VPS4B (NM_004869) Human Untagged Clone

CAT#: SC324083

VPS4B (untagged)-Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VPS4B
• Synonyms: MIG1; SKD1; SKD1B; VPS4-2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004869.3 GGAGAGAGTGTTGTCTAAAACAAGTTCCGGAAGGGAGGCTGCCCTTCGCGGTCCGAGAAC
  CACCGGCCTCCCCAGTTTGAGGGCTGTTACCCCGTGCGCGCTTCGACGTTGCTGCTGTTG
  GCTCTCCTCGCCCCTCGTTCCCTTGGGAACCGCCTGGGAACTCCGCCATGTCATCCACTT
  CGCCCAACCTCCAGAAAGCGATAGATCTGGCTAGCAAAGCAGCGCAAGAAGACAAGGCTG
  GGAACTACGAAGAAGCCCTTCAGCTCTATCAGCATGCTGTGCAGTATTTTCTTCATGTCG
  TTAAATATGAAGCACAGGGTGATAAAGCCAAGCAAAGTATCAGGGCAAAGTGTACAGAAT
  ATCTTGATAGAGCAGAAAAACTAAAGGAGTACCTGAAAAATAAAGAGAAAAAAGCACAGA
  AGCCAGTGAAAGAAGGACAGCCGAGTCCAGCAGATGAGAAGGGGAATGACAGTGATGGGG
  AAGGAGAATCTGATGATCCTGAAAAAAAGAAACTACAGAATCAACTTCAAGGTGCCATTG
  TTATAGAACGACCAAATGTGAAATGGAGTGACGTTGCTGGACTTGAAGGAGCCAAAGAAG
  CACTGAAAGAGGCTGTGATACTGCCTATTAAATTTCCTCATCTTTTTACAGGCAAGAGAA
  CACCTTGGAGGGGAATCCTATTATTTGGGCCGCCTGGAACAGGAAAGTCCTACTTAGCCA
  AAGCTGTAGCAACAGAAGCCAACAACTCAACATTTTTTTCAATATCTTCCTCTGATCTTG
  TTTCTAAGTGGCTAGGTGAAAGTGAAAAACTGGTTAAGAATTTATTCCAACTTGCCAGAG
  AGAACAAGCCCTCCATTATCTTCATTGATGAAATTGATTCTCTCTGTGGTTCAAGAAGTG
  AAAATGAAAGTGAAGCCGCACGTAGAATTAAGACGGAGTTCCTAGTGCAAATGCAAGGGG
  TTGGTGTAGACAATGATGGAATTTTGGTTCTGGGAGCTACAAATATACCCTGGGTTCTGG
  ATTCTGCCATTAGGCGAAGATTTGAGAAACGAATTTATATTCCCTTGCCGGAACCCCATG
  CCCGAGCAGCAATGTTTAAACTGCACCTAGGGACCACTCAGAACAGTCTCACGGAAGCAG
  ACTTTCGGGAACTTGGGAGGAAAACAGATGGTTATTCAGGGGCAGATATAAGTATCATTG
  TACGTGATGCCCTTATGCAGCCTGTTAGGAAAGTACAGTCAGCTACTCATTTTAAAAAGG
  TTCGCGGACCTTCCCGAGCTGATCCTAACCATCTTGTAGATGATCTGCTAACACCTTGCT
  CTCCAGGTGACCCTGGTGCCATTGAAATGACATGGATGGATGTCCCTGGAGATAAACTTT
  TGGAGCCAGTTGTTTCCATGTCGGATATGTTGCGGTCACTATCTAACACAAAACCTACAG
  TCAATGAACATGACTTGTTGAAATTAAAGAAGTTTACAGAAGATTTTGGTCAAGAAGGCT
  AAGCCAAAGACAAGGAAGATGCTTACCATATGTATTCTTTCTTTCATAGATATTTTTGTC
  TATTTGGATCGCATTAATTGTTTCCAGTAAAACTCTTTTACCACAGGGAAATACACATCT
  CACTTCAGAGTTCCATTAGGTTTTATATTGTACTTTTCCTCCATTACTTATTAAATACTC
  CTATTAACAAAAGGTACAAAATAACAGGTTATGAGGAAATGAGCGATATATGAACGGCAT
  AAAAACAGAAATTACCCAGTAAAAAGGATGTCAGAAATTGACATACAAATATTTACAATT
  TTTATGAATGGTGGTCTTTGCAAAGAGCATTTATATTTTCTTTTTTTTTTACTAAAATGA
  TATAGGGTTTATTTTATATTTTCAAAAAAATTGTTAAACATCATTCTTATCAATGTAAAA
  TTTACGTTATTAAAAAATTACAAATGATAGAATCTTTACTCAGGGATGGGCAATAAAACA
  GCAAAGAGCTTTGTGATTGGGTTTGAAAGATTTTGAATTATAGACAGTGCTCTTAATAGT
  TTTTAATAAGTTGATATTTTTTTCTGTGTAGATTTAAATAATTTCTTTAAAAGCGTAAGC
  TTTGGCTGGGCATGGTGGCTTATGCCTGTAATCCCAGCACTTTGGGAGGCTACGATGGGT
  GGATCACCTGAGGTCAGGAGTTCAAGACCAGTCTGGCCAACATGGTGAAATGCCGTCTCT
  ACTAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004869.3 unedited
  GGCGTTTGTAATACGACTCCTATAGGGCGGCCGGCGATCGCGGCGCGCCGGTACCGGTCC
  GGAATTCCCGGGATATCGTCGACCCACGCGTCCGGGAGAGAGTGTTGTCTAAAACAAGTT
  CCGGAAGGGAGGCTGCCCTTCGCGGTCCGAGAACCACCGGCCTCCCCAGTTTGAGGGCTG
  TTACCCCGTGCGCGCTTCGACGTTGCTGCTGTTGGCTCTCCTCGCCCCTCGTTCCCTTGG
  GAACCGCCTGGGAACTCCGCCATGTCATCCACTTCGCCCAACCTCCAGAAAGCGATAGAT
  CTGGCTAGCAAAGCAGCGCAAGAAGACAAGGCTGGGAACTACGAAGAAGCCCTTCAGCTC
  TATCAGCATGCTGTGCAGTATTTTCTTCATGTCGTTAAATATGAAGCACAGGGTGATAAA
  GCCAAGCAAAGTATCAGGGCAAAGTGTACAGAATATCTTGATAGAGCAGAAAAACTAAAG
  GAGTACCTGAAAAATAAAGAGAAAAAAGCACAGAAGCCAGTGAAAGAAGGACAGCCGAGT
  CCAGCAGATGAGAAGGGGAATGACAGTGATGGGGAAGGAGAATCTGATGATCCTGAAAAA
  AAGAAACTACAGAATCAACTTCAAGGTGCCATTGTTATAGAACGACCAAATGTGAAATGG
  AGTGACGTTGCTGGACTTGAAGGAGCCAAAGAAGCACTGAAAGAGGCTGTGATACTGCCT
  ATTAAATTTCCTCATCTTTTTACAGGCAAGAGACACCTTGGAGGGGAATCCTATTATTTG
  GGCCCGCCTGAACAGGAAAGTCCTACTTAGCCAAAGCTGTAGCACAGAGCCAACACTCAA
  CATTTTTTTCAATATCTCTCTGATCTTGTTTCTAAGTGGCTAGTGAAAGTGAAAACCTGC
  TAGATTTATCACTGCCAGAGAGAACAAAGCCCTCCATTATCTTCATTGGAGTGAATGGA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004869
• ORF Size: 1335 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_004869.3, NP_004860.2
• RefSeq Size: 3396
• RefSeq ORF: 1335
• Locus ID: 9525
• Domains: AAA, AAA, MIT
• Protein Families: Transcription Factors
• Protein Pathways: Endocytosis
• Gene Summary: The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq, Jul 2008]