Kir2.2 (KCNJ12) (NM_021012) Human Untagged Clone

CAT#: SC124157

KCNJ12 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNJ12
• Synonyms: hIRK; hIRK1; hkir2.2x; IRK-2; IRK2; kcnj12x; KCNJN1; Kir2.2; Kir2.2v
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124157 sequence for NM_021012 edited (data generated by NextGen Sequencing)
  ATGACCGCGGCCAGCCGGGCCAACCCCTACAGCATCGTGTCATCGGAGGAGGACGGGCTG
  CACCTGGTCACCATGTCGGGCGCCAACGGCTTCGGCAACGGCAAGGTGCACACGCGGCGC
  AGGTGCCGCAACCGCTTCGTCAAGAAGAATGGCCAGTGCAACATTGAGTTCGCCAACATG
  GACGAGAAGTCACAGCGCTACCTGGCTGACATGTTCACCACCTGTGTGGACATCCGCTGG
  CGGTACATGCTGCTCATCTTCTCGCTGGCCTTCCTTGCCTCCTGGCTGCTGTTCGGCATC
  ATCTTCTGGGTCATCGCGGTGGCACACGGTGACCTGGAGCCGGCTGAGGGCCGGGGCCGC
  ACACCCTGTGTGATGCAGGTGCACGGCTTCATGGCGGCCTTCCTCTTCTCCATCGAGACG
  CAGACCACCATCGGCTACGGGCTGCGCTGTGTGACGGAGGAGTGCCCGGTGGCCGTCTTC
  ATGGTGGTGGCCCAGTCCATCGTGGGCTGCATCATCGACTCCTTCATGATTGGTGCCATC
  ATGGCCAAGATGGCAAGGCCCAAGAAGCGGGCACAGACGCTGCTGTTCAGCCACAACGCC
  GTGGTGGCCCTGCGTGACGGCAAGCTCTGCCTCATGTGGCGTGTGGGTAACCTGCGCAAG
  AGCCACATTGTGGAGGCCCATGTGCGCGCGCAGCTCATCAAGCCGCGGGTCACCGAGGAG
  GGCGAGTACATCCCGCTGGACCAGATCGACATCGATGTGGGCTTCGACAAGGGCCTGGAC
  CGCATCTTTCTGGTGTCGCCCATCACCATCTTGCATGAGATTGACGAGGCCAGCCCGCTC
  TTCGGCATCAGCCGGCAGGACCTGGAGACGGACGACTTTGAGATCGTGGTCATCCTGGAA
  GGCATGGTGGAGGCCACAGCCATGACCACCCAGGCCCGCAGCTCCTACCTGGCCAATGAG
  ATCCTGTGGGGTCACCGCTTTGAGCCCGTGCTCTTCGAGGAGAAGAACCAGTACAAGATT
  GACTACTCGCACTTCCACAAGACCTATGAGGTGCCCTCTACGCCCCGCTGCAGTGCGAAG
  GATCTGGTAGAGAACAAGTTCCTGCTGCCCAGCGCCAACTCCTTCTGCTACGAGAACGAG
  CTGGCCTTCCTGAGCCGTGACGAGGAGGATGAGGCGGACGGAGACCAGGACGGCCGAAGC
  CGGGACGGCCTCAGCCCCCAGGCCAGGCATGACTTTGACAGACTCCAGGCTGGCGGCGGG
  GTCCTGGAGCAGCGGCCCTACAGACGGGAGTCAGAGATCTGA
  
  Clone variation with respect to NM_021012.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021012 unedited
  NNNGGTGCCGTTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGC
  GCGCCGCGCCCGGGCCACTGACCGAGGATGTTCTAGTGACTGGATCCTTTCCAGTTGGTG
  CCTGGGAAATGGCAGCCACTACTAATTCAGCCTTGAAGACAGTACCGTGCCCACTCAGCA
  CCATTACATAGAAGATAGAACTCAAGAGGAGCCGCCCTGCCTGGAGCTAGCCTGGGGGCG
  AGCCAGGGTCCCCCAACCCCCGGGATGACCGCGGCCAGCCGGGCCAACCCCTACAGCATC
  GTGTCATCGGAGGAGGACGGGCTGCACCTGGTCACCATGTCGGGCGCCAACGGCTTCGGC
  AACGGCAAGGTGCACACGCGGCGCAGGTGCCGCAACCGCTTCGTCAAGAAGAATGGCCAG
  TGCAACATTGAGTTCGCCAACATGGACGAGAAGTCACAGCGCTACCTGGCTGACATGTTC
  ACCACCTGTGTGGACATCCGCTGGCGGTACATGCTGCTCATCTTCTCGCTGGCCTTCCTT
  GCCTCCTGGCTGCTGTTCGGCATCATCTTCTGGGTCATCGCGGTGGCACACGGTGACCTG
  GAGCCGGCTGAGGGCCGGGGCCGCACACCCTGTGTGATGCAGGTGCACGGCTTCATGGCG
  GCCTTCCTCTTCTCCATCGAGACGCAGACCACCATCGGCTACGGGCTGCGCTGTGTGACG
  GAGGAGTGCCCGGTGGCCGTCTTCATGGTGGTGGCCCAGTCCATCGTGGGCTGCATCATC
  GACTCCTTCATGATTGGTGCCATCATGGCCAAGATGGCAAGGCCCAAGAAGCGGGCACAG
  ACGCTGCTGTTCAGCCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021012 unedited
  TCAACTTCACGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTG
  TTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTT
  GTAAACAGTCTCACCCAAGCTAAGGTCAAGAAAGCTGAAACCCGGTGTTTCTCCCCAAGT
  TAAAAACCAGCAAGGAGCACCGCAGCTAGCCAGCCTTGGACAGCAGAGGCTGGGCCCACC
  ATCGAGCTGAGCTGCTTCCCCGCAGGCTGCAGCTCCATCTTCAGGCAGAAGCCCTGGCCC
  TCGTGGCCTGGCCCCCCAGGAGGCCACTTCTTTGCCCTGAGCCTGTGATAGCCTCTGAGG
  CCGGGGGCTGCCGCTCTGGCTGCCCCCTCTCCGGCCAAACTTCTGAGGCCGTTGCAAAGA
  AACATAAAACGACTAANACGCTACTGAGTCTGCAACCCAGGGCACTGGGCCCGTTCTGCT
  CAAACCCGGGGCCCCTGAGCGACCGCGGNGCCTCTCCCCAGCCGGGGGTGGATGCTGCAT
  GTCGGCCAAGGTTGGCTCAGATCTCTGACTCCCGTCTGTAGGGCCGCTGCTCCAGGACCC
  CGCCGCCAGCCTGGAGTCTGTCAAAGTCATGCCTGGCCTGGGGGCTGANGCCGTCCCGGC
  TTCNGCCGTCCTGGTCTCCGTCCGCCTCATCCTCCTCGTCACGGCTAAGGAANGGCCCAC
  TCGTTCTCGTAACAAAAGGAGTTGGCGCTGGGC
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_021012
• Insert Size: 2100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021012.3, NP_066292.2
• RefSeq Size: 1796 bp
• RefSeq ORF: 1302 bp
• Locus ID: 3768
• Cytogenetics: 17p11.2
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'This gene encodes an inwardly rectifying K+ channel which may be blocked by divalent cations. This protein is thought to be one of multiple inwardly rectifying channels which contribute to the cardiac inward rectifier current (IK1). The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]'