LAR (PTPRF) (NM_130440) Human Untagged Clone

CAT#: SC318687

PTPRF (untagged)-Human protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTPRF
• Synonyms: BNAH2; LAR
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_130440 edited
  GCGGTGGCGGCGGCAGAGGCGGCGGCTCCAGCTTCGGCTCCGGCTCGGGCTCGGGCTCCG
  GCTCCGGCTCCGGCTCCGGCTCCAGCTCGGGTGGCGGTGGCGGGAGCGGGACCAGGTGGA
  GGCGGCGGCGGCAGAGGAGTGGGAGCAGCGGCCCTAGCGGCTTGCGGGGGGACATGCGGA
  CCGACGGCCCCTGGATAGGCGGAAGGAGTGGAGGCCCTGGTGCCCGGCCCTTGGTGCTGA
  GTATCCAGCAAGAGTGACCGGGGTGAAGAAGCAAAGACTCGGTTGATTGTCCTGGGCTGT
  GGCTGGCTGTGGAGCTAGAGCCCTGGATGGCCCCTGAGCCAGCCCCAGGGAGGACGATGG
  TGCCCCTTGTGCCTGCACTGGTGATGCTTGGTTTGGTGGCAGGCGCCCATGGTGACAGCA
  AACCTGTCTTCATTAAAGTCCCTGAGGACCAGACTGGGCTGTCAGGAGGGGTAGCCTCCT
  TCGTGTGCCAAGCTACAGGAGAACCCAAGCCGCGCATCACATGGATGAAGAAGGGGAAGA
  AAGTCAGCTCCCAGCGCTTCGAGGTCATTGAGTTTGATGATGGGGCAGGGTCAGTGCTTC
  GGATCCAGCCATTGCGGGTGCAGCGAGATGAAGCCATCTATGAGTGTACAGCTACTAACA
  GCCTGGGTGAGATCAACACTAGTGCCAAGCTCTCAGTGCTCGAAGAGGAACAGCTGCCCC
  CTGGGTTCCCTTCCATCGACATGGGGCCTCAGCTGAAGGTGGTGGAGAAGGCACGCACAG
  CCACCATGCTATGTGCCGCAGGCGGAAATCCAGACCCTGAGATTTCTTGGTTCAAGGACT
  TCCTTCCTGTAGACCCTGCCACGAGCAACGGCCGCATCAAGCAGCTGCGTTCAGGTGCCT
  TGCAGATAGAGAGCAGTGAGGAATCCGACCAAGGCAAGTACGAGTGTGTGGCGACCAACT
  CGGCAGGCACACGTTACTCAGCCCCTGCGAACCTGTATGTGCGAGTGCGCCGCGTGGCTC
  CTCGTTTCTCCATCCCTCCCAGCAGCCAGGAGGTGATGCCAGGCGGCAGCGTGAACCTGA
  CATGCGTGGCAGTGGGTGCACCCATGCCCTACGTGAAGTGGATGATGGGGGCCGAGGAGC
  TCACCAAGGAGGATGAGATGCCAGTTGGCCGCAACGTCCTGGAGCTCAGCAATGTCGTAC
  GCTCTGCCAACTACACCTGTGTGGCCATCTCCTCGCTGGGCATGATCGAGGCCACAGCCC
  AGGTCACAGTGAAAGCTCTTCCAAAGCCTCCGATTGATCTTGTGGTGACAGAGACAACTG
  CCACCAGTGTCACCCTCACCTGGGACTCTGGGAACTCGGAGCCTGTAACCTACTATGGCA
  TCCAGTACCGCGCAGCGGGCACGGAGGGCCCCTTTCAGGAGGTGGATGGTGTGGCCACCA
  CCCGCTACAGCATTGGCGGCCTCAGCCCTTTCTCGGAATATGCCTTCCGCGTGCTGGCGG
  TGAACAGCATCGGGCGAGGGCCGCCCAGCGAGGCAGTGCGGGCACGCACGGGAGAACAGG
  CGCCCTCCAGCCCACCGCGCCGCGTGCAGGCACGCATGCTGAGCGCCAGCACCATGCTGG
  TGCAGTGGGAGCCTCCCGAGGAGCCCAACGGCCTGGTGCGGGGATACCGCGTCTACTATA
  CTCCGGACTCCCGCCGCCCCCCGAACGCCTGGCACAAGCACAACACCGACGCGGGGCTCC
  TCACGACCGTGGGCAGCCTGCTGCCTGGCATCACCTACAGCCTGCGCGTGCTTGCCTTCA
  CCGCCGTGGGCGATGGCCCTCCCAGCCCCACCATCCAGGTCAAGACGCAGCAGGGAGTGC
  CTGCCCAGCCCGCGGACTTCCAGGCCGAGGTGGAGTCGGACACCAGGATCCAGCTCTCGT
  GGCTGCTGCCCCCTCAGGAGCGGATCATCATGTATGAACTGGTGTACTGGGCGGCAGAGG
  ACGAAGACCAACAGCACAAGGTGACCTTCGACCCAACCTCCTCCTACACACTAGAGGACC
  TGAAGCCTGACACACTCTACCGCTTCCAGCTGGCTGCACGCTCGGATATGGGGGTGGGCG
  TCTTCACCCCCACCATTGAGGCCCGCACAGCCCAGTCCACCCCCTCCGCCCCTCCCCAGA
  AGGTGATGTGTGTGAGCATGGGCTCCACCACGGTCCGGGTAAGTTGGGTCCCGCCGCCTG
  CCGACAGCCGCAACGGCGTTATCACCCAGTACTCCGTGGCCTACGAGGCGGTGGACGGCG
  AGGACCGCGGGCGGCATGTGGTGGATGGCATCAGCCGTGAGCACTCCAGCTGGGACCTGG
  TGGGCCTGGAGAAGTGGACGGAGTACCGGGTGTGGGTGCGGGCACACACAGACGTGGGCC
  CCGGCCCCGAGAGCAGCCCGGTGCTGGTGCGCACCGATGAGGACGTGCCCAGCGGGCCTC
  CGCGGAAGGTGGAGGTGGAGCCACTGAACTCCACTGCTGTGCATGTCTACTGGAAGCTGC
  CTGTCCCCAGCAAGCAGCATGGCCAGATCCGCGGCTACCAGGTCACCTACGTGCGGCTGG
  AGAATGGCGAGCCCCGTGGACTCCCCATCATCCAAGACGTCATGCTAGCCGAGGCCCAGG
  AAACCACTATCAGCGGCCTGACCCCGGAGACCACCTACTCCGTTACTGTTGCTGCCTATA
  CCACCAAGGGGGATGGTGCCCGCAGCAAGCCCAAAATTGTCACTACAACAGGTGCAGTCC
  CAGGCCGGCCCACCATGATGATCAGCACCACGGCCATGAACACTGCGCTGCTCCAGTGGC
  ACCCACCCAAGGAACTGCCTGGCGAGCTGCTGGGCTACCGGCTGCAGTACTGCCGGGCCG
  ACGAGGCGCGGCCCAACACCATAGATTTCGGCAAGGATGACCAGCACTTCACAGTCACCG
  GCCTGCACAAGGGGACCACCTACATCTTCCGGCTTGCTGCCAAGAACCGGGCTGGCTTGG
  GTGAGGAGTTCGAGAAGGAGATCAGGACCCCCGAGGACCTGCCCAGCGGCTTCCCCCAAA
  ACCTGCATGTGACAGGACTGACCACGTCTACCACAGAACTGGCCTGGGACCCGCCAGTGC
  TGGCGGAGAGGAACGGGCGCATCATCAGCTACACCGTGGTGTTCCGAGACATCAACAGCC
  AACAGGAGCTGCAGAACATCACGACAGACACCCGCTTTACCCTTACTGGCCTCAAGCCAG
  ACACCACTTACGACATCAAGGTCCGCGCATGGACCAGCAAAGGCTCTGGCCCACTCAGCC
  CCAGCATCCAGTCCCGGACCATGCCGGTGGAGCAAGTGTTTGCCAAGAACTTCCGGGTGG
  CGGCTGCAATGAAGACGTCTGTGCTGCTCAGCTGGGAGGTTCCCGACTCCTATAAGTCAG
  CTGTGCCCTTTAAGATTCTGTACAATGGGCAGAGTGTGGAGGTGGACGGGCACTCGATGC
  GGAAGCTGATCGCAGACCTGCAGCCCAACACAGAGTACTCGTTTGTGCTGATGAACCGTG
  GCAGCAGCGCAGGGGGCCTGCAGCACCTGGTGTCCATCCGCACAGCCCCCGACCTCCTGC
  CTCACAAGCCGCTGCCTGCCTCTGCCTACATAGAGGACGGCCGCTTCGATCTCTCCATGC
  CCCATGTGCAAGACCCCTCGCTTGTCAGGTGGTTCTACATTGTTGTGGTGCCCATTGACC
  GTGTGGGCGGGAGCATGCTGACGCCAAGGTGGAGCACACCCGAGGAACTGGAGCTGGACG
  AGCTTCTAGAAGCCATCGAGCAAGGCGGAGAGGAGCAGCGGCGGCGGCGGCGGCAGGCAG
  AACGTCTGAAGCCATATGTGGCTGCTCAACTGGATGTGCTCCCGGAGACCTTTACCTTGG
  GGGACAAGAAGAACTACCGGGGCTTCTACAACCGGCCCCTGTCTCCGGACTTGAGCTACC
  AGTGCTTTGTGCTTGCCTCCTTGAAGGAACCCATGGACCAGAAGCGCTATGCCTCCAGCC
  CCTACTCGGATGAGATCGTGGTCCAGGTGACACCAGCCCAGCAGCAGGAGGAGCCGGAGA
  TGCTGTGGGTGACGGGTCCCGTGCTGGCAGTCATCCTCATCATCCTCATTGTCATCGCCA
  TCCTCTTGTTCAAAAGGAAAAGGACCCACTCTCCGTCCTCTAAGGATGAGCAGTCGATCG
  GACTGAAGGACTCCTTGCTGGCCCACTCCTCTGACCCTGTGGAGATGCGGAGGCTCAACT
  ACCAGACCCCAGGTATGCGAGACCACCCACCCATCCCCATCACCGACCTGGCGGACAACA
  TCGAGCGCCTCAAAGCCAACGATGGCCTCAAGTTCTCCCAGGAGTATGAGTCCATCGACC
  CTGGACAGCAGTTCACGTGGGAGAATTCAAACCTGGAGGTGAACAAGCCCAAGAACCGCT
  ATGCGAATGTCATCGCCTACGACCACTCTCGAGTCATCCTTACCTCTATCGATGGCGTCC
  CCGGGAGTGACTACATCAATGCCAACTACATCGATGGCTACCGCAAGCAGAATGCCTACA
  CCGCCACGCAGGGCCCCCTGCCCGAGACCATGGGTGATTTCTGGAGGATGGTGTGGGAAC
  AGCGCACGGCCACTGTGGTCATGATGACACGGCTGGAGGAGAAGTCCCGGGTAAAATGTG
  ATCAGTACTGGCCAGCCCGTGGCACCGAGACCTGTGGCCTTATTCAGGTGACCCTGTTGG
  ACACAGTGGAGCTGGCCACATACACTGTGCGCACCTTCGCACTCCACAAGAGTGGCTCCA
  GTGAGAAGCGCGAGCTGCGTCAGTTTCAGTTCATGGCCTGGCCAGACCATGGAGTTCCTG
  AGTACCCAACTCCCATCCTGGCCTTCCTACGACGGGTCAAGGCCTGCAACCCCCTAGACG
  CAGGGCCCATGGTGGTGCACTGCAGCGCGGGCGTGGGCCGCACCGGCTGCTTCATCGTGA
  TTGATGCCATGTTGGAGCGGATGAAGCACGAGAAGACGGTGGACATCTATGGCCACGTGA
  CCTGCATGCGATCACAGAGGAACTACATGGTGCAGACGGAGGACCAGTACGTGTTCATCC
  ATGAGGCGCTGCTGGAGGCTGCCACGTGCGGCCACACAGAGGTGCCTGCCCGCAACCTGT
  ATGCCCACATCCAGAAGCTGGGCCAAGTGCCTCCAGGGGAGAGTGTGACCGCCATGGAGC
  TCGAGTTCAAGTTGCTGGCCAGCTCCAAGGCCCACACGTCCCGCTTCATCAGCGCCAACC
  TGCCCTGCAACAAGTTCAAGAACCGGCTGGTGAACATCATGCCCTACGAATTGACCCGTG
  TGTGTCTGCAGCCCATCCGTGGTGTGGAGGGCTCTGACTACATCAATGCCAGCTTCCTGG
  ATGGTTATAGACAGCAGAAGGCCTACATAGCTACACAGGGGCCTCTGGCAGAGAGCACCG
  AGGACTTCTGGCGCATGCTATGGGAGCACAATTCCACCATCATCGTCATGCTGACCAAGC
  TTCGGGAGATGGGCAGGGAGAAATGCCACCAGTACTGGCCAGCAGAGCGCTCTGCTCGCT
  ACCAGTACTTTGTTGTTGACCCGATGGCTGAGTACAACATGCCCCAGTATATCCTGCGTG
  AGTTCAAGGTCACGGATGCCCGGGATGGGCAGTCAAGGACAATCCGGCAGTTCCAGTTCA
  CAGACTGGCCAGAGCAGGGCGTGCCCAAGACAGGCGAGGGATTCATTGACTTCATCGGGC
  AGGTGCATAAGACCAAGGAGCAGTTTGGACAGGATGGGCCTATCACGGTGCACTGCAGTG
  CTGGCGTGGGCCGCACCGGGGTGTTCATCACTCTGAGCATCGTCCTGGAGCGCATGCGCT
  ACGAGGGCGTGGTCGACATGTTTCAGACCGTGAAGACCCTGCGTACACAGCGTCCTGCCA
  TGGTGCAGACAGAGGACCAGTATCAGCTGTGCTACCGTGCGGCCCTGGAGTACCTCGGCA
  GCTTTGACCACTATGCAACGTAACTACCGCTCCCCTCTCCTCCGCCACCCCCGCCGTGGG
  GCTCCGGAGGGGACCCAGCTCCTCTGAGCCATACCGACCATCGTCCAGCCCTCCTACGCA
  GATGCTGTCACTGGCAGAGCACAGCCCACGGGGATCACAGCGTTTCAGGAACGTTGCCAC
  ACCAATCAGAGAGCCTAGAACATCCCTGGGCAAGTGGATGGCCCAGCAGGCAGGCACTGT
  GGCCCTTCTGTCCACCAGACCCACCTGGAGCCCGCTTCAAGCTCTCTGTTGCGCTCCCGC
  ATTTCTCATGCTTCTTCTCATGGGGTGGGGTTGGGGCAAAGCCTCCTTTTTAATACATTA
  AGTGGGGTAGACTGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_130440
• Insert Size: 6400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_130440.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130440.2, NP_569707.2
• RefSeq Size: 7706 bp
• RefSeq ORF: 5697 bp
• Locus ID: 5792
• Cytogenetics: 1p34.2
• Domains: Y_phosphatase, ig, PTPc_motif, IGc2, IG, FN3
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: Adherens junction, Cell adhesion molecules (CAMs), Insulin signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains, and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherents junctions, as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.