SF3B4 (NM_005850) Human Tagged ORF Clone

CAT#: RG210352

SF3B4 (GFP-tagged) - Human splicing factor 3b, subunit 4, 49kDa (SF3B4)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SF3B4
• Synonyms: AFD1; Hsh49; SAP49; SF3b49
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210352 representing NM_005850
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCGGGCCGATCTCCGAGCGGAATCAGGATGCCACTGTGTACGTGGGGGGCCTGGATGAGAAGG
  TTAGTGAACCGCTGCTGTGGGAACTGTTTCTCCAGGCTGGACCAGTAGTCAACACCCACATGCCAAAGGA
  TAGAGTCACTGGCCAGCACCAAGGCTATGGCTTTGTGGAATTCTTGAGTGAGGAAGATGCTGACTATGCC
  ATTAAGATCATGAACATGATCAAACTCTATGGGAAGCCAATACGGGTGAACAAAGCATCAGCTCACAACA
  AAAACCTGGATGTAGGGGCCAACATTTTCATTGGGAACCTGGACCCTGAGATTGATGAGAAGTTGCTTTA
  TGATACTTTCAGCGCCTTTGGGGTCATCTTACAAACCCCCAAAATTATGCGGGACCCTGACACAGGCAAC
  TCCAAAGGTTATGCCTTTATTAATTTTGCTTCATTTGATGCTTCGGATGCAGCAATTGAAGCCATGAATG
  GGCAGTACCTCTGTAACCGTCCTATCACCGTATCTTATGCCTTCAAGAAGGACTCCAAGGGTGAGCGCCA
  TGGCTCAGCAGCCGAACGACTTCTGGCAGCTCAGAACCCGCTCTCCCAGGCTGATCGCCCTCATCAGCTG
  TTTGCAGATGCACCTCCTCCACCCTCTGCTCCCAATCCTGTGGTATCATCATTGGGGTCTGGGCTTCCTC
  CACCAGGCATGCCTCCTCCTGGCTCCTTCCCACCCCCAGTGCCACCTCCTGGAGCCCTCCCACCTGGGAT
  ACCCCCAGCCATGCCCCCACCACCTATGCCTCCTGGGGCTGCAGGACATGGCCCCCCATCGGCAGGAACC
  CCAGGGGCAGGACATCCTGGTCATGGACACTCACATCCTCACCCATTCCCACCGGGTGGGATGCCCCATC
  CAGGGATGTCTCAGATGCAGCTTGCACACCATGGCCCTCATGGCTTAGGACATCCCCACGCTGGACCCCC
  AGGCTCTGGGGGCCAGCCACCGCCCCGACCACCACCTGGAATGCCTCATCCTGGACCTCCTCCAATGGGC
  ATGCCCCCCCGAGGGCCTCCATTCGGATCTCCCATGGGTCACCCAGGTCCTATGCCTCCGCATGGTATGC
  GTGGACCTCCTCCACTGATGCCCCCCCATGGATACACTGGCCCTCCACGACCCCCACCCTATGGCTACCA
  GCGGGGGCCTCTCCCTCCACCCAGACCCACTCCCCGGCCACCAGTTCCCCCTCGAGGCCCACTTCGAGGC
  CCTCTCCCTCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210352 representing NM_005850
  Red=Cloning site Green=Tags(s)
  
  MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYA
  IKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGN
  SKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQL
  FADAPPPPSAPNPVVSSLGSGLPPPGMPPPGSFPPPVPPPGALPPGIPPAMPPPPMPPGAAGHGPPSAGT
  PGAGHPGHGHSHPHPFPPGGMPHPGMSQMQLAHHGPHGLGHPHAGPPGSGGQPPPRPPPGMPHPGPPPMG
  MPPRGPPFGSPMGHPGPMPPHGMRGPPPLMPPHGYTGPPRPPPYGYQRGPLPPPRPTPRPPVPPRGPLRG
  PLPQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005850
• ORF Size: 1272 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_005850.4, NP_005841.1
• RefSeq Size: 2001
• RefSeq ORF: 1275
• Locus ID: 10262
• Domains: RRM
• Protein Pathways: Spliceosome
• Gene Summary: This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq, Jul 2008]