LAR (PTPRF) (NM_130440) Human Tagged ORF Clone

CAT#: RG207873

PTPRF (GFP-tagged) - Human protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTPRF
• Synonyms: BNAH2; LAR
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207873 representing NM_130440
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCTGAGCCAGCCCCAGGGAGGACGATGGTGCCCCTTGTGCCTGCACTGGTGATGCTTGGTTTGG
  TGGCAGGCGCCCATGGTGACAGCAAACCTGTCTTCATTAAAGTCCCTGAGGACCAGACTGGGCTGTCAGG
  AGGGGTAGCCTCCTTCGTGTGCCAAGCTACAGGAGAACCCAAGCCGCGCATCACATGGATGAAGAAGGGG
  AAGAAAGTCAGCTCCCAGCGCTTCGAGGTCATTGAGTTTGATGATGGGGCAGGGTCAGTGCTTCGGATCC
  AGCCATTGCGGGTGCAGCGAGATGAAGCCATCTATGAGTGTACAGCTACTAACAGCCTGGGTGAGATCAA
  CACTAGTGCCAAGCTCTCAGTGCTCGAAGAGGAACAGCTGCCCCCTGGGTTCCCTTCCATCGACATGGGG
  CCTCAGCTGAAGGTGGTGGAGAAGGCACGCACAGCCACCATGCTATGTGCCGCAGGCGGAAATCCAGACC
  CTGAGATTTCTTGGTTCAAGGACTTCCTTCCTGTAGACCCTGCCACGAGCAACGGCCGCATCAAGCAGCT
  GCGTTCAGGTGCCTTGCAGATAGAGAGCAGTGAGGAATCCGACCAAGGCAAGTACGAGTGTGTGGCGACC
  AACTCGGCAGGCACACGTTACTCAGCCCCTGCGAACCTGTATGTGCGAGTGCGCCGCGTGGCTCCTCGTT
  TCTCCATCCCTCCCAGCAGCCAGGAGGTGATGCCAGGCGGCAGCGTGAACCTGACATGCGTGGCAGTGGG
  TGCACCCATGCCCTACGTGAAGTGGATGATGGGGGCCGAGGAGCTCACCAAGGAGGATGAGATGCCAGTT
  GGCCGCAACGTCCTGGAGCTCAGCAATGTCGTACGCTCTGCCAACTACACCTGTGTGGCCATCTCCTCGC
  TGGGCATGATCGAGGCCACAGCCCAGGTCACAGTGAAAGCTCTTCCAAAGCCTCCGATTGATCTTGTGGT
  GACAGAGACAACTGCCACCAGTGTCACCCTCACCTGGGACTCTGGGAACTCGGAGCCTGTAACCTACTAT
  GGCATCCAGTACCGCGCAGCGGGCACGGAGGGCCCCTTTCAGGAGGTGGATGGTGTGGCCACCACCCGCT
  ACAGCATTGGCGGCCTCAGCCCTTTCTCGGAATATGCCTTCCGCGTGCTGGCGGTGAACAGCATCGGGCG
  AGGGCCGCCCAGCGAGGCAGTGCGGGCACGCACGGGAGAACAGGCGCCCTCCAGCCCACCGCGCCGCGTG
  CAGGCACGCATGCTGAGCGCCAGCACCATGCTGGTGCAGTGGGAGCCTCCCGAGGAGCCCAACGGCCTGG
  TGCGGGGATACCGCGTCTACTATACTCCGGACTCCCGCCGCCCCCCGAACGCCTGGCACAAGCACAACAC
  CGACGCGGGGCTCCTCACGACCGTGGGCAGCCTGCTGCCTGGCATCACCTACAGCCTGCGCGTGCTTGCC
  TTCACCGCCGTGGGCGATGGCCCTCCCAGCCCCACCATCCAGGTCAAGACGCAGCAGGGAGTGCCTGCCC
  AGCCCGCGGACTTCCAGGCCGAGGTGGAGTCGGACACCAGGATCCAGCTCTCGTGGCTGCTGCCCCCTCA
  GGAGCGGATCATCATGTATGAACTGGTGTACTGGGCGGCAGAGGACGAAGACCAACAGCACAAGGTGACC
  TTCGACCCAACCTCCTCCTACACACTAGAGGACCTGAAGCCTGACACACTCTACCGCTTCCAGCTGGCTG
  CACGCTCGGATATGGGGGTGGGCGTCTTCACCCCCACCATTGAGGCCCGCACAGCCCAGTCCACCCCCTC
  CGCCCCTCCCCAGAAGGTGATGTGTGTGAGCATGGGCTCCACCACGGTCCGGGTAAGTTGGGTCCCGCCG
  CCTGCCGACAGCCGCAACGGCGTTATCACCCAGTACTCCGTGGCCTACGAGGCGGTGGACGGCGAGGACC
  GCGGGCGGCATGTGGTGGATGGCATCAGCCGTGAGCACTCCAGCTGGGACCTGGTGGGCCTGGAGAAGTG
  GACGGAGTACCGGGTGTGGGTGCGGGCACACACAGACGTGGGCCCCGGCCCCGAGAGCAGCCCGGTGCTG
  GTGCGCACCGATGAGGACGTGCCCAGCGGGCCTCCGCGGAAGGTGGAGGTGGAGCCACTGAACTCCACTG
  CTGTGCATGTCTACTGGAAGCTGCCTGTCCCCAGCAAGCAGCATGGCCAGATCCGCGGCTACCAGGTCAC
  CTACGTGCGGCTGGAGAATGGCGAGCCCCGTGGACTCCCCATCATCCAAGACGTCATGCTAGCCGAGGCC
  CAGGAAACCACTATCAGCGGCCTGACCCCGGAGACCACCTACTCCGTTACTGTTGCTGCCTATACCACCA
  AGGGGGATGGTGCCCGCAGCAAGCCCAAAATTGTCACTACAACAGGTGCAGTCCCAGGCCGGCCCACCAT
  GATGATCAGCACCACGGCCATGAACACTGCGCTGCTCCAGTGGCACCCACCCAAGGAACTGCCTGGCGAG
  CTGCTGGGCTACCGGCTGCAGTACTGCCGGGCCGACGAGGCGCGGCCCAACACCATAGATTTCGGCAAGG
  ATGACCAGCACTTCACAGTCACCGGCCTGCACAAGGGGACCACCTACATCTTCCGGCTTGCTGCCAAGAA
  CCGGGCTGGCTTGGGTGAGGAGTTCGAGAAGGAGATCAGGACCCCCGAGGACCTGCCCAGCGGCTTCCCC
  CAAAACCTGCATGTGACAGGACTGACCACGTCTACCACAGAACTGGCCTGGGACCCGCCAGTGCTGGCGG
  AGAGGAACGGGCGCATCATCAGCTACACCGTGGTGTTCCGAGACATCAACAGCCAACAGGAGCTGCAGAA
  CATCACGACAGACACCCGCTTTACCCTTACTGGCCTCAAGCCAGACACCACTTACGACATCAAGGTCCGC
  GCATGGACCAGCAAAGGCTCTGGCCCACTCAGCCCCAGCATCCAGTCCCGGACCATGCCGGTGGAGCAAG
  TGTTTGCCAAGAACTTCCGGGTGGCGGCTGCAATGAAGACGTCTGTGCTGCTCAGCTGGGAGGTTCCCGA
  CTCCTATAAGTCAGCTGTGCCCTTTAAGATTCTGTACAATGGGCAGAGTGTGGAGGTGGACGGGCACTCG
  ATGCGGAAGCTGATCGCAGACCTGCAGCCCAACACAGAGTACTCGTTTGTGCTGATGAACCGTGGCAGCA
  GCGCAGGGGGCCTGCAGCACCTGGTGTCCATCCGCACAGCCCCCGACCTCCTGCCTCACAAGCCGCTGCC
  TGCCTCTGCCTACATAGAGGACGGCCGCTTCGATCTCTCCATGCCCCATGTGCAAGACCCCTCGCTTGTC
  AGGTGGTTCTACATTGTTGTGGTGCCCATTGACCGTGTGGGCGGGAGCATGCTGACGCCAAGGTGGAGCA
  CACCCGAGGAACTGGAGCTGGACGAGCTTCTAGAAGCCATCGAGCAAGGCGGAGAGGAGCAGCGGCGGCG
  GCGGCGGCAGGCAGAACGTCTGAAGCCATATGTGGCTGCTCAACTGGATGTGCTCCCGGAGACCTTTACC
  TTGGGGGACAAGAAGAACTACCGGGGCTTCTACAACCGGCCCCTGTCTCCGGACTTGAGCTACCAGTGCT
  TTGTGCTTGCCTCCTTGAAGGAACCCATGGACCAGAAGCGCTATGCCTCCAGCCCCTACTCGGATGAGAT
  CGTGGTCCAGGTGACACCAGCCCAGCAGCAGGAGGAGCCGGAGATGCTGTGGGTGACGGGTCCCGTGCTG
  GCAGTCATCCTCATCATCCTCATTGTCATCGCCATCCTCTTGTTCAAAAGGAAAAGGACCCACTCTCCGT
  CCTCTAAGGATGAGCAGTCGATCGGACTGAAGGACTCCTTGCTGGCCCACTCCTCTGACCCTGTGGAGAT
  GCGGAGGCTCAACTACCAGACCCCAGGTATGCGAGACCACCCACCCATCCCCATCACCGACCTGGCGGAC
  AACATCGAGCGCCTCAAAGCCAACGATGGCCTCAAGTTCTCCCAGGAGTATGAGTCCATCGACCCTGGAC
  AGCAGTTCACGTGGGAGAATTCAAACCTGGAGGTGAACAAGCCCAAGAACCGCTATGCGAATGTCATCGC
  CTACGACCACTCTCGAGTCATCCTTACCTCTATCGATGGCGTCCCCGGGAGTGACTACATCAATGCCAAC
  TACATCGATGGCTACCGCAAGCAGAATGCCTACACCGCCACGCAGGGCCCCCTGCCCGAGACCATGGGTG
  ATTTCTGGAGGATGGTGTGGGAACAGCGCACGGCCACTGTGGTCATGATGACACGGCTGGAGGAGAAGTC
  CCGGGTAAAATGTGATCAGTACTGGCCAGCCCGTGGCACCGAGACCTGTGGCCTTATTCAGGTGACCCTG
  TTGGACACAGTGGAGCTGGCCACATACACTGTGCGCACCTTCGCACTCCACAAGAGTGGCTCCAGTGAGA
  AGCGCGAGCTGCGTCAGTTTCAGTTCATGGCCTGGCCAGACCATGGAGTTCCTGAGTACCCAACTCCCAT
  CCTGGCCTTCCTACGACGGGTCAAGGCCTGCAACCCCCTAGACGCAGGGCCCATGGTGGTGCACTGCAGC
  GCGGGCGTGGGCCGCACCGGCTGCTTCATCGTGATTGATGCCATGTTGGAGCGGATGAAGCACGAGAAGA
  CGGTGGACATCTATGGCCACGTGACCTGCATGCGATCACAGAGGAACTACATGGTGCAGACGGAGGACCA
  GTACGTGTTCATCCATGAGGCGCTGCTGGAGGCTGCCACGTGCGGCCACACAGAGGTGCCTGCCCGCAAC
  CTGTATGCCCACATCCAGAAGCTGGGCCAAGTGCCTCCAGGGGAGAGTGTGACCGCCATGGAGCTCGAGT
  TCAAGTTGCTGGCCAGCTCCAAGGCCCACACGTCCCGCTTCATCAGCGCCAACCTGCCCTGCAACAAGTT
  CAAGAACCGGCTGGTGAACATCATGCCCTACGAATTGACCCGTGTGTGTCTGCAGCCCATCCGTGGTGTG
  GAGGGCTCTGACTACATCAATGCCAGCTTCCTGGATGGTTATAGACAGCAGAAGGCCTACATAGCTACAC
  AGGGGCCTCTGGCAGAGAGCACCGAGGACTTCTGGCGCATGCTATGGGAGCACAATTCCACCATCATCGT
  CATGCTGACCAAGCTTCGGGAGATGGGCAGGGAGAAATGCCACCAGTACTGGCCAGCAGAGCGCTCTGCT
  CGCTACCAGTACTTTGTTGTTGACCCGATGGCTGAGTACAACATGCCCCAGTATATCCTGCGTGAGTTCA
  AGGTCACGGATGCCCGGGATGGGCAGTCAAGGACAATCCGGCAGTTCCAGTTCACAGACTGGCCAGAGCA
  GGGCGTGCCCAAGACAGGCGAGGGATTCATTGACTTCATCGGGCAGGTGCATAAGACCAAGGAGCAGTTT
  GGACAGGATGGGCCTATCACGGTGCACTGCAGTGCTGGCGTGGGCCGCACCGGGGTGTTCATCACTCTGA
  GCATCGTCCTGGAGCGCATGCGCTACGAGGGCGTGGTCGACATGTTTCAGACCGTGAAGACCCTGCGTAC
  ACAGCGTCCTGCCATGGTGCAGACAGAGGACCAGTATCAGCTGTGCTACCGTGCGGCCCTGGAGTACCTC
  GGCAGCTTTGACCACTATGCAACG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207873 representing NM_130440
  Red=Cloning site Green=Tags(s)
  
  MAPEPAPGRTMVPLVPALVMLGLVAGAHGDSKPVFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKG
  KKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMG
  PQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGALQIESSEESDQGKYECVAT
  NSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPV
  GRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLVVTETTATSVTLTWDSGNSEPVTYY
  GIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRV
  QARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDSRRPPNAWHKHNTDAGLLTTVGSLLPGITYSLRVLA
  FTAVGDGPPSPTIQVKTQQGVPAQPADFQAEVESDTRIQLSWLLPPQERIIMYELVYWAAEDEDQQHKVT
  FDPTSSYTLEDLKPDTLYRFQLAARSDMGVGVFTPTIEARTAQSTPSAPPQKVMCVSMGSTTVRVSWVPP
  PADSRNGVITQYSVAYEAVDGEDRGRHVVDGISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVL
  VRTDEDVPSGPPRKVEVEPLNSTAVHVYWKLPVPSKQHGQIRGYQVTYVRLENGEPRGLPIIQDVMLAEA
  QETTISGLTPETTYSVTVAAYTTKGDGARSKPKIVTTTGAVPGRPTMMISTTAMNTALLQWHPPKELPGE
  LLGYRLQYCRADEARPNTIDFGKDDQHFTVTGLHKGTTYIFRLAAKNRAGLGEEFEKEIRTPEDLPSGFP
  QNLHVTGLTTSTTELAWDPPVLAERNGRIISYTVVFRDINSQQELQNITTDTRFTLTGLKPDTTYDIKVR
  AWTSKGSGPLSPSIQSRTMPVEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHS
  MRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPLPASAYIEDGRFDLSMPHVQDPSLV
  RWFYIVVVPIDRVGGSMLTPRWSTPEELELDELLEAIEQGGEEQRRRRRQAERLKPYVAAQLDVLPETFT
  LGDKKNYRGFYNRPLSPDLSYQCFVLASLKEPMDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVL
  AVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLAD
  NIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSIDGVPGSDYINAN
  YIDGYRKQNAYTATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETCGLIQVTL
  LDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCS
  AGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARN
  LYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGV
  EGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHNSTIIVMLTKLREMGREKCHQYWPAERSA
  RYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQF
  GQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYL
  GSFDHYAT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130440
• ORF Size: 5694 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130440.2, NP_569707.2
• RefSeq Size: 7706 bp
• RefSeq ORF: 5697 bp
• Locus ID: 5792
• Cytogenetics: 1p34.2
• Domains: Y_phosphatase, ig, PTPc_motif, IGc2, IG, FN3
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: Adherens junction, Cell adhesion molecules (CAMs), Insulin signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains, and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherents junctions, as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]'