NCKAP1L (NM_005337) Human Untagged Clone

CAT#: SC318508

NCKAP1L (untagged)-Human NCK-associated protein 1-like (NCKAP1L), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCKAP1L
• Synonyms: HEM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene SC318508 ORF sequence for NM_005337, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTTTGACATCTGCTTACCAGCATAAATTAGCAGAGAAGCTCACTATCCTGAATGATCGCGGTCAGG
  GGGTTCTCATCCGTATGTATAACATCAAGAAGACTTGTTCAGACCCCAAATCTAAGCCACCTTTCTTACT
  GGAAAAGTCCATGGAACCATCTCTCAAGTATATCAACAAGAAATTTCCCAACATAGATGTCCGAAACAGC
  ACGCAACATTTAGGACCAGTACATCGTGAAAAAGCCGAGATAATTAGATTCCTCACCAACTACTACCAGT
  CATTTGTGGATGTCATGGAATTTCGGGATCATGTATATGAACTTCTCAACACCATTGATGCCTGCCAGTG
  CCATTTTGATATCAATCTCAACTTTGATTTCACTCGGAGTTACCTGGACTTGATTGTAACTTACACCTCA
  GTCATTTTACTTCTGTCACGGATTGAAGATCGGCGGATACTCATTGGCATGTACAATTGTGCCCATGAGA
  TGCTGCATGGGCATGGTGACCCCAGTTTTGCCCGTCTGGGTCAGATGGTCTTGGAGTATGACCACCCTCT
  GAAGAAGCTGACAGAAGAGTTTGGGCCTCACACAAAGGCTGTGAGTGGAGCCCTCCTCTCTTTGCATTTC
  CTCTTTGTCCGAAGAAACCAGGGGGCTGAGCAGTGGCGCAGTGCCCAACTTCTAAGCCTCATCAGCAACC
  CCCCAGCCATGATTAACCCTGCTAATTCAGATACAATGGCCTGTGAGTATCTGTCTGTGGAAGTAATGGA
  GCGCTGGATTATCATTGGGTTTCTTCTTTGTCATGGGTGCCTCAACTCCAATAGCCAGTGCCAGAAGCTG
  TGGAAGCTGTGTCTGCAGGGCTCCCTCTACATCACCCTTATCCGTGAGGATGTGCTGCAGGTGCACAAAG
  TCACCGAGGACCTGTTTAGCAGTTTGAAAGGGTATGGCAAGAGAGTGGCAGACATAAAGGAGAGCAAGGA
  ACATGTAATTGCAAACAGTGGCCAGTTTCATTGTCAACGGCGGCAATTTCTGCGGATGGCAGTGAAGGAG
  CTGGAGACTGTGTTGGCTGATGAACCGGGACTACTGGGTCCTAAGGCTCTTTTTGCTTTCATGGCCCTGT
  CCTTCATTCGTGATGAGGTCACCTGGCTGGTTCGCCACACAGAGAATGTCACCAAGACAAAGACACCTGA
  GGACTATGCTGACTCGAGCATTGCAGAGCTACTTTTCTTGTTGGAGGGGATTAGGTCTCTGGTCCGAAGA
  CACATCAAAGTGATACAGCAATACCACCTTCAGTACTTGGCAAGATTTGATGCTCTTGTGCTCAGTGACA
  TCATTCAGAACTTGTCTGTGTGTCCAGAGGAGGAGTCCATCATCATGTCCTCATTCGTCAGTATCCTCTC
  CTCTCTGAATCTCAAACAAGTTGATAATGGAGAAAAATTTGAATTCTCAGGATTGAGGCTGGACTGGTTC
  CGCCTACAGGCATACACTAGCGTGGCTAAGGCCCCTCTGCACCTGCATGAGAACCCTGACTTAGCCAAGG
  TGATGAACCTCATTGTCTTCCACTCCCGAATGCTGGACTCCGTAGAAAAATTGCTGGTGGAAACTTCTGA
  TCTGTCTACTTTCTGCTTTCATCTTCGTATCTTTGAGAAGATGTTTGCCATGACCTTGGAGGAATCTGCC
  ATGTTGCGTTATGCCATTGCTTTCCCCCTGATTTGTGCTCACTTTGTCCACTGCACTCATGAGATGTGCC
  CAGAGGAGTACCCCCACCTCAAGAACCATGGTCTTCACCACTGCAACTCCTTCCTGGAAGAGTTGGCCAA
  GCAGACCAGCAATTGCGTCCTGGAGATCTGTGCTGAGCAGCGAAACCTGAGCGAGCAGCTTCTACCTAAG
  CACTGTGCCACTACAATCAGCAAAGCCAAGAACAAGAAAACCAGGAAGCAGAGGCAGACTCCCAGAAAAG
  GAGAGCCCGAGAGGGACAAGCCAGGAGCTGAGAGTCACCGGAAGAACCGCAGCATTGTCACCAACATGGA
  CAAGCTACACCTAAACTTGACAGAACTGGCACTGACAATGAATCATGTATACAGTTTCTCCGTGTTTGAA
  CATACTATCTTCCCTTCTGAGTACCTCAGCAGCCACCTGGAGGCCAGACTCAACAGAGCCATTGTGTGGC
  TGGCTGGCTACAATGCCACGACCCAGGAGATCGTACGGCCTTCTGAGCTGTTGGCAGGAGTCAAAGCATA
  CATTGGTTTCATACAGTCACTGGCCCAGTTTTTGGGTGCAGATGCTTCCAGAGTCATCCGCAACGCCCTC
  CTGCAGCAGACACAACCACTGGATTCCTGTGGGGAACAGACAATCACCACACTCTACACAAACTGGTACC
  TGGAAAGTCTGCTTAGACAGGCAAGCAGTGGGACCATCATCCTCTCCCCAGCCATGCAGGCCTTCGTCAG
  CCTGCCCAGAGAAGGGGAGCAGAACTTCAGTGCAGAGGAGTTCTCTGACATCTCTGAGATGCGGGCCTTG
  GCAGAACTCCTGGGCCCCTATGGCATGAAGTTCCTGAGTGAAAACCTGATGTGGCATGTGACCTCTCAGA
  TTGTGGAGCTGAAGAAGCTGGTGGTGGAAAACATGGACATACTTGTTCAGATCAGATCCAACTTTAGCAA
  GCCGGACTTGATGGCTTCCCTGCTGCCCCAGCTGACAGGGGCTGAAAATGTGCTAAAGCGCATGACCATC
  ATTGGGGTTATCCTCAGTTTCAGGGCCATGGCCCAAGAGGGACTTCGGGAGGTTTTCTCCTCCCACTGCC
  CATTTCTTATGGGTCCCATTGAGTGCTTGAAGGAGTTTGTCACTCCAGACACAGACATCAAGGTGACCTT
  GAGTATCTTTGAGCTGGCATCTGCTGCAGGTGTGGGCTGTGACATTGACCCAGCCTTGGTGGCTGCCATT
  GCTAATCTGAAAGCTGATACTTCATCTCCTGAGGAGGAATATAAGGTGGCCTGCCTGCTCTTGATCTTTC
  TGGCAGTTTCCCTCCCACTCCTTGCCACTGACCCTTCTTCCTTTTATAGCATTGAGAAGGATGGTTACAA
  CAACAATATTCATTGCTTGACCAAAGCCATCATCCAGGTGTCTGCTGCCCTCTTCACGCTCTACAACAAG
  AACATTGAAACTCACCTCAAGGAATTTCTGGTGGTGGCCTCTGTCAGCCTCTTGCAGCTGGGCCAGGAGA
  CTGACAAGCTTAAAACCAGAAATCGAGAATCCATTTCTCTGCTCATGCGCTTGGTGGTGGAGGAGTCATC
  CTTCCTGACCCTGGACATGCTGGAGTCCTGTTTCCCTTATGTCCTGCTTCGAAATGCCTATCGGGAGGTG
  TCTCGGGCCTTCCACCTAAACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_005337
• Insert Size: 3909 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005337.3, NP_005328.2
• RefSeq Size: 3909 bp
• RefSeq ORF: 3384 bp
• Locus ID: 3071
• Cytogenetics: 12q13.13-q13.2
• Protein Pathways: Regulation of actin cytoskeleton
• Gene Summary: 'This gene encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and plants. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).