases, allergies, and infections. [provided by RefSeq, Sep 2017]'
Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation from a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Both variants 2 and 3 encode isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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