GRF2 (RAPGEF1) (NM_198679) Human Tagged ORF Clone

CAT#: RG208092

RAPGEF1 (GFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RAPGEF1
• Synonyms: C3G; GRF2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208092 representing NM_198679
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAATGCTATTGAAAAACAGAAGCCCTTGAAACGAAGTCATCTGTACCCCTGGAAACAAGACTCTC
  AGCGTTCTCATCTCTCTTCCTTCACCATGAAGCTGATGGACAAATTCCACTCACCCAAAATCAAGAGAAC
  GCCATCAAAGAAGGGAAAACCAGCTGAGGTGTCCGTAAAGATTCCAGAGAAGCCTGTGAACAAAGAGGCA
  ACAGACAGATTTCTACCAGAGGGCTACCCTCTCCCCTTGGATCTGGAGCAGCAGGCAGTAGAATTTATGT
  CCACCAGTGCTGTGGCTTCCAGGTCTCAAAGGCAGAAGAACCTGAGCTGGCTGGAGGAGAAAGAGAAGGA
  AGTTGTCAGTGCCCTGCGCTACTTTAAGACCATTGTGGACAAAATGGCAATTGATAAGAAGGTACTGGAG
  ATGCTTCCAGGGTCAGCCAGCAAGGTGCTGGAGGCCATCTTACCCCTGGTGCAGAACGATCCTCGAATTC
  AGCACAGCTCAGCCCTCTCTTCCTGCTATAGCCGAGTGTACCAAAGCCTCGCCAACCTCATTCGCTGGTC
  TGACCAAGTGATGCTGGAAGGCGTGAACTCAGAAGACAAGGAGATGGTGACGACTGTGAAGGGGGTCATC
  AAGGCTGTGCTGGATGGAGTGAAGGAGCTGGTCAGGCTCACCATCGAGAAGCAGGGACGTCCGTCTCCGA
  CGAGCCCCGTGAAGCCCAGTTCCCCTGCCAGCAAGCCTGATGGCCCAGCAGAGCTCCCCCTGACAGACCG
  CGAGGTAGAGATCCTAAACAAGACGACTGGGATGTCACAGTCAACTGAGCTCCTCCCAGATGCCACGGAT
  GAAGAGGTCGCGCCCCCCAAGCCTCCTCTGCCTGGCATTCGGGTGGTTGATAATAGTCCTCCACCAGCAT
  TGCCACCCAAGAAAAGACAGTCGGCGCCGTCCCCTACCCGAGTGGCTGTGGTGGCCCCCATGAGCCGAGC
  CACCAGTGGCTCCAGTTTGCCTGTTGGAATCAATAGGCAGGATTTTGATGTTGACTGTTACGCACAGAGG
  CGACTGTCAGGAGGCAGCCACTCATATGGTGGAGAGTCGCCCCGCCTCTCCCCCTGCAGCAGCATAGGCA
  AGCTCAGCAAGTCAGACGAGCAGCTGTCCTCTCTGGACAGGGACAGTGGGCAGTGCTCCCGGAACACAAG
  CTGTGAAACACTAGACCACTATGATCCCGACTATGAATTCCTCCAGCAAGACCTCTCTAACGCAGACCAG
  ATACCTCAGCAGACGGCCTGGAACCTTAGCCCGTTGCCAGAGTCTTTGGGGGAGTCTGGGTCTCCATTTC
  TTGGCCCTCCTTTCCAGCTGCCTCTTGGCGGCCATCCCCAGCCAGACGGACCTCTGGCCCCAGGGCAGCA
  GACAGATACGCCACCTGCTCTCCCCGAGAAGAAGCGCAGGAGCGCAGCCTCCCAGACGGCGGACGGCTCT
  GGCTGCAGGGTGTCCTACGAGCGGCATCCCTCGCAGTATGACAACATCTCTGGGGAGGACCTGCAGAGCA
  CAGCCCCGATCCCATCCGTCCCCTACGCGCCCTTTGCTGCTATTCTGCCCTTTCAGCATGGAGGTTCCTC
  AGCCCCTGTCGAATTTGTGGGTGATTTTACTGCTCCTGAGTCAACCGGTGACCCAGAAAAACCACCTCCT
  CTACCAGAGAAGAAAAACAAACACATGCTGGCCTACATGCAGTTGCTGGAGGACTACTCGGAGCCGCAGC
  CCTCTATGTTCTACCAGACGCCACAGAACGAGCACATCTACCAGCAGAAGAACAAGCTCCTCATGGAGGT
  ATACGGCTTCAGCGACTCCTTCAGTGGGGTGGACTCCGTGCAGGAGCTGGCCCCGCCGCCCGCCCTACCC
  CCCAAGCAGCGGCAGCTGGAGCCACCGGCTGGGAAAGACGGACATCCCAGAGATCCCTCAGCGGTCAGCG
  GCGTCCCTGGGAAGGACAGCAGAGACGGCAGTGAGAGGGCCCCAAAGTCACCAGATGCTCTGGAGTCGGC
  TCAGTCGGAGGAGGAAGTGGACGAGCTGTCCCTCATTGACCACAACGAAATTATGTCCAGGCTGACGCTC
  AAGCAGGAGGGTGATGACGGGCCGGACGTCCGCGGAGGATCTGGGGACATCTTACTGGTCCATGCTACTG
  AGACTGACAGGAAAGATTTGGTGTTGTACTGCGAGGCATTCCTGACCACCTACAGGACCTTCATCTCCCC
  AGAGGAGCTCATCAAGAAGCTGCAGTACAGATATGAGAAATTCTCTCCCTTTGCCGACACATTCAAGAAG
  CGCGTCAGCAAGAACACGTTCTTCGTGCTGGTACGGGTGGTGGATGAGCTCTGCCTGGTGGAGTTGACAG
  AAGAGATCCTGAAGCTGCTGATGGAACTGGTCTTCCGCCTGGTGTGCAATGGGGAGCTGAGCCTGGCCCG
  TGTGCTCCGGAAGAACATCCTGGACAAGGTGGACCAGAAGAAGCTACTCAGGTGTGCCACCTCCAGCCAG
  CCCCTGGCAGCCCGGGGGGTAGCAGCCAGGCCGGGGACCTTGCACGACTTTCACAGCCATGAGATAGCGG
  AGCAGCTAACGCTGCTGGATGCTGAGCTCTTCTATAAAATAGAGATTCCTGAGGTTTTGCTTTGGGCAAA
  AGAGCAGAATGAGGAGAAGAGCCCCAACTTGACCCAGTTCACGGAGCACTTCAACAACATGTCCTACTGG
  GTCCGGTCCATAATCATGTTACAGGAAAAGGCCCAGGACAGGGAACGGCTGCTCTTGAAGTTCATCAAGA
  TCATGAAGCACTTGCGGAAGCTGAATAACTTCAACTCCTACTTGGCCATCCTCTCTGCCCTGGACTCGGC
  GCCCATCCGCAGGCTGGAGTGGCAGAAGCAGACTTCAGAGGGCCTGGCCGAGTACTGCACACTGATCGAC
  AGCTCGTCCTCCTTCCGAGCCTACCGGGCCGCCCTCTCGGAGGTGGAACCGCCGTGCATCCCGTACCTGG
  GGCTGATCCTGCAGGACCTGACCTTCGTTCACCTGGGAAACCCAGACTACATCGACGGGAAAGTGAACTT
  CTCCAAGCGGTGGCAGCAGTTCAACATCCTCGACAGCATGCGCTGCTTCCAGCAGGCGCACTATGACATG
  CGGAGGAACGACGACATTATAAACTTCTTCAATGACTTCAGTGACCACCTGGCTGAGGAGGCCCTATGGG
  AACTGTCTCTGAAAATTAAACCCAGGAACATAACAAGGAGAAAAACAGACCGGGAAGAGAAGACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208092 representing NM_198679
  Red=Cloning site Green=Tags(s)
  
  MGNAIEKQKPLKRSHLYPWKQDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEA
  TDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLE
  MLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVI
  KAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATD
  EEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQR
  RLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQ
  IPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGS
  GCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPP
  LPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALP
  PKQRQLEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTL
  KQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKK
  RVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQ
  PLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYW
  VRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLID
  SSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDM
  RRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198679
• ORF Size: 3285 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198679.1, NP_941372.1
• RefSeq Size: 6204 bp
• RefSeq ORF: 3288 bp
• Locus ID: 2889
• Cytogenetics: 9q34.13
• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, Insulin signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma
• Gene Summary: 'This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]'