GRF2 (RAPGEF1) (NM_198679) Human Untagged Clone

CAT#: SC307763

RAPGEF1 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2


  "NM_198679" in other vectors (4)

Reconstitution Protocol

USD 1,100.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol RAPGEF1
Synonyms C3G; GRF2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_198679 edited
CTGGGGCTGGGAAGGGCCCTGTGTCCTCTGGCTTTAAAATGGGCAATGCTATTGAAAAAC
AGAAGCCCTTGAAACGAAGTCATCTGTACCCCTGGAAACAAGACTCTCAGCGTTCTCATC
TCTCTTCCTTCACCATGAAGCTGATGGACAAATTCCACTCACCCAAAATCAAGAGAACGC
CATCAAAGAAGGGAAAACCAGCTGAGGTGTCCGTAAAGATTCCAGAGAAGCCTGTGAACA
AAGAGGCAACAGACAGATTTCTACCAGAGGGCTACCCTCTCCCCTTGGATCTGGAGCAGC
AGGCAGTAGAATTTATGTCCACCAGTGCTGTGGCTTCCAGGTCTCAAAGGCAGAAGAACC
TGAGCTGGCTGGAGGAGAAAGAGAAGGAAGTTGTCAGTGCCCTGCGCTACTTTAAGACCA
TTGTGGACAAAATGGCAATTGATAAGAAGGTACTGGAGATGCTTCCAGGGTCAGCCAGCA
AGGTGCTGGAGGCCATCTTACCCCTGGTGCAGAACGATCCTCGAATTCAGCACAGCTCAG
CCCTCTCTTCCTGCTATAGCCGAGTGTACCAAAGCCTCGCCAACCTCATTCGCTGGTCTG
ACCAAGTGATGCTGGAAGGCGTGAACTCAGAAGACAAGGAGATGGTGACGACTGTGAAGG
GGGTCATCAAGGCTGTGCTGGATGGAGTGAAGGAGCTGGTCAGGCTCACCATCGAGAAGC
AGGGACGTCCGTCTCCGACGAGCCCCGTGAAGCCCAGTTCCCCTGCCAGCAAGCCTGATG
GCCCAGCAGAGCTCCCCCTGACAGACCGCGAGGTAGAGATCCTAAACAAGACGACTGGGA
TGTCACAGTCAACTGAGCTCCTCCCAGATGCCACGGATGAAGAGGTCGCGCCCCCCAAGC
CTCCTCTGCCTGGCATTCGGGTGGTTGATAATAGTCCTCCACCAGCATTGCCACCCAAGA
AAAGACAGTCGGCGCCGTCCCCTACCCGAGTGGCTGTGGTGGCCCCCATGAGCCGAGCCA
CCAGTGGCTCCAGTTTGCCTGTTGGAATCAATAGGCAGGATTTTGATGTTGACTGTTACG
CACAGAGGCGACTGTCAGGAGGCAGCCACTCATATGGTGGAGAGTCGCCCCGCCTCTCCC
CCTGCAGCAGCATAGGCAAGCTCAGCAAGTCAGACGAGCAGCTGTCCTCTCTGGACAGGG
ACAGTGGGCAGTGCTCCCGGAACACAAGCTGTGAAACACTAGACCACTATGATCCCGACT
ATGAATTCCTCCAGCAAGACCTCTCTAACGCAGACCAGATACCTCAGCAGACGGCCTGGA
ACCTTAGCCCGTTGCCAGAGTCTTTGGGGGAGTCTGGGTCTCCATTTCTTGGCCCTCCTT
TCCAGCTGCCTCTTGGCGGCCATCCCCAGCCAGACGGACCTCTGGCCCCAGGGCAGCAGA
CAGATACGCCACCTGCTCTCCCCGAGAAGAAGCGCAGGAGCGCAGCCTCCCAGACGGCGG
ACGGCTCTGGCTGCAGGGTGTCCTACGAGCGGCATCCCTCGCAGTATGACAACATCTCTG
GGGAGGACCTGCAGAGCACAGCCCCGATCCCATCCGTCCCCTACGCGCCCTTTGCTGCTA
TTCTGCCCTTTCAGCATGGAGGTTCCTCAGCCCCTGTCGAATTTGTGGGTGATTTTACTG
CTCCTGAGTCAACCGGTGACCCAGAAAAACCACCTCCTCTACCAGAGAAGAAAAACAAAC
ACATGCTGGCCTACATGCAGTTGCTGGAGGACTACTCGGAGCCGCAGCCCTCTATGTTCT
ACCAGACGCCACAGAACGAGCACATCTACCAGCAGAAGAACAAGCTCCTCATGGAGGTAT
ACGGCTTCAGCGACTCCTTCAGTGGGGTGGACTCCGTGCAGGAGCTGGCCCCGCCGCCCG
CCCTACCCCCCAAGCAGCGGCAGCTGGAGCCACCGGCTGGGAAAGACGGACATCCCAGAG
ATCCCTCAGCGGTCAGCGGCGTCCCTGGGAAGGACAGCAGAGACGGCAGTGAGAGGGCCC
CAAAGTCACCAGATGCTCTGGAGTCGGCTCAGTCGGAGGAGGAAGTGGACGAGCTGTCCC
TCATTGACCACAACGAAATTATGTCCAGGCTGACGCTCAAGCAGGAGGGTGATGACGGGC
CGGACGTCCGCGGAGGATCTGGGGACATCTTACTGGTCCATGCTACTGAGACTGACAGGA
AAGATTTGGTGTTGTACTGCGAGGCATTCCTGACCACCTACAGGACCTTCATCTCCCCAG
AGGAGCTCATCAAGAAGCTGCAGTACAGATATGAGAAATTCTCTCCCTTTGCCGACACAT
TCAAGAAGCGCGTCAGCAAGAACACGTTCTTCGTGCTGGTACGGGTGGTGGATGAGCTCT
GCCTGGTGGAGTTGACAGAAGAGATCCTGAAGCTGCTGATGGAACTGGTCTTCCGCCTGG
TGTGCAATGGGGAGCTGAGCCTGGCCCGTGTGCTCCGGAAGAACATCCTGGACAAGGTGG
ACCAGAAGAAGCTACTCAGGTGTGCCACCTCCAGCCAGCCCCTGGCAGCCCGGGGGGTAG
CAGCCAGGCCGGGGACCTTGCACGACTTTCACAGCCATGAGATAGCGGAGCAGCTAACGC
TGCTGGATGCTGAGCTCTTCTATAAAATAGAGATTCCTGAGGTTTTGCTTTGGGCAAAAG
AGCAGAATGAGGAGAAGAGCCCCAACTTGACCCAGTTCACGGAGCACTTCAACAACATGT
CCTACTGGGTCCGGTCCATAATCATGTTACAGGAAAAGGCCCAGGACAGGGAACGGCTGC
TCTTGAAGTTCATCAAGATCATGAAGCACTTGCGGAAGCTGAATAACTTCAACTCCTACT
TGGCCATCCTCTCTGCCCTGGACTCGGCGCCCATCCGCAGGCTGGAGTGGCAGAAGCAGA
CTTCAGAGGGCCTGGCCGAGTACTGCACACTGATCGACAGCTCGTCCTCCTTCCGAGCCT
ACCGGGCCGCCCTCTCGGAGGTGGAACCGCCGTGCATCCCGTACCTGGGGCTGATCCTGC
AGGACCTGACCTTCGTTCACCTGGGAAACCCAGACTACATCGACGGGAAAGTGAACTTCT
CCAAGCGGTGGCAGCAGTTCAACATCCTCGACAGCATGCGCTGCTTCCAGCAGGCGCACT
ATGACATGCGGAGGAACGACGACATTATAAACTTCTTCAATGACTTCAGTGACCACCTGG
CTGAGGAGGCCCTATGGGAACTGTCTCTGAAAATTAAACCCAGGAACATAACAAGGAGAA
AAACAGACCGGGAAGAGAAGACCTAGGAGCAGACGCCGGGATCCAGGAGAATGCTCGAGG
GGCGCAGAGGGCAGCTCCCAGACCGGAGAGGACCTTGGACCTGTTAGGCGCATGGCAGGA
GTCCCGGCCTCGGAGCCATGAGGCTGGCCAGCCCTCAGCGGGGCCGGGCGGGAGCTGGAG
CCTGCCAGCCGCTTCCTGCCTCCTTCCTCTGTGGGAGCAGACCCGTGGGCCTCAGGGCAG
CCAGCAGGCAGGTCTTGTTGCCAATTTACAAACCGGTGGTTTTCTGGTTTGGTTTTGTTT
TCTGCTTTTACTTCCATCTCTCCCCTCTTGACCTTCCACCCACTCCCCTCCAGGGAGAGA
GCAGCAGAGACCTCATCAGCAGACCAAGGAAGTGGTGGGTGCTCCCCCTCCCTAAGCTCC
AGGGTCCCTGAATCTTCTGAAATCTCAAATGAGTGGAGGCCTCCTGGGGTGGCCTGTCCT
GCAGGGGCCCTGGAATGGGGGCAAGCAGCTGGGTGGGCAGAATGCAGAGTAGACTCGGGG
GAGGATCCTTTCACTTTCCGCTTCCCCTTCTGATGCATGGAGGATGGTGTGAGCTTTTCA
GCAGGCCCGGAAAGGTACGCAGGTGACGCCTTAGCAGCCCCGCAGCTGGTGCTCTGCCCC
GCGGTACTGGCGCCATCAGGGCCTCCCTTGCCCGCCTGAGAGCAGCAGCAGTCTCTGTCA
TCCCGTCGCCCCTTACCCCCCACCCCAGGCCACTGGGCCCCTCCCACACCACCTGGGGAG
CTGAGAAGAGGAGGCTGGAGTAAGGGAGGACTTGATCATCCAAGAAATACTTTTTATTGC
TGGGAGTCTTCTGAACCTCACCAAACTGAGGCCAGAGCTGAGCTCCTGGGGGAGTTAATT
CAGAGGGGAGAGGCCAGCACCTCCCTCCTCCATTGCTCGCTGTGTGCCTTAAACTCCATC
TCATGTCCCTCCCCATCCCCTGGCTTTCCCTCCCTCCTTGCCCCATCCTGGGCCAGCCAG
CAGGGCTCCTCCTCTGGCTCTTCAGACCTTTCAGCCAGTGCTGTCAGTGCCCCTGGGAGG
GAAGGGCATCCCTGAGGCACCCGAATGGTCCCTCAGGGTGCAGGGAGGCAGAAGCCTGGC
CACAGAGGAGCCTCCTAAGGCAGCAGCTGCAGCAAGCGCACCCTCTCCCCACTCTCCCCA
CGCCAGAGCGGCTTCCAGAGCAGATGCTGTTTCCATCCTCCTCGTCAAAACCATTCTCGC
TGCTGAGCTTGACAATCTGGGCAAGGCTTGTGGGGCGCTTGACAAACAGAATCTGCCCTG
TGCCGCCTGGTTCCGTGGCCTCCAGCATGAGCCTGCAGGCAGGGCGCTGCGGGAACCCAG
TTGTGCTGCCCCAGCCCATGCCTCCGGGTCTGCTGTGCATGAATGAGTGCTCACTTGTCC
CGGGTTTAGGACGTGGTCAAGTGAACAGCAGGGTCTAACTGTGCTTACTTAGCCCAGTTC
AAACAGAACAAAGGAAAAATATAGAAAGCAACATCTGTTGATCATTTAGGTTTTTTTTTA
AACCACCATGTCACTTTGAGTCCTTCATGGGTTTTTGAACAGCATTTATCAAGAAGAAAA
TGTGGGCTTTTTCCCCTCTCCCGTGTTTTGTTTGTCCTGTAGATAGAGGGAGGAAAGCCG
TGCAGTGGCAGGCGGGACCCCCTCTGGTGGCGGGACCCCCTCTTGCGGTGGTCTTGCGGG
GCCAGCCGGGACCTGTCACTTTATTATTTAAGGAGTGTGTGTGTAGAGTCGCTGGCTTAT
TAACAGTATTGTGTGTGGGTTGGGTTTTTAGTTTGTTCCTTCTTTTTGAAGTCCCTTCAT
TTCAATCCTTGACTCTCTCTCCCCTTCCCTTGCCCAGCTCTGTTGAATGCTGCTGTGCGC
GTGTGAGGGCCGCTCTGCACACAGGGCCCTTGGGTTGTGTGAACTGAAATTCTCCCTGTA
TTTGTGAGACTCGCAGGAGTCCCCATCTGTAGCACAGGCAATGCCAGTGCCATGCTGCAG
CCTCAGAAACCAGGCCTCTCACTCCAGCAGCAGGCAGAACCGTGTCTGTGGTCGGGTGCT
GTCCACAGCTCTGTCTGCCTTGTTCTTGGGCTTGAGCTGGATAGAGGTGGGGTCTCTTCA
CCTTCCCTGAATTCAGAACAGACCCTGTGCCTGGCCCCAGTGTGCCCAGGCAATTCCCCA
GGCCCTCATTGGGAGCCCTTGGTGTTCTGAGCAGCAGGGCCCAGGCAGCACATGAGCAGT
GCCCAGGGGCTCCCTGCGTGAGGACGGCAAGGTGCGATGTATGTCTAACTTATTGATGGC
AGGCAGCCCCCTGTGCCCCCTAAGCCTGGCCCTGGTTATTGCTGAGCTCTGTGCTCAGTG
CTGCGGCCTGGCCGTGGCTCGTCTGTTCCTTTGGGGGGCCCGGGCGGGTTGTGGGAATCA
GTCTTCACAGACAGACGTGAGCCAGGCGGAGGACTCGTTCCTTGCAGAGGTCAGTCCTCA
CCTGCAGGTGTCGGGGTGGGGGGGGGCAAGGAGGGGCAGGCACACACCATGTCTGACCTG
AACCCGATTCTGGGGAGCATCTTCCCGCTCCGGCCCCACGACCTCCACAGGGTTACATTG
TAATATATATGCCCCAGCTAACCTGTCTGATGGTGGCATCTTCCTGCAGACATTTCAAAC
ATGTAACTTTTATATGAAAAAAAATAAACACAGATGAAAGCTGCCAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_198679
Insert Size 6100 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_198679.1, NP_941372.1
RefSeq Size 6204 bp
RefSeq ORF 3288 bp
Locus ID 2889
Cytogenetics 9q34.13
Protein Families Druggable Genome
Protein Pathways Focal adhesion, Insulin signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma
Gene Summary 'This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2) encodes the longest isoform (b).

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