IL1RAP (NM_002182) Human Tagged ORF Clone

CAT#: RG211970

IL1RAP (GFP-tagged) - Human interleukin 1 receptor accessory protein (IL1RAP), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: IL1RAP
• Synonyms: C3orf13; IL-1RAcP; IL1R3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211970 representing NM_002182
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACACTTCTGTGGTGTGTAGTGAGTCTCTACTTTTATGGAATCCTGCAAAGTGATGCCTCAGAACGCT
  GCGATGACTGGGGACTAGACACCATGAGGCAAATCCAAGTGTTTGAAGATGAGCCAGCTCGCATCAAGTG
  CCCACTCTTTGAACACTTCTTGAAATTCAACTACAGCACAGCCCATTCAGCTGGCCTTACTCTGATCTGG
  TATTGGACTAGGCAGGACCGGGACCTTGAGGAGCCAATTAACTTCCGCCTCCCCGAGAACCGCATTAGTA
  AGGAGAAAGATGTGCTGTGGTTCCGGCCCACTCTCCTCAATGACACTGGCAACTATACCTGCATGTTAAG
  GAACACTACATATTGCAGCAAAGTTGCATTTCCCTTGGAAGTTGTTCAAAAAGACAGCTGTTTCAATTCC
  CCCATGAAACTCCCAGTGCATAAACTGTATATAGAATATGGCATTCAGAGGATCACTTGTCCAAATGTAG
  ATGGATATTTTCCTTCCAGTGTCAAACCGACTATCACTTGGTATATGGGCTGTTATAAAATACAGAATTT
  TAATAATGTAATACCCGAAGGTATGAACTTGAGTTTCCTCATTGCCTTAATTTCAAATAATGGAAATTAC
  ACATGTGTTGTTACATATCCAGAAAATGGACGTACGTTTCATCTCACCAGGACTCTGACTGTAAAGGTAG
  TAGGCTCTCCAAAAAATGCAGTGCCCCCTGTGATCCATTCACCTAATGATCATGTGGTCTATGAGAAAGA
  ACCAGGAGAGGAGCTACTCATTCCCTGTACGGTCTATTTTAGTTTTCTGATGGATTCTCGCAATGAGGTT
  TGGTGGACCATTGATGGAAAAAAACCTGATGACATCACTATTGATGTCACCATTAACGAAAGTATAAGTC
  ATAGTAGAACAGAAGATGAAACAAGAACTCAGATTTTGAGCATCAAGAAAGTTACCTCTGAGGATCTCAA
  GCGCAGCTATGTCTGTCATGCTAGAAGTGCCAAAGGCGAAGTTGCCAAAGCAGCCAAGGTGAAGCAGAAA
  GTGCCAGCTCCAAGATACACAGTGGAACTGGCTTGTGGTTTTGGAGCCACAGTCCTGCTAGTGGTGATTC
  TCATTGTTGTTTACCATGTTTACTGGCTAGAGATGGTCCTATTTTACCGGGCTCATTTTGGAACAGATGA
  AACCATTTTAGATGGAAAAGAGTATGATATTTATGTATCCTATGCAAGGAATGCGGAAGAAGAAGAATTT
  GTATTACTGACCCTCCGTGGAGTTTTGGAGAATGAATTTGGATACAAGCTGTGCATCTTTGACCGAGACA
  GTCTGCCTGGGGGAATTGTCACAGATGAGACTTTGAGCTTCATTCAGAAAAGCAGACGCCTCCTGGTTGT
  TCTAAGCCCCAACTACGTGCTCCAGGGAACCCAAGCCCTCCTGGAGCTCAAGGCTGGCCTAGAAAATATG
  GCCTCTCGGGGCAACATCAACGTCATTTTAGTACAGTACAAAGCTGTGAAGGAAACGAAGGTGAAAGAGC
  TGAAGAGGGCTAAGACGGTGCTCACGGTCATTAAATGGAAAGGGGAAAAATCCAAGTATCCACAGGGCAG
  GTTCTGGAAGCAGCTGCAGGTGGCCATGCCAGTGAAGAAAAGTCCCAGGCGGTCTAGCAGTGATGAGCAG
  GGCCTCTCGTATTCATCTTTGAAAAATGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211970 representing NM_002182
  Red=Cloning site Green=Tags(s)
  
  MTLLWCVVSLYFYGILQSDASERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKFNYSTAHSAGLTLIW
  YWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNS
  PMKLPVHKLYIEYGIQRITCPNVDGYFPSSVKPTITWYMGCYKIQNFNNVIPEGMNLSFLIALISNNGNY
  TCVVTYPENGRTFHLTRTLTVKVVGSPKNAVPPVIHSPNDHVVYEKEPGEELLIPCTVYFSFLMDSRNEV
  WWTIDGKKPDDITIDVTINESISHSRTEDETRTQILSIKKVTSEDLKRSYVCHARSAKGEVAKAAKVKQK
  VPAPRYTVELACGFGATVLLVVILIVVYHVYWLEMVLFYRAHFGTDETILDGKEYDIYVSYARNAEEEEF
  VLLTLRGVLENEFGYKLCIFDRDSLPGGIVTDETLSFIQKSRRLLVVLSPNYVLQGTQALLELKAGLENM
  ASRGNINVILVQYKAVKETKVKELKRAKTVLTVIKWKGEKSKYPQGRFWKQLQVAMPVKKSPRRSSSDEQ
  GLSYSSLKNV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002182
• ORF Size: 1710 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002182.2, NP_002173.1
• RefSeq Size: 4726 bp
• RefSeq ORF: 1713 bp
• Locus ID: 3556
• Cytogenetics: 3q28
• Domains: TIR, ig, IG
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Apoptosis, Cytokine-cytokine receptor interaction
• Gene Summary: 'This gene encodes a component of the interleukin 1 receptor complex, which initiates signalling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in membrane-bound and soluble isoforms differing in their C-terminus. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress. [provided by RefSeq, Jul 2018]'