APOL3 (NM_014349) Human Tagged ORF Clone

CAT#: RG208325

APOL3 (GFP-tagged) - Human apolipoprotein L, 3 (APOL3), transcript variant alpha/a

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: APOL3
• Synonyms: apoL-III; APOLIII; CG12_1; CG121
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208325 representing NM_014349
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCAGAAAAGAAACGCTTTACTGAAGAGGCCACCAAATACTTCCGGGAGAGAGTCAGCCCAGTGC
  ATCTGCAAATCCTGCTGACTAACAATGAAGCCTGGAAGAGATTCGTGACTGCGGCTGAATTGCCCAGGGA
  TGAGGCAGATGCTCTCTACGAAGCTCTGAAGAAGCTTAGAACATATGCAGCTATTGAGGACGAATATGTG
  CAGCAGAAAGATGAGCAGTTTAGGGAATGGTTTTTGAAAGAGTTTCCCCAAGTCAAGAGGAAGATCCAGG
  AGTCCATAGAAAAGCTTCGTGCCCTTGCAAATGGTATTGAAGAGGTCCACAGAGGCTGCACCATCTCCAA
  TGTGGTGTCCAGCTCCACTGGCGCTGCCTCTGGCATCATGTCCCTTGCTGGTCTTGTTTTGGCACCATTT
  ACAGCAGGGACGAGTCTGGCCCTTACTGCAGCTGGGGTAGGGCTGGGAGCAGCGTCTGCTGTGACTGGGA
  TCACCACCAGCATCGTGGAGCACTCATACACATCATCAGCAGAAGCTGAAGCCAGCAGGCTGACTGCAAC
  CAGCATTGACCGATTGAAGGTATTTAAGGAAGTTATGCGTGACATCACACCCAACTTACTTTCCCTTCTT
  AATAATTATTACGAAGCCACACAAACCATTGGGAGTGAAATCCGTGCCATCAGGCAAGCCAGAGCCAGGG
  CCCGACTCCCTGTGACCACCTGGCGAATCTCAGCTGGAAGTGGAGGTCAAGCAGAGAGAACGATTGCAGG
  CACCACCCGGGCAGTGAGCAGAGGAGCCCGGATCCTGAGTGCGACCACTTCAGGCATCTTCCTTGCACTG
  GATGTGGTCAACCTTGTATACGAGTCAAAGCACTTGCATGAGGGGGCAAAGTCTGCATCTGCTGAGGAGC
  TGAGGCGGCAGGCTCAGGAGCTGGAGGAGAATCTAATGGAGCTCACTCAGATCTATCAGCGTCTGAATCC
  ATGCCATACCCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208325 representing NM_014349
  Red=Cloning site Green=Tags(s)
  
  MDSEKKRFTEEATKYFRERVSPVHLQILLTNNEAWKRFVTAAELPRDEADALYEALKKLRTYAAIEDEYV
  QQKDEQFREWFLKEFPQVKRKIQESIEKLRALANGIEEVHRGCTISNVVSSSTGAASGIMSLAGLVLAPF
  TAGTSLALTAAGVGLGAASAVTGITTSIVEHSYTSSAEAEASRLTATSIDRLKVFKEVMRDITPNLLSLL
  NNYYEATQTIGSEIRAIRQARARARLPVTTWRISAGSGGQAERTIAGTTRAVSRGARILSATTSGIFLAL
  DVVNLVYESKHLHEGAKSASAEELRRQAQELEENLMELTQIYQRLNPCHTH
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014349
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_014349.2, NP_055164.1
• RefSeq Size: 2444
• RefSeq ORF: 996
• Locus ID: 80833
• Gene Summary: This gene is a member of the apolipoprotein L gene family, and it is present in a cluster with other family members on chromosome 22. The encoded protein is found in the cytoplasm, where it may affect the movement of lipids, including cholesterol, and/or allow the binding of lipids to organelles. In addition, expression of this gene is up-regulated by tumor necrosis factor-alpha in endothelial cells lining the normal and atherosclerotic iliac artery and aorta. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]