VPS4B (NM_004869) Human Untagged Clone

CAT#: SC127708

VPS4B (untagged)-Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VPS4B
• Synonyms: MIG1; SKD1; SKD1B; VPS4-2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127708 sequence for NM_004869 edited (data generated by NextGen Sequencing)
  ATGTCATCCACTTCGCCCAACCTCCAGAAAGCGATAGATCTGGCTAGCAAAGCAGCGCAA
  GAAGACAAGGCTGGGAACTACGAAGAAGCCCTTCAGCTCTATCAGCATGCTGTGCAGTAT
  TTTCTTCATGTCGTTAAATATGAAGCACAGGGTGATAAAGCCAAGCAAAGTATCAGGGCA
  AAGTGTACAGAATATCTTGATAGAGCAGAAAAACTAAAGGAGTACCTGAAAAATAAAGAG
  AAAAAAGCACAGAAGCCAGTGAAAGAAGGACAGCCGAGTCCAGCAGATGAGAAGGGGAAT
  GACAGTGATGGGGAAGGAGAATCTGATGATCCTGAAAAAAAGAAACTACAGAATCAACTT
  CAAGGTGCCATTGTTATAGAACGACCAAATGTGAAATGGAGTGACGTTGCTGGACTTGAA
  GGAGCCAAAGAAGCACTGAAAGAGGCTGTGATACTGCCTATTAAATTTCCTCATCTTTTT
  ACAGGCAAGAGAACACCTTGGAGGGGAATCCTATTATTTGGGCCGCCTGGAACAGGAAAG
  TCCTACTTAGCCAAAGCTGTAGCAACAGAAGCCAACAACTCAACATTTTTTTCAATATCT
  TCCTCTGATCTTGTTTCTAAGTGGCTAGGTGAAAGTGAAAAACTGGTTAAGAATTTATTC
  CAACTTGCCAGAGAGAACAAGCCCTCCATTATCTTCATTGATGAAATTGATTCTCTCTGT
  GGTTCAAGAAGTGAAAATGAAAGTGAAGCCGCACGTAGAATTAAGACGGAGTTCCTAGTG
  CAAATGCAAGGGGTTGGTGTAGACAATGATGGAATTTTGGTTCTGGGAGCTACAAATATA
  CCCTGGGTTCTGGATTCTGCCATTAGGCGAAGATTTGAGAAACGAATTTATATTCCCTTG
  CCGGAACCCCATGCCCGAGCAGCAATGTTTAAACTGCACCTAGGGACCACTCAGAACAGT
  CTCACGGAAGCAGACTTTCGGGAACTTGGGAGGAAAACAGATGGTTATTCAGGGGCAGAT
  ATAAGTATCATTGTACGTGATGCCCTTATGCAGCCTGTTAGGAAAGTACAGTCAGCTACT
  CATTTTAAAAAGGTTCGCGGACCTTCCCGAGCTGATCCTAACCATCTTGTAGATGATCTG
  CTAACACCTTGCTCTCCAGGTGACCCTGGTGCCATTGAAATGACATGGATGGATGTCCCT
  GGAGATAAACTTTTGGAGCCAGTTGTTTCCATGTCGGATATGTTGCGGTCACTATCTAAC
  ACAAAACCTACAGTCAATGAACATGACTTGTTGAAATTAAAGAAGTTTACAGAAGATTTT
  GGTCAAGAAGGCTAA
  
  Clone variation with respect to NM_004869.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004869 unedited
  CGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGTCCGAGAACCACCGGCCTC
  CCCAGTTTGAGGGCTGTTACCCCGTGCGCGCTTCGACGTTGCTGCTGTTGGCTCTCCTCG
  CCCCTCGTTCCCTTGGGAACCGCCTGGGAACTCCGCCATGTCATCCACTTCGCCCAACCT
  CCAGAAAGCGATAGATCTGGCTAGCAAAGCAGCGCAAGAAGACAAGGCTGGGAACTACGA
  AGAAGCCCTTCAGCTCTATCAGCATGCTGTGCAGTATTTTCTTCATGTCGTTAAATATGA
  AGCACAGGGTGATAAAGCCAAGCAAAGTATCAGGGCAAAGTGTACAGAATATCTTGATAG
  AGCAGAAAAACTAAAGGAGTACCTGAAAAATAAAGAGAAAAAAGCACAGAAGCCAGTGAA
  AGAAGGACAGCCGAGTCCAGCAGATGAGAAGGGGAATGACAGTGATGGGGAAGGAGAATC
  TGATGATCCTGANAAAAAGAAACTACAGAATCAACTTCAAGGTGCCATTGTTATAGAACG
  ACCAAATGTGAAATGGAGTGACGTTGCTGGACTTGAAGGAGCCAAAGAAGCACTGGAAGA
  GGCTGTGATACTGCCTATTAATTNCCTCATCTTTNTACAGGGCAGAGAACACCTTGGNAG
  GGGATCCCNTATATTTGGCCGCCTGGAACAGGNAAGTCCTACTTAGCCAAAGCTGTAGCA
  ACAGAAGCCAANCCACTCACATTTTTTACAATATCTCCTCTGGATCTGNTNCTAAGTGGC
  TAGTGAAAGTGAAAAACTGGGTAAGAANTTATTNCNNACTGCCAGAGAGAACCAGCCCTC
  CATTATCTNCATTGATGAAAANTGATCTTCCTGTGGNTCAAGAAAGTGAAATGAAAGTGA
  GCCCCACGTANAATTAGACGGAGTCCTATGCCATGCAGGGGTNGNGTANCATGATGAATT
  TGGNTCTGGACTACAATTACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004869 unedited
  GCGGCCGCGGGGGCAGNCCGCANACTCTCCCNNNNGGTTTTTACTTGNNACGCGGCCGCT
  TTTNANATCGNGTTTTTTTTTTTTTTTTTTTTTAGGGTATATCAATTTACTTCACATAAG
  AGCATAATTCAGGCCTTTTCCATTTAATAACAAAAACAATCTGTACAGAAAACCCAAAGG
  CAACCACATAGCATATGTAAAATGTGCAAATACACTTTAAAATGCAAGTTATTCTATAGC
  ATTTGCAAGATAGAATTTCACTGTAATTAGGGAATCTAGTTCATCCTAACTTAATAGTCT
  TTTGCATGTATAGACAATGCAATTCTACAAGGCACAACTCAGCGTTGATGCTAAAGTATG
  AAACACATCCTCAGATTATTTATTTGAAAATATTAAAATAGCATCATGTTTATTATTTTT
  TAATGAGTCATGAGCTCATTTCTAAAGCTTCATAAAGCATTACACTGATAACATATGTGT
  GGTCAGGACAAACTGTTCCCTGAACTTAAAAGGTGAAGGACAAGACCCCATATTATTATC
  CTGTATTAAAAAAGGAAATATACATATATGTACACAGACACCCCATATCACAGACAAGAA
  ACTTCCCATAATTCAAAGGGAGACCATTTCCTTATTAGCAAAGGTGCGCATTACAGTATT
  TCATGACAGTTAAAAATTACACACCTACCACCTGTTTTTGGTCAATCTTGCTAAAAAAGA
  ACTGAAATAGACAATTTCTTCTTTAAGGTAAAGACAATGTCTAATTTAGAAAATCTTCCT
  CTTGAGATGCACAGTGAAAGGTATCAGNTAATTAATATTAAAGCTTACCGTTTTTGTTTT
  TTTTGAGACGAGTTTTATTCAGTCGCCAAGCTTGATGCAATGGGCTTCTTGGGCCCAAGC
  TGGATGCAATACGCGATTCAACTACTGGAACTCTTGCTTCAGTTCAGTGATTCTCCGCT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004869
• ORF Size: 1335 bp
• Insert Size: 3160
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_004869.2, NP_004860.2
• RefSeq Size: 3337
• RefSeq ORF: 1335
• Locus ID: 9525
• Domains: AAA, AAA, MIT
• Protein Families: Transcription Factors
• Protein Pathways: Endocytosis
• Gene Summary: The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq, Jul 2008]