EDG2 (LPAR1) (NM_057159) Human Tagged ORF Clone

CAT#: RG206066

  • TrueORF®

LPAR1 (GFP-tagged) - Human lysophosphatidic acid receptor 1 (LPAR1), transcript variant 2


  "NM_057159" in other vectors (6)

Reconstitution Protocol

USD 460.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol LPAR1
Synonyms edg-2; EDG2; Gpcr26; GPR26; LPA1; Mrec1.3; rec.1.3; vzg-1; VZG1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG206066 representing NM_057159
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCCATCTCTACTTCCATCCCTGTAATTTCACAGCCCCAGTTCACAGCCATGAATGAACCACAGT
GCTTCTACAACGAGTCCATTGCCTTCTTTTATAACCGAAGTGGAAAGCATCTTGCCACAGAATGGAACAC
AGTCAGCAAGCTGGTGATGGGACTTGGAATCACTGTTTGTATCTTCATCATGTTGGCCAACCTATTGGTC
ATGGTGGCAATCTATGTCAACCGCCGCTTCCATTTTCCTATTTATTACCTAATGGCTAATCTGGCTGCTG
CAGACTTCTTTGCTGGGTTGGCCTACTTCTATCTCATGTTCAACACAGGACCCAATACTCGGAGACTGAC
TGTTAGCACATGGCTCCTTCGTCAGGGCCTCATTGACACCAGCCTGACGGCATCTGTGGCCAACTTACTG
GCTATTGCAATCGAGAGGCACATTACGGTTTTCCGCATGCAGCTCCACACACGGATGAGCAACCGGCGGG
TAGTGGTGGTCATTGTGGTCATCTGGACTATGGCCATCGTTATGGGTGCTATACCCAGTGTGGGCTGGAA
CTGTATCTGTGATATTGAAAATTGTTCCAACATGGCACCCCTCTACAGTGACTCTTACTTAGTCTTCTGG
GCCATTTTCAACTTGGTGACCTTTGTGGTAATGGTGGTTCTCTATGCTCACATCTTTGGCTATGTTCGCC
AGAGGACTATGAGAATGTCTCGGCATAGTTCTGGACCCCGGCGGAATCGGGATACCATGATGAGTCTTCT
GAAGACTGTGGTCATTGTGCTTGGGGCCTTTATCATCTGCTGGACTCCTGGATTGGTTTTGTTACTTCTA
GACGTGTGCTGTCCACAGTGCGACGTGCTGGCCTATGAGAAATTCTTCCTTCTCCTTGCTGAATTCAACT
CTGCCATGAACCCCATCATTTACTCCTACCGCGACAAAGAAATGAGCGCCACCTTTAGGCAGATCCTCTG
CTGCCAGCGCAGTGAGAACCCCACCGGCCCCACAGAAGGCTCAGACCGCTCGGCTTCCTCCCTCAACCAC
ACCATCTTGGCTGGAGTTCACAGCAATGACCACTCTGTGGTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG206066 representing NM_057159
Red=Cloning site Green=Tags(s)

MAAISTSIPVISQPQFTAMNEPQCFYNESIAFFYNRSGKHLATEWNTVSKLVMGLGITVCIFIMLANLLV
MVAIYVNRRFHFPIYYLMANLAAADFFAGLAYFYLMFNTGPNTRRLTVSTWLLRQGLIDTSLTASVANLL
AIAIERHITVFRMQLHTRMSNRRVVVVIVVIWTMAIVMGAIPSVGWNCICDIENCSNMAPLYSDSYLVFW
AIFNLVTFVVMVVLYAHIFGYVRQRTMRMSRHSSGPRRNRDTMMSLLKTVVIVLGAFIICWTPGLVLLLL
DVCCPQCDVLAYEKFFLLLAEFNSAMNPIIYSYRDKEMSATFRQILCCQRSENPTGPTEGSDRSASSLNH
TILAGVHSNDHSVV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_057159
ORF Size 1092 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_057159.2, NP_476500.1
RefSeq Size 3182 bp
RefSeq ORF 1095 bp
Locus ID 1902
Cytogenetics 9q31.3
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Gap junction, Neuroactive ligand-receptor interaction
Gene Summary 'The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors mediate diverse biologic functions, including proliferation, platelet aggregation, smooth muscle contraction, inhibition of neuroblastoma cell differentiation, chemotaxis, and tumor cell invasion. Two transcript variants encoding the same protein have been identified for this gene [provided by RefSeq, Jul 2008]'

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