NCKAP1L (NM_005337) Human Tagged ORF Clone

CAT#: RG210832

NCKAP1L (GFP-tagged) - Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NCKAP1L
• Synonyms: HEM1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210832 representing NM_005337
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTTGACATCTGCTTACCAGCATAAATTAGCAGAGAAGCTCACTATCCTGAATGATCGCGGTCAGG
  GGGTTCTCATCCGTATGTATAACATCAAGAAGACTTGTTCAGACCCCAAATCTAAGCCACCTTTCTTACT
  GGAAAAGTCCATGGAACCATCTCTCAAGTATATCAACAAGAAATTTCCCAACATAGATGTCCGAAACAGC
  ACGCAACATTTAGGACCAGTACATCGTGAAAAAGCCGAGATAATTAGATTCCTCACCAACTACTACCAGT
  CATTTGTGGATGTCATGGAATTTCGGGATCATGTATATGAACTTCTCAACACCATTGATGCCTGCCAGTG
  CCATTTTGATATCAATCTCAACTTTGATTTCACTCGGAGTTACCTGGACTTGATTGTAACTTACACCTCA
  GTCATTTTACTTCTGTCACGGATTGAAGATCGGCGGATACTCATTGGCATGTACAATTGTGCCCATGAGA
  TGCTGCATGGGCATGGTGACCCCAGTTTTGCCCGTCTGGGTCAGATGGTCTTGGAGTATGACCACCCTCT
  GAAGAAGCTGACAGAAGAGTTTGGGCCTCACACAAAGGCTGTGAGTGGAGCCCTCCTCTCTTTGCATTTC
  CTCTTTGTCCGAAGAAACCAGGGGGCTGAGCAGTGGCGCAGTGCCCAACTTCTAAGCCTCATCAGCAACC
  CCCCAGCCATGATTAACCCTGCTAATTCAGATACAATGGCCTGTGAGTATCTGTCTGTGGAAGTAATGGA
  GCGCTGGATTATCATTGGGTTTCTTCTTTGTCATGGGTGCCTCAACTCCAATAGCCAGTGCCAGAAGCTG
  TGGAAGCTGTGTCTGCAGGGCTCCCTCTACATCACCCTTATCCGTGAGGATGTGCTGCAGGTGCACAAAG
  TCACCGAGGACCTGTTTAGCAGTTTGAAAGGGTATGGCAAGAGAGTGGCAGACATAAAGGAGAGCAAGGA
  ACATGTAATTGCAAACAGTGGCCAGTTTCATTGTCAACGGCGGCAATTTCTGCGGATGGCAGTGAAGGAG
  CTGGAGACTGTGTTGGCTGATGAACCGGGACTACTGGGTCCTAAGGCTCTTTTTGCTTTCATGGCCCTGT
  CCTTCATTCGTGATGAGGTCACCTGGCTGGTTCGCCACACAGAGAATGTCACCAAGACAAAGACACCTGA
  GGACTATGCTGACTCGAGCATTGCAGAGCTACTTTTCTTGTTGGAGGGGATTAGGTCTCTGGTCCGAAGA
  CACATCAAAGTGATACAGCAATACCACCTTCAGTACTTGGCAAGATTTGATGCTCTTGTGCTCAGTGACA
  TCATTCAGAACTTGTCTGTGTGTCCAGAGGAGGAGTCCATCATCATGTCCTCATTCGTCAGTATCCTCTC
  CTCTCTGAATCTCAAACAAGTTGATAATGGAGAAAAATTTGAATTCTCAGGATTGAGGCTGGACTGGTTC
  CGCCTACAGGCATACACTAGCGTGGCTAAGGCCCCTCTGCACCTGCATGAGAACCCTGACTTAGCCAAGG
  TGATGAACCTCATTGTCTTCCACTCCCGAATGCTGGACTCCGTAGAAAAATTGCTGGTGGAAACTTCTGA
  TCTGTCTACTTTCTGCTTTCATCTTCGTATCTTTGAGAAGATGTTTGCCATGACCTTGGAGGAATCTGCC
  ATGTTGCGTTATGCCATTGCTTTCCCCCTGATTTGTGCTCACTTTGTCCACTGCACTCATGAGATGTGCC
  CAGAGGAGTACCCCCACCTCAAGAACCATGGTCTTCACCACTGCAACTCCTTCCTGGAAGAGTTGGCCAA
  GCAGACCAGCAATTGCGTCCTGGAGATCTGTGCTGAGCAGCGAAACCTGAGCGAGCAGCTTCTACCTAAG
  CACTGTGCCACTACAATCAGCAAAGCCAAGAACAAGAAAACCAGGAAGCAGAGGCAGACTCCCAGAAAAG
  GAGAGCCCGAGAGGGACAAGCCAGGAGCTGAGAGTCACCGGAAGAACCGCAGCATTGTCACCAACATGGA
  CAAGCTACACCTAAACTTGACAGAACTGGCACTGACAATGAATCATGTATACAGTTTCTCCGTGTTTGAA
  CATACTATCTTCCCTTCTGAGTACCTCAGCAGCCACCTGGAGGCCAGACTCAACAGAGCCATTGTGTGGC
  TGGCTGGCTACAATGCCACGACCCAGGAGATCGTACGGCCTTCTGAGCTGTTGGCAGGAGTCAAAGCATA
  CATTGGTTTCATACAGTCACTGGCCCAGTTTTTGGGTGCAGATGCTTCCAGAGTCATCCGCAACGCCCTC
  CTGCAGCAGACACAACCACTGGATTCCTGTGGGGAACAGACAATCACCACACTCTACACAAACTGGTACC
  TGGAAAGTCTGCTTAGACAGGCAAGCAGTGGGACCATCATCCTCTCCCCAGCCATGCAGGCCTTCGTCAG
  CCTGCCCAGAGAAGGGGAGCAGAACTTCAGTGCAGAGGAGTTCTCTGACATCTCTGAGATGCGGGCCTTG
  GCAGAACTCCTGGGCCCCTATGGAATGAAGTTCCTGAGTGAAAACCTGATGTGGCATGTGACCTCTCAGA
  TTGTGGAGCTGAAGAAGCTGGTGGTGGAAAACATGGACATACTTGTTCAGATCAGATCCAACTTTAGCAA
  GCCGGACTTGATGGCTTCCCTGCTGCCCCAGCTGACAGGGGCTGAAAATGTGCTAAAGCGCATGACCATC
  ATTGGGGTTATCCTCAGTTTCAGGGCCATGGCCCAAGAGGGACTTCGGGAGGTTTTCTCCTCCCACTGCC
  CATTTCTTATGGGTCCCATTGAGTGCTTGAAGGAGTTTGTCACTCCAGACACAGACATCAAGGTGACCTT
  GAGTATCTTTGAGCTGGCATCTGCTGCAGGTGTGGGCTGTGACATTGACCCAGCCTTGGTGGCTGCCATT
  GCTAATCTGAAAGCTGATACTTCATCTCCTGAGGAGGAATATAAGGTGGCCTGCCTGCTCTTGATCTTTC
  TGGCAGTTTCCCTCCCACTCCTTGCCACTGACCCTTCTTCCTTTTATAGCATTGAGAAGGATGGTTACAA
  CAACAATATTCATTGCTTGACCAAAGCCATCATCCAGGTGTCTGCTGCCCTCTTCACGCTCTACAACAAG
  AACATTGAAACTCACCTCAAGGAATTTCTGGTGGTGGCCTCTGTCAGCCTCTTGCAGCTGGGCCAGGAGA
  CTGACAAGCTTAAAACCAGAAATCGAGAATCCATTTCTCTGCTCATGCGCTTGGTGGTGGAGGAGTCATC
  CTTCCTGACCCTGGACATGCTGGAGTCCTGTTTCCCTTATGTCCTGCTTCGAAATGCCTATCGGGAGGTG
  TCTCGGGCCTTCCACCTAAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210832 representing NM_005337
  Red=Cloning site Green=Tags(s)
  
  MSLTSAYQHKLAEKLTILNDRGQGVLIRMYNIKKTCSDPKSKPPFLLEKSMEPSLKYINKKFPNIDVRNS
  TQHLGPVHREKAEIIRFLTNYYQSFVDVMEFRDHVYELLNTIDACQCHFDINLNFDFTRSYLDLIVTYTS
  VILLLSRIEDRRILIGMYNCAHEMLHGHGDPSFARLGQMVLEYDHPLKKLTEEFGPHTKAVSGALLSLHF
  LFVRRNQGAEQWRSAQLLSLISNPPAMINPANSDTMACEYLSVEVMERWIIIGFLLCHGCLNSNSQCQKL
  WKLCLQGSLYITLIREDVLQVHKVTEDLFSSLKGYGKRVADIKESKEHVIANSGQFHCQRRQFLRMAVKE
  LETVLADEPGLLGPKALFAFMALSFIRDEVTWLVRHTENVTKTKTPEDYADSSIAELLFLLEGIRSLVRR
  HIKVIQQYHLQYLARFDALVLSDIIQNLSVCPEEESIIMSSFVSILSSLNLKQVDNGEKFEFSGLRLDWF
  RLQAYTSVAKAPLHLHENPDLAKVMNLIVFHSRMLDSVEKLLVETSDLSTFCFHLRIFEKMFAMTLEESA
  MLRYAIAFPLICAHFVHCTHEMCPEEYPHLKNHGLHHCNSFLEELAKQTSNCVLEICAEQRNLSEQLLPK
  HCATTISKAKNKKTRKQRQTPRKGEPERDKPGAESHRKNRSIVTNMDKLHLNLTELALTMNHVYSFSVFE
  HTIFPSEYLSSHLEARLNRAIVWLAGYNATTQEIVRPSELLAGVKAYIGFIQSLAQFLGADASRVIRNAL
  LQQTQPLDSCGEQTITTLYTNWYLESLLRQASSGTIILSPAMQAFVSLPREGEQNFSAEEFSDISEMRAL
  AELLGPYGMKFLSENLMWHVTSQIVELKKLVVENMDILVQIRSNFSKPDLMASLLPQLTGAENVLKRMTI
  IGVILSFRAMAQEGLREVFSSHCPFLMGPIECLKEFVTPDTDIKVTLSIFELASAAGVGCDIDPALVAAI
  ANLKADTSSPEEEYKVACLLLIFLAVSLPLLATDPSSFYSIEKDGYNNNIHCLTKAIIQVSAALFTLYNK
  NIETHLKEFLVVASVSLLQLGQETDKLKTRNRESISLLMRLVVEESSFLTLDMLESCFPYVLLRNAYREV
  SRAFHLN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005337
• ORF Size: 3381 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005337.3, NP_005328.2
• RefSeq Size: 3869 bp
• RefSeq ORF: 3384 bp
• Locus ID: 3071
• Cytogenetics: 12q13.13-q13.2
• Protein Pathways: Regulation of actin cytoskeleton
• Gene Summary: 'This gene encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and plants. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010]'