Kir2.2 (KCNJ12) (NM_021012) Human Untagged Clone

CAT#: SC124157

KCNJ12 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 12 (KCNJ12)


  "NM_021012" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol KCNJ12
Synonyms hIRK; hIRK1; hkir2.2x; IRK-2; IRK2; kcnj12x; KCNJN1; Kir2.2; Kir2.2v
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC124157 sequence for NM_021012 edited (data generated by NextGen Sequencing)
ATGACCGCGGCCAGCCGGGCCAACCCCTACAGCATCGTGTCATCGGAGGAGGACGGGCTG
CACCTGGTCACCATGTCGGGCGCCAACGGCTTCGGCAACGGCAAGGTGCACACGCGGCGC
AGGTGCCGCAACCGCTTCGTCAAGAAGAATGGCCAGTGCAACATTGAGTTCGCCAACATG
GACGAGAAGTCACAGCGCTACCTGGCTGACATGTTCACCACCTGTGTGGACATCCGCTGG
CGGTACATGCTGCTCATCTTCTCGCTGGCCTTCCTTGCCTCCTGGCTGCTGTTCGGCATC
ATCTTCTGGGTCATCGCGGTGGCACACGGTGACCTGGAGCCGGCTGAGGGCCGGGGCCGC
ACACCCTGTGTGATGCAGGTGCACGGCTTCATGGCGGCCTTCCTCTTCTCCATCGAGACG
CAGACCACCATCGGCTACGGGCTGCGCTGTGTGACGGAGGAGTGCCCGGTGGCCGTCTTC
ATGGTGGTGGCCCAGTCCATCGTGGGCTGCATCATCGACTCCTTCATGATTGGTGCCATC
ATGGCCAAGATGGCAAGGCCCAAGAAGCGGGCACAGACGCTGCTGTTCAGCCACAACGCC
GTGGTGGCCCTGCGTGACGGCAAGCTCTGCCTCATGTGGCGTGTGGGTAACCTGCGCAAG
AGCCACATTGTGGAGGCCCATGTGCGCGCGCAGCTCATCAAGCCGCGGGTCACCGAGGAG
GGCGAGTACATCCCGCTGGACCAGATCGACATCGATGTGGGCTTCGACAAGGGCCTGGAC
CGCATCTTTCTGGTGTCGCCCATCACCATCTTGCATGAGATTGACGAGGCCAGCCCGCTC
TTCGGCATCAGCCGGCAGGACCTGGAGACGGACGACTTTGAGATCGTGGTCATCCTGGAA
GGCATGGTGGAGGCCACAGCCATGACCACCCAGGCCCGCAGCTCCTACCTGGCCAATGAG
ATCCTGTGGGGTCACCGCTTTGAGCCCGTGCTCTTCGAGGAGAAGAACCAGTACAAGATT
GACTACTCGCACTTCCACAAGACCTATGAGGTGCCCTCTACGCCCCGCTGCAGTGCGAAG
GATCTGGTAGAGAACAAGTTCCTGCTGCCCAGCGCCAACTCCTTCTGCTACGAGAACGAG
CTGGCCTTCCTGAGCCGTGACGAGGAGGATGAGGCGGACGGAGACCAGGACGGCCGAAGC
CGGGACGGCCTCAGCCCCCAGGCCAGGCATGACTTTGACAGACTCCAGGCTGGCGGCGGG
GTCCTGGAGCAGCGGCCCTACAGACGGGAGTCAGAGATCTGA

Clone variation with respect to NM_021012.4
>OriGene 5' read for NM_021012 unedited
NNNGGTGCCGTTCAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGC
GCGCCGCGCCCGGGCCACTGACCGAGGATGTTCTAGTGACTGGATCCTTTCCAGTTGGTG
CCTGGGAAATGGCAGCCACTACTAATTCAGCCTTGAAGACAGTACCGTGCCCACTCAGCA
CCATTACATAGAAGATAGAACTCAAGAGGAGCCGCCCTGCCTGGAGCTAGCCTGGGGGCG
AGCCAGGGTCCCCCAACCCCCGGGATGACCGCGGCCAGCCGGGCCAACCCCTACAGCATC
GTGTCATCGGAGGAGGACGGGCTGCACCTGGTCACCATGTCGGGCGCCAACGGCTTCGGC
AACGGCAAGGTGCACACGCGGCGCAGGTGCCGCAACCGCTTCGTCAAGAAGAATGGCCAG
TGCAACATTGAGTTCGCCAACATGGACGAGAAGTCACAGCGCTACCTGGCTGACATGTTC
ACCACCTGTGTGGACATCCGCTGGCGGTACATGCTGCTCATCTTCTCGCTGGCCTTCCTT
GCCTCCTGGCTGCTGTTCGGCATCATCTTCTGGGTCATCGCGGTGGCACACGGTGACCTG
GAGCCGGCTGAGGGCCGGGGCCGCACACCCTGTGTGATGCAGGTGCACGGCTTCATGGCG
GCCTTCCTCTTCTCCATCGAGACGCAGACCACCATCGGCTACGGGCTGCGCTGTGTGACG
GAGGAGTGCCCGGTGGCCGTCTTCATGGTGGTGGCCCAGTCCATCGTGGGCTGCATCATC
GACTCCTTCATGATTGGTGCCATCATGGCCAAGATGGCAAGGCCCAAGAAGCGGGCACAG
ACGCTGCTGTTCAGCCAA
>OriGene 3' read for NM_021012 unedited
TCAACTTCACGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTG
TTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTT
GTAAACAGTCTCACCCAAGCTAAGGTCAAGAAAGCTGAAACCCGGTGTTTCTCCCCAAGT
TAAAAACCAGCAAGGAGCACCGCAGCTAGCCAGCCTTGGACAGCAGAGGCTGGGCCCACC
ATCGAGCTGAGCTGCTTCCCCGCAGGCTGCAGCTCCATCTTCAGGCAGAAGCCCTGGCCC
TCGTGGCCTGGCCCCCCAGGAGGCCACTTCTTTGCCCTGAGCCTGTGATAGCCTCTGAGG
CCGGGGGCTGCCGCTCTGGCTGCCCCCTCTCCGGCCAAACTTCTGAGGCCGTTGCAAAGA
AACATAAAACGACTAANACGCTACTGAGTCTGCAACCCAGGGCACTGGGCCCGTTCTGCT
CAAACCCGGGGCCCCTGAGCGACCGCGGNGCCTCTCCCCAGCCGGGGGTGGATGCTGCAT
GTCGGCCAAGGTTGGCTCAGATCTCTGACTCCCGTCTGTAGGGCCGCTGCTCCAGGACCC
CGCCGCCAGCCTGGAGTCTGTCAAAGTCATGCCTGGCCTGGGGGCTGANGCCGTCCCGGC
TTCNGCCGTCCTGGTCTCCGTCCGCCTCATCCTCCTCGTCACGGCTAAGGAANGGCCCAC
TCGTTCTCGTAACAAAAGGAGTTGGCGCTGGGC
Restriction Sites ECoRI-NOT     
ACCN NM_021012
Insert Size 2100 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021012.3, NP_066292.2
RefSeq Size 1796 bp
RefSeq ORF 1302 bp
Locus ID 3768
Cytogenetics 17p11.2
Protein Families Druggable Genome, Ion Channels: Potassium, Transmembrane
Gene Summary 'This gene encodes an inwardly rectifying K+ channel which may be blocked by divalent cations. This protein is thought to be one of multiple inwardly rectifying channels which contribute to the cardiac inward rectifier current (IK1). The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]'

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