IRAKM (IRAK3) (NM_007199) Human Tagged ORF Clone

CAT#: RG209245

IRAK3 (GFP-tagged) - Human interleukin-1 receptor-associated kinase 3 (IRAK3), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: IRAK3
• Synonyms: ASRT5; IRAKM
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209245 representing NM_007199
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGAACTGTGGGGCCCGCGGCGCGCTGTCGGCGCACACGCTGCTGTTCGACCTGCCGCCCGCGC
  TGCTCGGAGAGCTCTGCGCTGTTCTGGACAGCTGCGACGGCGCGCTGGGCTGGCGCGGCCTGGCAGAGAG
  ACTTTCAAGCAGCTGGCTGGATGTTCGTCATATTGAAAAGTATGTAGACCAAGGTAAAAGTGGAACAAGA
  GAATTACTTTGGTCCTGGGCACAGAAAAACAAGACCATCGGTGACCTTTTACAGGTCCTCCAGGAGATGG
  GACATCGTCGAGCTATTCATTTAATTACAAACTATGGAGCAGTGTTGAGTCCTTCAGAGAAGAGTTATCA
  GGAAGGTGGATTTCCAAATATATTATTCAAGGAAACAGCCAATGTCACCGTGGATAATGTTCTTATTCCT
  GAACATAATGAAAAAGGAGTACTGCTTAAATCTTCCATCAGCTTTCAAAATATCATAGAAGGAACTAGAA
  ATTTCCACAAAGACTTCCTAATTGGAGAAGGAGAGATTTTTGAGGTATACAGAGTGGAGATTCAAAACCT
  AACATATGCTGTCAAATTATTTAAACAGGAGAAAAAAATGCAGTGTAAGAAGCATTGGAAGAGGTTTTTA
  TCTGAGCTTGAAGTTTTACTACTGTTTCATCACCCAAACATACTAGAGTTGGCTGCATATTTTACAGAGA
  CTGAGAAGTTCTGTCTGATTTATCCATACATGAGAAATGGAACACTTTTTGACAGATTGCAGTGTGTAGG
  TGACACGGCCCCACTCCCTTGGCACATTCGAATCGGTATATTAATAGGAATATCCAAAGCCATTCACTAC
  CTGCACAACGTTCAACCATGCTCGGTCATCTGTGGCAGTATATCAAGTGCAAACATCCTTTTGGATGATC
  AGTTTCAACCCAAACTAACTGATTTTGCCATGGCACACTTCCGGTCCCACCTAGAACATCAGAGTTGTAC
  CATAAATATGACCAGCAGCAGCAGTAAACATCTGTGGTACATGCCAGAAGAGTACATCAGACAGGGGAAA
  CTTTCCATTAAAACAGATGTCTACAGCTTTGGAATTGTAATAATGGAAGTTCTAACAGGATGTAGAGTAG
  TGTTAGATGATCCAAAACATATCCAGCTGCGGGATCTCCTTAGAGAATTGATGGAGAAGAGAGGCCTGGA
  TTCATGTCTCTCATTTCTAGATAAGAAAGTGCCTCCCTGCCCTCGGAATTTCTCTGCCAAGCTCTTCTGT
  TTGGCAGGCCGGTGTGCTGCAACGCGGGCAAAGTTAAGACCATCAATGGATGAAGTTTTAAATACTCTTG
  AAAGTACTCAAGCCAGCTTGTATTTTGCTGAAGATCCTCCCACATCACTAAAGTCCTTCAGGTGTCCTTC
  TCCTCTATTCCTGGAGAATGTACCAAGTATTCCAGTGGAAGATGATGAAAGCCAGAATAACAATTTACTA
  CCTTCTGATGAAGGCCTGAGGATAGACAGAATGACTCAGAAAACTCCTTTTGAATGCAGCCAGTCTGAGG
  TTATGTTTCTGAGCTTGGACAAAAAGCCAGAGAGCAAGAGAAATGAGGAAGCTTGCAACATGCCCAGTTC
  TTCTTGTGAAGAAAGTTGGTTCCCAAAGTATATAGTTCCATCCCAGGACTTAAGGCCCTATAAGGTAAAT
  ATAGATCCTTCTTCAGAAGCTCCAGGGCATTCTTGCAGGAGCAGGCCAGTGGAGAGCAGCTGTTCCTCCA
  AATTTTCCTGGGATGAATATGAACAGTACAAAAAAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209245 representing NM_007199
  Red=Cloning site Green=Tags(s)
  
  MAGNCGARGALSAHTLLFDLPPALLGELCAVLDSCDGALGWRGLAERLSSSWLDVRHIEKYVDQGKSGTR
  ELLWSWAQKNKTIGDLLQVLQEMGHRRAIHLITNYGAVLSPSEKSYQEGGFPNILFKETANVTVDNVLIP
  EHNEKGVLLKSSISFQNIIEGTRNFHKDFLIGEGEIFEVYRVEIQNLTYAVKLFKQEKKMQCKKHWKRFL
  SELEVLLLFHHPNILELAAYFTETEKFCLIYPYMRNGTLFDRLQCVGDTAPLPWHIRIGILIGISKAIHY
  LHNVQPCSVICGSISSANILLDDQFQPKLTDFAMAHFRSHLEHQSCTINMTSSSSKHLWYMPEEYIRQGK
  LSIKTDVYSFGIVIMEVLTGCRVVLDDPKHIQLRDLLRELMEKRGLDSCLSFLDKKVPPCPRNFSAKLFC
  LAGRCAATRAKLRPSMDEVLNTLESTQASLYFAEDPPTSLKSFRCPSPLFLENVPSIPVEDDESQNNNLL
  PSDEGLRIDRMTQKTPFECSQSEVMFLSLDKKPESKRNEEACNMPSSSCEESWFPKYIVPSQDLRPYKVN
  IDPSSEAPGHSCRSRPVESSCSSKFSWDEYEQYKKE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007199
• ORF Size: 1788 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007199.1, NP_009130.1
• RefSeq Size: 2288
• RefSeq ORF: 1791
• Locus ID: 11213
• Domains: DEATH, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Neurotrophin signaling pathway
• Gene Summary: This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]