EYA4 (NM_172103) Human Tagged ORF Clone

CAT#: RG208336

EYA4 (GFP-tagged) - Human eyes absent homolog 4 (Drosophila) (EYA4), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EYA4
• Synonyms: CMD1J; DFNA10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208336 representing NM_172103
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACTCCCAGGATTTAAATGAACAATCAGTAAAGAAAACGTGCACAGAATCAGATGTTTCACAAT
  CTCAGAATTCCAGGTCTATGGAAATGCAGGACCTAGCAAGTCCTCATACTCTTGTTGGAGGTGGTGATAC
  TCCAGGTAGCTCCAAACTGGAAAAATCTAATCTCAGCAGCACATCAGTTACTACAAATGGGACAGGAGTG
  TCTCTTCTTGCAGTCAAAACAGAGCCCTTGAACAGCAGTGAAACCACAGCCACGACTGGAGATGGAGCGC
  TTGACACTTTTACTGGGTCAGTAATTACAAGTAGTGGCTACAGCCCCAGATCAGCACATCAGTATTCCCC
  ACAGCTGTATCCTTCCAAGCCCTATCCACACATTCTTTCTACACCAGCAGCTCAAACAATGTCTGCCTAT
  GCAGGCCAGACTCAGTATTCGGGGATGCAGCAGCCAGCCGTCTACACAGCCTACTCACAGACAGGACAGC
  CCTACAGCTTGCCCACTTACGATTTGGGTGTGATGTTGCCAGCCATCAAGACAGAGAGTGGACTTTCCCA
  AACTCAGTCCCCATTACAGAGTGGCTGCCTCAGTTACAGCCCAGGGTTCTCTACCCCACAGCCAGGCCAG
  ACACCTTATTCTTACCAAATGCCAGGTTCTAGTTTTGCACCATCATCTACTATTTATGCAAATAATTCAG
  TTTCCAATTCAACAAATTTCAGTGGTTCACAACAGGATTATCCATCCTATACAGCCTTTGGCCAAAACCA
  GTATGCACAGTATTATTCAGCATCAACGTATGGAGCGTATATGACATCGAATAACACAGCCGATGGCACA
  CCCTCTTCAACCTCTACTTATCAGTTGCAGGAATCTCTCCCAGGACTGACTAACCAACCAGGAGAGTTCG
  ATACCATGCAGAGTCCCTCCACACCCATCAAAGATCTTGATGAGAGAACCTGTAGGAGTTCTGGGTCAAA
  GTCCAGAGGAAGAGGCCGGAAAAATAATCCCTCCCCGCCTCCTGATAGTGACCTGGAGCGTGTGTTTGTC
  TGGGATTTGGATGAAACCATCATTGTTTTTCACTCACTGCTCACCGGGTCTTATGCACAGAAGTATGGCA
  AGGATCCCCCCATGGCTGTAACCCTTGGACTCCGCATGGAAGAAATGATTTTTAATCTTGCTGATACTCA
  TTTGTTTTTTAATGATTTAGAGGAGTGTGATCAAGTTCATATAGATGATGTTTCCTCTGATGATAATGGG
  CAGGACTTAAGTACCTACAGTTTTGCAACTGATGGCTTCCATGCAGCTGCAAGTAGTGCAAACCTTTGTT
  TGCCAACAGGTGTAAGAGGAGGGGTTGACTGGATGAGGAAGTTGGCTTTTCGTTACAGAAGAGTAAAAGA
  ATTATATAACACCTACAAGAACAACGTTGGAGGACTCCTTGGCCCTGCCAAGAGGGATGCCTGGCTACAG
  TTAAGGGCAGAGATTGAAGGTCTGACAGATTCCTGGCTAACAAATGCACTTAAGTCTTTATCAATTATTA
  GCACTAGGAGTAACTGCATAAATGTCTTGGTAACGACAACTCAACTGATCCCAGCACTTGCGAAGGTTCT
  ACTCTATAGTTTAGGAGGTGCTTTCCCCATTGAGAATATTTACAGTGCAACTAAAATAGGAAAAGAAAGT
  TGCTTTGAACGAATAATGCAAAGGTTTGGCAGAAAAGTAGTGTATGTTGTAATTGGGGATGGTGTAGAAG
  AAGAACAGGCAGCAAAAAAGCACAACATGCCCTTCTGGAGGATATCCAGTCACTCAGACCTCCTGGCTCT
  CCACCAAGCACTGGAATTAGAGTATTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208336 representing NM_172103
  Red=Cloning site Green=Tags(s)
  
  MEDSQDLNEQSVKKTCTESDVSQSQNSRSMEMQDLASPHTLVGGGDTPGSSKLEKSNLSSTSVTTNGTGV
  SLLAVKTEPLNSSETTATTGDGALDTFTGSVITSSGYSPRSAHQYSPQLYPSKPYPHILSTPAAQTMSAY
  AGQTQYSGMQQPAVYTAYSQTGQPYSLPTYDLGVMLPAIKTESGLSQTQSPLQSGCLSYSPGFSTPQPGQ
  TPYSYQMPGSSFAPSSTIYANNSVSNSTNFSGSQQDYPSYTAFGQNQYAQYYSASTYGAYMTSNNTADGT
  PSSTSTYQLQESLPGLTNQPGEFDTMQSPSTPIKDLDERTCRSSGSKSRGRGRKNNPSPPPDSDLERVFV
  WDLDETIIVFHSLLTGSYAQKYGKDPPMAVTLGLRMEEMIFNLADTHLFFNDLEECDQVHIDDVSSDDNG
  QDLSTYSFATDGFHAAASSANLCLPTGVRGGVDWMRKLAFRYRRVKELYNTYKNNVGGLLGPAKRDAWLQ
  LRAEIEGLTDSWLTNALKSLSIISTRSNCINVLVTTTQLIPALAKVLLYSLGGAFPIENIYSATKIGKES
  CFERIMQRFGRKVVYVVIGDGVEEEQAAKKHNMPFWRISSHSDLLALHQALELEYL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172103
• ORF Size: 1848 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_172103.2, NP_742101.2
• RefSeq Size: 5031
• RefSeq ORF: 1851
• Locus ID: 2070
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Gene Summary: This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator through its protein phosphatase activity, and it may be important for eye development, and for continued function of the mature organ of Corti. Mutations in this gene are associated with postlingual, progressive, autosomal dominant hearing loss at the deafness, autosomal dominant non-syndromic sensorineural 10 locus. The encoded protein is also a putative oncogene that mediates DNA repair, apoptosis, and innate immunity following DNA damage, cellular damage, and viral attack. Defects in this gene are also associated with dilated cardiomyopathy 1J. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]