ARHGEF6 (NM_004840) Human Tagged ORF Clone

CAT#: RG208074

ARHGEF6 (GFP-tagged) - Human Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 (ARHGEF6)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ARHGEF6
• Synonyms: alpha-PIX; alphaPIX; Cool-2; COOL2; MRX46; PIXA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208074 representing NM_004840
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCCAGAAGAACAAATCGTGACATGGCTTATATCTTTGGGAGTTTTAGAGTCCCCTAAAAAGACCA
  TCTGTGATCCGGAGGAGTTTTTAAAGTCCTCGCTGAAAAATGGGGTAGTTCTGTGCAAACTGATCAACAG
  ACTCATGCCTGGCTCTGTGGAAAAGTTTTGTCTGGATCCCCAAACTGAAGCTGACTGCATCAACAACATC
  AATGACTTCCTGAAAGGATGTGCAACCCTCCAAGTGGAAATATTTGATCCTGATGACCTTTATTCAGGGG
  TCAATTTCTCCAAGGTACTGAGTACTCTTTTAGCTGTCAACAAAGCAACAGAAGATCAGCTATCAGAAAG
  ACCATGTGGACGTTCCTCTTCTCTTAGTGCTGCTAATACTTCTCAGACAAACCCACAGGGAGCAGTTTCT
  AGCACAGTTTCAGGGCTGCAAAGGCAGTCAAAGACAGTGGAGATGACGGAAAATGGAAGTCATCAGTTGA
  TAGTAAAAGCAAGATTCAACTTTAAGCAGACTAATGAGGATGAACTGTCAGTTTGTAAGGGGGACATCAT
  TTACGTCACACGAGTTGAAGAAGGAGGCTGGTGGGAAGGCACATTAAATGGGAGAACAGGCTGGTTCCCC
  AGTAATTATGTCCGTGAAATTAAATCCAGTGAGAGACCTCTCTCCCCAAAAGCCGTCAAAGGATTTGAAA
  CTGCTCCACTTACCAAGAATTATTATACTGTGGTGTTACAGAACATCCTGGACACTGAAAAAGAATATGC
  TAAAGAACTTCAGTCTCTTCTTGTTACTTACTTAAGACCCCTGCAGTCCAATAACAATCTGAGTACTGTG
  GAGGTTACATCTTTACTGGGAAACTTCGAGGAAGTATGCACATTTCAACAGACACTCTGCCAAGCCTTGG
  AAGAATGTTCAAAGTTTCCAGAAAACCAGCACAAAGTAGGAGGTTGTCTACTGAGTCTCATGCCTCATTT
  TAAATCTATGTATCTGGCTTACTGTGCAAACCATCCTTCAGCTGTAAATGTGCTCACTCAGCACAGTGAT
  GAGTTGGAACAATTCATGGAAAATCAAGGTGCATCGAGCCCAGGTATCCTCATTTTAACAACAAACCTCA
  GCAAACCATTCATGCGACTGGAGAAATATGTTACTCTCTTGCAAGAGTTAGAACGGCATATGGAGGATAC
  TCATCCAGATCATCAGGATATTCTGAAAGCAATCGTAGCATTCAAAACTCTCATGGGGCAATGTCAAGAT
  CTGAGGAAGAGAAAACAGCTGGAGTTACAGATACTGTCCGAACCTATTCAGGCATGGGAAGGAGAAGATA
  TTAAAAACTTGGGAAATGTGATTTTTATGTCACAAGTAATGGTGCAGTATGGAGCATGTGAGGAAAAAGA
  GGAGCGGTACCTTATGTTATTTTCAAATGTCCTGATAATGTTATCTGCAAGTCCTCGGATGAGTGGCTTT
  ATCTATCAGGGAAAAATACCAATAGCAGGAACGGTGGTGACTAGATTAGATGAAATTGAAGGGAATGACT
  GCACATTTGAAATCACTGGTAACACAGTGGAGAGAATTGTGGTCCATTGTAACAACAACCAGGACTTCCA
  GGAATGGTTGGAGCAGCTGAACAGACTGATCAGAGGACCTGCCTCTTGCAGTTCATTATCCAAAACCTCA
  TCGTCATCATGTAGTGCTCATTCTTCTTTTAGCTCTACCGGACAGCCCCGAGGACCCTTGGAGCCTCCTC
  AAATTATAAAACCGTGGAGTTTAAGTTGTCTACGACCTGCACCTCCACTTAGACCATCAGCAGCACTAGG
  TTATAAAGAGAGGATGTCTTATATCTTAAAGGAGTCTAGTAAAAGCCCTAAAACGATGAAGAAATTTCTT
  CATAAAAGGAAGACTGAGAGAAAACCATCGGAGGAGGAATATGTGATTAGGAAAAGTACAGCTGCTCTGG
  AAGAGGATGCTCAAATCCTTAAAGTGATCGAAGCCTACTGCACCAGCGCAAATTTTCAACAAGGCCATGG
  CTCAAGTACTCGAAAAGATTCCATTCCACAAGTCCTACTCCCTGAGGAAGAGAAACTCATCATTGAAGAA
  ACCAGAAGCAACGGCCAGACCATCATGGAAGAAAAGAGCCTTGTTGATACTGTTTACGCCTTGAAGGACG
  AGGTCAGAGAACTGAAGCAGGAAAATAAAAGAATGAAGCAATGCCTGGAAGAAGAACTGAAATCAAGAAG
  GGACCTAGAAAAGCTGGTGCGGAGGCTTTTGAAGCAAACAGATGAGTGTATTCGAGGCGAGTCCAGTAGC
  AAGACCTCAATTCTTCCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208074 representing NM_004840
  Red=Cloning site Green=Tags(s)
  
  MNPEEQIVTWLISLGVLESPKKTICDPEEFLKSSLKNGVVLCKLINRLMPGSVEKFCLDPQTEADCINNI
  NDFLKGCATLQVEIFDPDDLYSGVNFSKVLSTLLAVNKATEDQLSERPCGRSSSLSAANTSQTNPQGAVS
  STVSGLQRQSKTVEMTENGSHQLIVKARFNFKQTNEDELSVCKGDIIYVTRVEEGGWWEGTLNGRTGWFP
  SNYVREIKSSERPLSPKAVKGFETAPLTKNYYTVVLQNILDTEKEYAKELQSLLVTYLRPLQSNNNLSTV
  EVTSLLGNFEEVCTFQQTLCQALEECSKFPENQHKVGGCLLSLMPHFKSMYLAYCANHPSAVNVLTQHSD
  ELEQFMENQGASSPGILILTTNLSKPFMRLEKYVTLLQELERHMEDTHPDHQDILKAIVAFKTLMGQCQD
  LRKRKQLELQILSEPIQAWEGEDIKNLGNVIFMSQVMVQYGACEEKEERYLMLFSNVLIMLSASPRMSGF
  IYQGKIPIAGTVVTRLDEIEGNDCTFEITGNTVERIVVHCNNNQDFQEWLEQLNRLIRGPASCSSLSKTS
  SSSCSAHSSFSSTGQPRGPLEPPQIIKPWSLSCLRPAPPLRPSAALGYKERMSYILKESSKSPKTMKKFL
  HKRKTERKPSEEEYVIRKSTAALEEDAQILKVIEAYCTSANFQQGHGSSTRKDSIPQVLLPEEEKLIIEE
  TRSNGQTIMEEKSLVDTVYALKDEVRELKQENKRMKQCLEEELKSRRDLEKLVRRLLKQTDECIRGESSS
  KTSILP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004840
• ORF Size: 2328 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004840.2, NP_004831.1
• RefSeq Size: 5282
• RefSeq ORF: 2331
• Locus ID: 9459
• Domains: RhoGEF, SH3, CH, PH
• Protein Families: Druggable Genome
• Protein Pathways: Pancreatic cancer, Regulation of actin cytoskeleton
• Gene Summary: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It may form a complex with G proteins and stimulate Rho-dependent signals. This protein is activated by PI3-kinase. Mutations in this gene can cause X-chromosomal non-specific cognitive disability. [provided by RefSeq, Jul 2008]