GCS1 (MOGS) (NM_006302) Human Tagged ORF Clone

CAT#: RG206689

MOGS (GFP-tagged) - Human mannosyl-oligosaccharide glucosidase (MOGS), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MOGS
• Synonyms: CDG2B; CWH41; DER7; GCS1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206689 representing NM_006302
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCGGGGCGAGCGGCGGCGCCGCGCAGTGCCGGCAGAGGGAGTGCGGACAGCCGAGAGGGCGGCTC
  GGGGAGGCCCCGGGCGACGGGACGGCCGGGGCGGCGGGCCGCGTAGCACGGCTGGAGGAGTGGCTCTGGC
  CGTCGTGGTCCTGTCTTTGGCCCTGGGTATGTCGGGGCGCTGGGTGCTGGCGTGGTACCGTGCGCGGCGG
  GCGGTCACGCTGCACTCCGCGCCTCCTGTGTTGCCTGCCGACTCCTCCAGCCCCGCCGTGGCCCCGGACC
  TCTTCTGGGGAACCTACCGCCCTCACGTCTACTTCGGCATGAAGACCCGCAGCCCGAAGCCCCTCCTCAC
  CGGACTGATGTGGGCGCAGCAGGGCACCACCCCGGGGACTCCTAAGCTCAGGCACACGTGTGAGCAGGGG
  GACGGTGTGGGTCCCTATGGCTGGGAGTTCCACGACGGCCTCTCCTTCGGGCGCCAACACATCCAGGATG
  GGGCCTTAAGGCTCACCACTGAGTTCGTCAAGAGGCCTGGGGGTCAGCACGGAGGGGACTGGAGCTGGAG
  AGTGACTGTAGAGCCTCAGGACTCAGGTACTTCTGCCCTCCCTTTGGTCTCCCTGTTCTTCTATGTGGTG
  ACAGATGGCAAGGAAGTCCTACTACCAGAGGTTGGGGCCAAGGGGCAGTTGAAGTTTATCAGTGGGCACA
  CCAGTGAACTTGGTAACTTCCGCTTTACACTTTTGCCACCAACCAGTCCAGGGGATACAGCCCCCAAGTA
  TGGCAGCTACAATGTCTTCTGGACCTCCAACCCAGGACTGCCCCTGCTGACAGAGATGGTAAAGAGTCGC
  CTAAATAGCTGGTTTCAGCATCGGCCCCCAGGGGCCTCCCCTGAACGCTACCTCGGCTTGCCAGGATCCC
  TGAAGTGGGAGGACAGAGGTCCAAGTGGGCAAGGGCAGGGGCAGTTCTTGATACAGCAGGTGACCCTGAA
  AATTCCCATTTCCATAGAGTTTGTGTTTGAATCAGGCAGTGCCCAGGCAGGAGGAAATCAAGCCCTGCCA
  AGACTGGCAGGCAGTCTACTGACCCAGGCCCTGGAGAGCCATGCTGAAGGCTTTAGAGAGCGCTTTGAGA
  AGACCTTCCAGCTGAAGGAGAAGGGCCTGAGCTCTGGCGAGCAGGTTTTGGGTCAGGCTGCCCTCAGCGG
  CCTCCTTGGTGGAATTGGCTACTTCTACGGACAAGGGCTGGTATTGCCAGACATCGGGGTGGAAGGGTCT
  GAGCAGAAGGTGGACCCAGCCCTCTTTCCACCCGTACCTCTTTTTACAGCAGTGCCCTCCCGGTCATTCT
  TCCCACGAGGCTTCCTTTGGGATGAAGGCTTTCACCAGCTGGTGGTTCAGCGGTGGGATCCCTCCCTCAC
  CCGGGAAGCCCTTGGCCACTGGCTGGGGCTGCTAAATGCTGATGGCTGGATTGGGAGGGAGCAGATACTG
  GGGGATGAGGCCCGAGCCCGGGTGCCTCCAGAATTCCTAGTACAACGAGCAGTCCACGCCAACCCCCCAA
  CCCTACTTTTGCCTGTAGCCCATATGCTAGAGGTTGGTGACCCTGACGACTTGGCTTTCCTCCGAAAGGC
  CTTGCCCCGCCTGCATGCCTGGTTTTCCTGGCTCCATCAGAGCCAGGCAGGCCCACTGCCACTATCTTAC
  CGCTGGCGGGGACGGGACCCTGCCTTACCAACCTTACTGAACCCCAAGACCCTACCCTCTGGGCTGGATG
  ACTACCCCCGGGCTTCACACCCTTCAGTAACCGAGCGGCACCTGGACCTGCGATGTTGGGTGGCACTGGG
  TGCCCGTGTGCTGACGCGGCTGGCAGAGCATCTGGGTGAGGCTGAGGTAGCTGCTGAGCTGGGCCCACTG
  GCTGCCTCACTGGAGGCAGCAGAGAGCCTGGATGAGCTGCACTGGGCCCCAGAGCTAGGAGTCTTTGCAG
  ACTTTGGGAACCACACAAAAGCAGTACAGCTGAAGCCCAGGCCCCCTCAGGGGCTCGTTCGGGTGGTGGG
  TCGGCCCCAACCTCAACTGCAGTATGTAGATGCTCTTGGCTATGTCAGTCTTTTTCCCTTGCTGCTGCGA
  CTGCTGGACCCCACCTCATCCCGCCTTGGGCCCCTGCTGGACATTCTAGCCGACAGCCGCCATCTCTGGA
  GCCCCTTTGGTTTACGCTCCCTTGCAGCCTCCAGCTCCTTTTATGGCCAGCGCAATTCAGAGCATGATCC
  CCCCTACTGGCGGGGTGCTGTGTGGCTCAATGTCAACTACCTGGCTTTGGGAGCACTCCACCACTATGGG
  CATCTGGAGGGTCCTCACCAGGCTCGGGCTGCCAAACTCCACGGTGAGCTCCGTGCCAACGTGGTAGGCA
  ATGTATGGCGCCAGTACCAGGCTACAGGCTTTCTTTGGGAGCAGTACAGTGACCGCGATGGGCGAGGCAT
  GGGCTGCCGCCCTTTCCACGGCTGGACCAGCCTTGTCTTACTGGCCATGGCTGAAGACTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206689 representing NM_006302
  Red=Cloning site Green=Tags(s)
  
  MARGERRRRAVPAEGVRTAERAARGGPGRRDGRGGGPRSTAGGVALAVVVLSLALGMSGRWVLAWYRARR
  AVTLHSAPPVLPADSSSPAVAPDLFWGTYRPHVYFGMKTRSPKPLLTGLMWAQQGTTPGTPKLRHTCEQG
  DGVGPYGWEFHDGLSFGRQHIQDGALRLTTEFVKRPGGQHGGDWSWRVTVEPQDSGTSALPLVSLFFYVV
  TDGKEVLLPEVGAKGQLKFISGHTSELGNFRFTLLPPTSPGDTAPKYGSYNVFWTSNPGLPLLTEMVKSR
  LNSWFQHRPPGASPERYLGLPGSLKWEDRGPSGQGQGQFLIQQVTLKIPISIEFVFESGSAQAGGNQALP
  RLAGSLLTQALESHAEGFRERFEKTFQLKEKGLSSGEQVLGQAALSGLLGGIGYFYGQGLVLPDIGVEGS
  EQKVDPALFPPVPLFTAVPSRSFFPRGFLWDEGFHQLVVQRWDPSLTREALGHWLGLLNADGWIGREQIL
  GDEARARVPPEFLVQRAVHANPPTLLLPVAHMLEVGDPDDLAFLRKALPRLHAWFSWLHQSQAGPLPLSY
  RWRGRDPALPTLLNPKTLPSGLDDYPRASHPSVTERHLDLRCWVALGARVLTRLAEHLGEAEVAAELGPL
  AASLEAAESLDELHWAPELGVFADFGNHTKAVQLKPRPPQGLVRVVGRPQPQLQYVDALGYVSLFPLLLR
  LLDPTSSRLGPLLDILADSRHLWSPFGLRSLAASSSFYGQRNSEHDPPYWRGAVWLNVNYLALGALHHYG
  HLEGPHQARAAKLHGELRANVVGNVWRQYQATGFLWEQYSDRDGRGMGCRPFHGWTSLVLLAMAEDY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006302
• ORF Size: 2511 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006302.2, NP_006293.2
• RefSeq Size: 2910
• RefSeq ORF: 2514
• Locus ID: 7841
• Domains: Glyco_hydro_63
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: This gene encodes the first enzyme in the N-linked oligosaccharide processing pathway. The enzyme cleaves the distal alpha-1,2-linked glucose residue from the Glc(3)-Man(9)-GlcNAc(2) oligosaccharide precursor. This protein is located in the lumen of the endoplasmic reticulum. Defects in this gene are a cause of type IIb congenital disorder of glycosylation (CDGIIb). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]