Kir2.2 (KCNJ12) (NM_021012) Human Tagged ORF Clone
CAT#: RG207051
- TrueORF®
KCNJ12 (GFP-tagged) - Human potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12)
"NM_021012" in other vectors (6)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | KCNJ12 |
Synonyms | hIRK; hIRK1; hkir2.2x; IRK-2; IRK2; kcnj12x; KCNJN1; Kir2.2; Kir2.2v |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG207051 representing NM_021012
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGCGGCCAGCCGGGCCAACCCCTACAGCATCGTGTCATCGGAGGAGGACGGGCTGCACCTGGTCA CCATGTCGGGCGCCAACGGCTTCGGCAACGGCAAGGTGCACACGCGGCGCAGGTGCCGCAACCGCTTCGT CAAGAAGAATGGCCAGTGCAACATTGAGTTCGCCAACATGGACGAGAAGTCACAGCGCTACCTGGCTGAC ATGTTCACCACCTGTGTGGACATCCGCTGGCGGTACATGCTGCTCATCTTCTCGCTGGCCTTCCTTGCCT CCTGGCTGCTGTTCGGCATCATCTTCTGGGTCATCGCGGTGGCACACGGTGACCTGGAGCCGGCTGAGGG CCGGGGCCGCACACCCTGTGTGATGCAGGTGCACGGCTTCATGGCGGCCTTCCTCTTCTCCATCGAGACG CAGACCACCATCGGCTACGGGCTGCGCTGTGTGACGGAGGAGTGCCCGGTGGCCGTCTTCATGGTGGTGG CCCAGTCCATCGTGGGCTGCATCATCGACTCCTTCATGATTGGTGCCATCATGGCCAAGATGGCAAGGCC CAAGAAGCGGGCACAGACGCTGCTGTTCAGCCACAACGCCGTGGTGGCCCTGCGTGACGGCAAGCTCTGC CTCATGTGGCGTGTGGGTAACCTGCGCAAGAGCCACATTGTGGAGGCCCATGTGCGCGCGCAGCTCATCA AGCCGCGGGTCACCGAGGAGGGCGAGTACATCCCGCTGGACCAGATCGACATCGATGTGGGCTTCGACAA GGGCCTGGACCGCATCTTTCTGGTGTCGCCCATCACCATCTTGCATGAGATTGACGAGGCCAGCCCGCTC TTCGGCATCAGCCGGCAGGACCTGGAGACGGACGACTTTGAGATCGTGGTCATCCTGGAAGGCATGGTGG AGGCCACAGCCATGACCACCCAGGCCCGCAGCTCCTACCTGGCCAATGAGATCCTGTGGGGTCACCGCTT TGAGCCCGTGCTCTTCGAGGAGAAGAACCAGTACAAGATTGACTACTCGCACTTCCACAAGACCTATGAG GTGCCCTCTACGCCCCGCTGCAGTGCGAAGGATCTGGTAGAGAACAAGTTCCTGCTGCCCAGCGCCAACT CCTTCTGCTACGAGAACGAGCTGGCCTTCCTGAGCCGTGACGAGGAGGATGAGGCGGACGGAGACCAGGA CGGCCGAAGCCGGGACGGCCTCAGCCCCCAGGCCAGGCATGACTTTGACAGACTCCAGGCTGGCGGCGGG GTCCTGGAGCAGCGGCCCTACAGACGGGAGTCAGAGATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207051 representing NM_021012
Red=Cloning site Green=Tags(s) MTAASRANPYSIVSSEEDGLHLVTMSGANGFGNGKVHTRRRCRNRFVKKNGQCNIEFANMDEKSQRYLAD MFTTCVDIRWRYMLLIFSLAFLASWLLFGIIFWVIAVAHGDLEPAEGRGRTPCVMQVHGFMAAFLFSIET QTTIGYGLRCVTEECPVAVFMVVAQSIVGCIIDSFMIGAIMAKMARPKKRAQTLLFSHNAVVALRDGKLC LMWRVGNLRKSHIVEAHVRAQLIKPRVTEEGEYIPLDQIDIDVGFDKGLDRIFLVSPITILHEIDEASPL FGISRQDLETDDFEIVVILEGMVEATAMTTQARSSYLANEILWGHRFEPVLFEEKNQYKIDYSHFHKTYE VPSTPRCSAKDLVENKFLLPSANSFCYENELAFLSRDEEDEADGDQDGRSRDGLSPQARHDFDRLQAGGG VLEQRPYRRESEI TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_021012 |
ORF Size | 1299 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021012.4, NP_066292.2 |
RefSeq Size | 5230 bp |
RefSeq ORF | 1302 bp |
Locus ID | 3768 |
Cytogenetics | 17p11.2 |
Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
Gene Summary | 'This gene encodes an inwardly rectifying K+ channel which may be blocked by divalent cations. This protein is thought to be one of multiple inwardly rectifying channels which contribute to the cardiac inward rectifier current (IK1). The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC124157 | KCNJ12 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12) |
USD 310.00 |
|
RC207051 | KCNJ12 (Myc-DDK-tagged)-Human potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12) |
USD 420.00 |
|
RC207051L1 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12), Myc-DDK-tagged |
USD 768.00 |
|
RC207051L2 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12), mGFP tagged |
USD 620.00 |
|
RC207051L3 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12), Myc-DDK-tagged |
USD 620.00 |
|
RC207051L4 | Lenti ORF clone of Human potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12), mGFP tagged |
USD 620.00 |
{0} Product Review(s)
Be the first one to submit a review