VPS4B (NM_004869) Human Tagged ORF Clone

CAT#: RG208019

VPS4B (GFP-tagged) - Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: VPS4B
• Synonyms: MIG1; SKD1; SKD1B; VPS4-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208019 representing NM_004869
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATCCACTTCGCCCAACCTCCAGAAAGCGATAGATCTGGCTAGCAAAGCAGCGCAAGAAGACAAGG
  CTGGGAACTACGAAGAAGCCCTTCAGCTCTATCAGCATGCTGTGCAGTATTTTCTTCATGTCGTTAAATA
  TGAAGCACAGGGTGATAAAGCCAAGCAAAGTATCAGGGCAAAGTGTACAGAATATCTTGATAGAGCAGAA
  AAACTAAAGGAGTACCTGAAAAATAAAGAGAAAAAAGCACAGAAGCCAGTGAAAGAAGGACAGCCGAGTC
  CAGCAGATGAGAAGGGGAATGACAGTGATGGGGAAGGAGAATCTGATGATCCTGAAAAAAAGAAACTACA
  GAATCAACTTCAAGGTGCCATTGTTATAGAACGACCAAATGTGAAATGGAGTGACGTTGCTGGACTTGAA
  GGAGCCAAAGAAGCACTGAAAGAGGCTGTGATACTGCCTATTAAATTTCCTCATCTTTTTACAGGCAAGA
  GAACACCTTGGAGGGGAATCCTATTATTTGGGCCGCCTGGAACAGGAAAGTCCTACTTAGCCAAAGCTGT
  AGCAACAGAAGCCAACAACTCAACATTTTTTTCAATATCTTCCTCTGATCTTGTTTCTAAGTGGCTAGGT
  GAAAGTGAAAAACTGGTTAAGAATTTATTCCAACTTGCCAGAGAGAACAAGCCCTCCATTATCTTCATTG
  ATGAAATTGATTCTCTCTGTGGTTCAAGAAGTGAAAATGAAAGTGAAGCCGCACGTAGAATTAAGACGGA
  GTTCCTAGTGCAAATGCAAGGGGTTGGTGTAGACAATGATGGAATTTTGGTTCTGGGAGCTACAAATATA
  CCCTGGGTTCTGGATTCTGCCATTAGGCGAAGATTTGAGAAACGAATTTATATTCCCTTGCCGGAACCCC
  ATGCCCGAGCAGCAATGTTTAAACTGCACCTAGGGACCACTCAGAACAGTCTCACGGAAGCAGACTTTCG
  GGAACTTGGGAGGAAAACAGATGGTTATTCAGGGGCAGATATAAGTATCATTGTACGTGATGCCCTTATG
  CAGCCTGTTAGGAAAGTACAGTCAGCTACTCATTTTAAAAAGGTTCGCGGACCTTCCCGAGCTGATCCTA
  ACCATCTTGTAGATGATCTGCTAACACCTTGCTCTCCAGGTGACCCTGGTGCCATTGAAATGACATGGAT
  GGATGTCCCTGGAGATAAACTTTTGGAGCCAGTTGTTTCCATGTCGGATATGTTGCGGTCACTATCTAAC
  ACAAAACCTACAGTCAATGAACATGACTTGTTGAAATTAAAGAAGTTTACAGAAGATTTTGGTCAAGAAG
  GC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208019 representing NM_004869
  Red=Cloning site Green=Tags(s)
  
  MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAE
  KLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLE
  GAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLG
  ESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNI
  PWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALM
  QPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSN
  TKPTVNEHDLLKLKKFTEDFGQEG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004869
• ORF Size: 1332 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004869.3, NP_004860.2
• RefSeq Size: 3396
• RefSeq ORF: 1335
• Locus ID: 9525
• Domains: AAA, AAA, MIT
• Protein Families: Transcription Factors
• Protein Pathways: Endocytosis
• Gene Summary: The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq, Jul 2008]