N WASP (WASL) (NM_003941) Human Tagged ORF Clone

CAT#: RG207967

WASL (GFP-tagged) - Human Wiskott-Aldrich syndrome-like (WASL)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: WASL
• Synonyms: N-WASP; NWASP; WASPB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207967 representing NM_003941
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCGTCCAGCAGCAGCCGCCGCCGCCGCGGAGGGTCACCAACGTGGGGTCCCTGTTGCTCACCC
  CGCAGGAGAACGAGTCCCTCTTCACTTTCCTCGGCAAGAAATGTGTGACTATGTCTTCAGCAGTGGTGCA
  GTTATATGCAGCAGATCGGAACTGTATGTGGTCAAAGAAGTGCAGTGGTGTTGCTTGTCTTGTTAAGGAC
  AATCCACAGAGATCTTATTTTTTAAGAATATTTGACATTAAGGATGGGAAACTATTGTGGGAACAAGAGC
  TATACAATAACTTTGTATATAATAGTCCTAGAGGATATTTTCATACCTTTGCTGGAGATACTTGTCAAGT
  TGCTCTTAATTTTGCCAATGAAGAAGAAGCAAAAAAATTTCGAAAAGCAGTTACAGACCTTTTGGGCCGT
  CGACAAAGGAAATCTGAGAAAAGACGAGATCCCCCAAATGGTCCTAATCTACCCATGGCTACAGTTGATA
  TAAAAAATCCAGAAATCACAACAAATAGATTTTATGGTCCACAAGTCAACAACATCTCCCATACCAAAGA
  AAAGAAGAAGGGAAAAGCTAAAAAGAAGAGATTAACCAAGGCAGATATAGGAACACCAAGCAATTTCCAG
  CACATTGGACATGTTGGTTGGGATCCAAATACAGGCTTTGATCTGAATAATTTGGATCCAGAATTGAAGA
  ATCTTTTCGATATGTGTGGAATCTCAGAGGCACAACTTAAAGACAGAGAAACATCAAAAGTTATATATGA
  CTTTATTGAAAAAACAGGAGGTGTTGAAGCTGTTAAAAATGAACTGCGGAGGCAAGCACCACCACCTCCA
  CCACCATCAAGGGGAGGGCCACCTCCTCCTCCTCCCCCTCCACACAACTCAGGTCCTCCTCCTCCTCCTG
  CTAGGGGAAGAGGCGCTCCTCCCCCACCACCTTCAAGAGCTCCCACAGCTGCACCTCCACCACCGCCTCC
  TTCCAGGCCAAGTGTAGCAGTCCCTCCACCACCGCCAAATAGGATGTACCCTCCTCCACCTCCAGCCCTT
  CCCTCCTCAGCACCTTCAGGGCCTCCACCACCACCTCCATCTGTGTTGGGGGTAGGGCCAGTGGCACCAC
  CCCCACCGCCTCCACCTCCACCTCCTCCTGGGCCACCGCCCCCGCCTGGCCTGCCTTCTGATGGGGACCA
  TCAGGTTCCAACTACTGCAGGAAACAAAGCAGCTCTTTTAGATCAAATTAGAGAGGGTGCTCAGCTAAAA
  AAAGTGGAGCAGAACAGTCGGCCAGTGTCCTGCTCTGGACGAGATGCACTGTTAGACCAGATACGACAGG
  GTATCCAACTAAAATCTGTGGCTGATGGCCAAGAGTCTACACCACCAACACCTGCACCCACTTCAGGAAT
  TGTGGGTGCATTAATGGAAGTGATGCAGAAAAGGAGCAAAGCCATTCATTCTTCAGATGAAGATGAAGAT
  GAAGATGATGAAGAAGATTTTGAGGATGATGATGAGTGGGAAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207967 representing NM_003941
  Red=Cloning site Green=Tags(s)
  
  MSSVQQQPPPPRRVTNVGSLLLTPQENESLFTFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKD
  NPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGR
  RQRKSEKRRDPPNGPNLPMATVDIKNPEITTNRFYGPQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQ
  HIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPP
  PPSRGGPPPPPPPPHNSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPSVAVPPPPPNRMYPPPPPAL
  PSSAPSGPPPPPPSVLGVGPVAPPPPPPPPPPPGPPPPPGLPSDGDHQVPTTAGNKAALLDQIREGAQLK
  KVEQNSRPVSCSGRDALLDQIRQGIQLKSVADGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDED
  EDDEEDFEDDDEWED
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003941
• ORF Size: 1515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003941.2, NP_003932.3
• RefSeq Size: 4430
• RefSeq ORF: 1518
• Locus ID: 8976
• Domains: PBD, WH1, WH2
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton
• Gene Summary: This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension. [provided by RefSeq, Jul 2013]