GRF2 (RAPGEF1) (NM_198679) Human Untagged Clone

CAT#: SC307763

RAPGEF1 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAPGEF1
• Synonyms: C3G; GRF2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_198679 edited
  CTGGGGCTGGGAAGGGCCCTGTGTCCTCTGGCTTTAAAATGGGCAATGCTATTGAAAAAC
  AGAAGCCCTTGAAACGAAGTCATCTGTACCCCTGGAAACAAGACTCTCAGCGTTCTCATC
  TCTCTTCCTTCACCATGAAGCTGATGGACAAATTCCACTCACCCAAAATCAAGAGAACGC
  CATCAAAGAAGGGAAAACCAGCTGAGGTGTCCGTAAAGATTCCAGAGAAGCCTGTGAACA
  AAGAGGCAACAGACAGATTTCTACCAGAGGGCTACCCTCTCCCCTTGGATCTGGAGCAGC
  AGGCAGTAGAATTTATGTCCACCAGTGCTGTGGCTTCCAGGTCTCAAAGGCAGAAGAACC
  TGAGCTGGCTGGAGGAGAAAGAGAAGGAAGTTGTCAGTGCCCTGCGCTACTTTAAGACCA
  TTGTGGACAAAATGGCAATTGATAAGAAGGTACTGGAGATGCTTCCAGGGTCAGCCAGCA
  AGGTGCTGGAGGCCATCTTACCCCTGGTGCAGAACGATCCTCGAATTCAGCACAGCTCAG
  CCCTCTCTTCCTGCTATAGCCGAGTGTACCAAAGCCTCGCCAACCTCATTCGCTGGTCTG
  ACCAAGTGATGCTGGAAGGCGTGAACTCAGAAGACAAGGAGATGGTGACGACTGTGAAGG
  GGGTCATCAAGGCTGTGCTGGATGGAGTGAAGGAGCTGGTCAGGCTCACCATCGAGAAGC
  AGGGACGTCCGTCTCCGACGAGCCCCGTGAAGCCCAGTTCCCCTGCCAGCAAGCCTGATG
  GCCCAGCAGAGCTCCCCCTGACAGACCGCGAGGTAGAGATCCTAAACAAGACGACTGGGA
  TGTCACAGTCAACTGAGCTCCTCCCAGATGCCACGGATGAAGAGGTCGCGCCCCCCAAGC
  CTCCTCTGCCTGGCATTCGGGTGGTTGATAATAGTCCTCCACCAGCATTGCCACCCAAGA
  AAAGACAGTCGGCGCCGTCCCCTACCCGAGTGGCTGTGGTGGCCCCCATGAGCCGAGCCA
  CCAGTGGCTCCAGTTTGCCTGTTGGAATCAATAGGCAGGATTTTGATGTTGACTGTTACG
  CACAGAGGCGACTGTCAGGAGGCAGCCACTCATATGGTGGAGAGTCGCCCCGCCTCTCCC
  CCTGCAGCAGCATAGGCAAGCTCAGCAAGTCAGACGAGCAGCTGTCCTCTCTGGACAGGG
  ACAGTGGGCAGTGCTCCCGGAACACAAGCTGTGAAACACTAGACCACTATGATCCCGACT
  ATGAATTCCTCCAGCAAGACCTCTCTAACGCAGACCAGATACCTCAGCAGACGGCCTGGA
  ACCTTAGCCCGTTGCCAGAGTCTTTGGGGGAGTCTGGGTCTCCATTTCTTGGCCCTCCTT
  TCCAGCTGCCTCTTGGCGGCCATCCCCAGCCAGACGGACCTCTGGCCCCAGGGCAGCAGA
  CAGATACGCCACCTGCTCTCCCCGAGAAGAAGCGCAGGAGCGCAGCCTCCCAGACGGCGG
  ACGGCTCTGGCTGCAGGGTGTCCTACGAGCGGCATCCCTCGCAGTATGACAACATCTCTG
  GGGAGGACCTGCAGAGCACAGCCCCGATCCCATCCGTCCCCTACGCGCCCTTTGCTGCTA
  TTCTGCCCTTTCAGCATGGAGGTTCCTCAGCCCCTGTCGAATTTGTGGGTGATTTTACTG
  CTCCTGAGTCAACCGGTGACCCAGAAAAACCACCTCCTCTACCAGAGAAGAAAAACAAAC
  ACATGCTGGCCTACATGCAGTTGCTGGAGGACTACTCGGAGCCGCAGCCCTCTATGTTCT
  ACCAGACGCCACAGAACGAGCACATCTACCAGCAGAAGAACAAGCTCCTCATGGAGGTAT
  ACGGCTTCAGCGACTCCTTCAGTGGGGTGGACTCCGTGCAGGAGCTGGCCCCGCCGCCCG
  CCCTACCCCCCAAGCAGCGGCAGCTGGAGCCACCGGCTGGGAAAGACGGACATCCCAGAG
  ATCCCTCAGCGGTCAGCGGCGTCCCTGGGAAGGACAGCAGAGACGGCAGTGAGAGGGCCC
  CAAAGTCACCAGATGCTCTGGAGTCGGCTCAGTCGGAGGAGGAAGTGGACGAGCTGTCCC
  TCATTGACCACAACGAAATTATGTCCAGGCTGACGCTCAAGCAGGAGGGTGATGACGGGC
  CGGACGTCCGCGGAGGATCTGGGGACATCTTACTGGTCCATGCTACTGAGACTGACAGGA
  AAGATTTGGTGTTGTACTGCGAGGCATTCCTGACCACCTACAGGACCTTCATCTCCCCAG
  AGGAGCTCATCAAGAAGCTGCAGTACAGATATGAGAAATTCTCTCCCTTTGCCGACACAT
  TCAAGAAGCGCGTCAGCAAGAACACGTTCTTCGTGCTGGTACGGGTGGTGGATGAGCTCT
  GCCTGGTGGAGTTGACAGAAGAGATCCTGAAGCTGCTGATGGAACTGGTCTTCCGCCTGG
  TGTGCAATGGGGAGCTGAGCCTGGCCCGTGTGCTCCGGAAGAACATCCTGGACAAGGTGG
  ACCAGAAGAAGCTACTCAGGTGTGCCACCTCCAGCCAGCCCCTGGCAGCCCGGGGGGTAG
  CAGCCAGGCCGGGGACCTTGCACGACTTTCACAGCCATGAGATAGCGGAGCAGCTAACGC
  TGCTGGATGCTGAGCTCTTCTATAAAATAGAGATTCCTGAGGTTTTGCTTTGGGCAAAAG
  AGCAGAATGAGGAGAAGAGCCCCAACTTGACCCAGTTCACGGAGCACTTCAACAACATGT
  CCTACTGGGTCCGGTCCATAATCATGTTACAGGAAAAGGCCCAGGACAGGGAACGGCTGC
  TCTTGAAGTTCATCAAGATCATGAAGCACTTGCGGAAGCTGAATAACTTCAACTCCTACT
  TGGCCATCCTCTCTGCCCTGGACTCGGCGCCCATCCGCAGGCTGGAGTGGCAGAAGCAGA
  CTTCAGAGGGCCTGGCCGAGTACTGCACACTGATCGACAGCTCGTCCTCCTTCCGAGCCT
  ACCGGGCCGCCCTCTCGGAGGTGGAACCGCCGTGCATCCCGTACCTGGGGCTGATCCTGC
  AGGACCTGACCTTCGTTCACCTGGGAAACCCAGACTACATCGACGGGAAAGTGAACTTCT
  CCAAGCGGTGGCAGCAGTTCAACATCCTCGACAGCATGCGCTGCTTCCAGCAGGCGCACT
  ATGACATGCGGAGGAACGACGACATTATAAACTTCTTCAATGACTTCAGTGACCACCTGG
  CTGAGGAGGCCCTATGGGAACTGTCTCTGAAAATTAAACCCAGGAACATAACAAGGAGAA
  AAACAGACCGGGAAGAGAAGACCTAGGAGCAGACGCCGGGATCCAGGAGAATGCTCGAGG
  GGCGCAGAGGGCAGCTCCCAGACCGGAGAGGACCTTGGACCTGTTAGGCGCATGGCAGGA
  GTCCCGGCCTCGGAGCCATGAGGCTGGCCAGCCCTCAGCGGGGCCGGGCGGGAGCTGGAG
  CCTGCCAGCCGCTTCCTGCCTCCTTCCTCTGTGGGAGCAGACCCGTGGGCCTCAGGGCAG
  CCAGCAGGCAGGTCTTGTTGCCAATTTACAAACCGGTGGTTTTCTGGTTTGGTTTTGTTT
  TCTGCTTTTACTTCCATCTCTCCCCTCTTGACCTTCCACCCACTCCCCTCCAGGGAGAGA
  GCAGCAGAGACCTCATCAGCAGACCAAGGAAGTGGTGGGTGCTCCCCCTCCCTAAGCTCC
  AGGGTCCCTGAATCTTCTGAAATCTCAAATGAGTGGAGGCCTCCTGGGGTGGCCTGTCCT
  GCAGGGGCCCTGGAATGGGGGCAAGCAGCTGGGTGGGCAGAATGCAGAGTAGACTCGGGG
  GAGGATCCTTTCACTTTCCGCTTCCCCTTCTGATGCATGGAGGATGGTGTGAGCTTTTCA
  GCAGGCCCGGAAAGGTACGCAGGTGACGCCTTAGCAGCCCCGCAGCTGGTGCTCTGCCCC
  GCGGTACTGGCGCCATCAGGGCCTCCCTTGCCCGCCTGAGAGCAGCAGCAGTCTCTGTCA
  TCCCGTCGCCCCTTACCCCCCACCCCAGGCCACTGGGCCCCTCCCACACCACCTGGGGAG
  CTGAGAAGAGGAGGCTGGAGTAAGGGAGGACTTGATCATCCAAGAAATACTTTTTATTGC
  TGGGAGTCTTCTGAACCTCACCAAACTGAGGCCAGAGCTGAGCTCCTGGGGGAGTTAATT
  CAGAGGGGAGAGGCCAGCACCTCCCTCCTCCATTGCTCGCTGTGTGCCTTAAACTCCATC
  TCATGTCCCTCCCCATCCCCTGGCTTTCCCTCCCTCCTTGCCCCATCCTGGGCCAGCCAG
  CAGGGCTCCTCCTCTGGCTCTTCAGACCTTTCAGCCAGTGCTGTCAGTGCCCCTGGGAGG
  GAAGGGCATCCCTGAGGCACCCGAATGGTCCCTCAGGGTGCAGGGAGGCAGAAGCCTGGC
  CACAGAGGAGCCTCCTAAGGCAGCAGCTGCAGCAAGCGCACCCTCTCCCCACTCTCCCCA
  CGCCAGAGCGGCTTCCAGAGCAGATGCTGTTTCCATCCTCCTCGTCAAAACCATTCTCGC
  TGCTGAGCTTGACAATCTGGGCAAGGCTTGTGGGGCGCTTGACAAACAGAATCTGCCCTG
  TGCCGCCTGGTTCCGTGGCCTCCAGCATGAGCCTGCAGGCAGGGCGCTGCGGGAACCCAG
  TTGTGCTGCCCCAGCCCATGCCTCCGGGTCTGCTGTGCATGAATGAGTGCTCACTTGTCC
  CGGGTTTAGGACGTGGTCAAGTGAACAGCAGGGTCTAACTGTGCTTACTTAGCCCAGTTC
  AAACAGAACAAAGGAAAAATATAGAAAGCAACATCTGTTGATCATTTAGGTTTTTTTTTA
  AACCACCATGTCACTTTGAGTCCTTCATGGGTTTTTGAACAGCATTTATCAAGAAGAAAA
  TGTGGGCTTTTTCCCCTCTCCCGTGTTTTGTTTGTCCTGTAGATAGAGGGAGGAAAGCCG
  TGCAGTGGCAGGCGGGACCCCCTCTGGTGGCGGGACCCCCTCTTGCGGTGGTCTTGCGGG
  GCCAGCCGGGACCTGTCACTTTATTATTTAAGGAGTGTGTGTGTAGAGTCGCTGGCTTAT
  TAACAGTATTGTGTGTGGGTTGGGTTTTTAGTTTGTTCCTTCTTTTTGAAGTCCCTTCAT
  TTCAATCCTTGACTCTCTCTCCCCTTCCCTTGCCCAGCTCTGTTGAATGCTGCTGTGCGC
  GTGTGAGGGCCGCTCTGCACACAGGGCCCTTGGGTTGTGTGAACTGAAATTCTCCCTGTA
  TTTGTGAGACTCGCAGGAGTCCCCATCTGTAGCACAGGCAATGCCAGTGCCATGCTGCAG
  CCTCAGAAACCAGGCCTCTCACTCCAGCAGCAGGCAGAACCGTGTCTGTGGTCGGGTGCT
  GTCCACAGCTCTGTCTGCCTTGTTCTTGGGCTTGAGCTGGATAGAGGTGGGGTCTCTTCA
  CCTTCCCTGAATTCAGAACAGACCCTGTGCCTGGCCCCAGTGTGCCCAGGCAATTCCCCA
  GGCCCTCATTGGGAGCCCTTGGTGTTCTGAGCAGCAGGGCCCAGGCAGCACATGAGCAGT
  GCCCAGGGGCTCCCTGCGTGAGGACGGCAAGGTGCGATGTATGTCTAACTTATTGATGGC
  AGGCAGCCCCCTGTGCCCCCTAAGCCTGGCCCTGGTTATTGCTGAGCTCTGTGCTCAGTG
  CTGCGGCCTGGCCGTGGCTCGTCTGTTCCTTTGGGGGGCCCGGGCGGGTTGTGGGAATCA
  GTCTTCACAGACAGACGTGAGCCAGGCGGAGGACTCGTTCCTTGCAGAGGTCAGTCCTCA
  CCTGCAGGTGTCGGGGTGGGGGGGGGCAAGGAGGGGCAGGCACACACCATGTCTGACCTG
  AACCCGATTCTGGGGAGCATCTTCCCGCTCCGGCCCCACGACCTCCACAGGGTTACATTG
  TAATATATATGCCCCAGCTAACCTGTCTGATGGTGGCATCTTCCTGCAGACATTTCAAAC
  ATGTAACTTTTATATGAAAAAAAATAAACACAGATGAAAGCTGCCAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_198679
• Insert Size: 6100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198679.1, NP_941372.1
• RefSeq Size: 6204 bp
• RefSeq ORF: 3288 bp
• Locus ID: 2889
• Cytogenetics: 9q34.13
• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, Insulin signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma
• Gene Summary: 'This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) encodes the longest isoform (b).