DNASE2B (NM_021233) Human Tagged ORF Clone

CAT#: RG212192

DNASE2B (GFP-tagged) - Human deoxyribonuclease II beta (DNASE2B), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DNASE2B
• Synonyms: DLAD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212192 representing NM_021233
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCAAGACTGCTAAGAACATCCTTTGCTTTGCTCTTCCTTGGCCTCTTTGGGGTGCTGGGGGCAG
  CAACAATTTCATGCAGAAATGAAGAAGGGAAAGCTGTGGACTGGTTTACTTTTTATAAGTTACCTAAAAA
  ACAAAACAAGGAAAGTGGAGAGACTGGGTTAGAGTACCTGTACCTAGACTCTACAACTAGAAGCTGGAGG
  AAGAGTGAGCAACTAATGAATGACACCAAGAGTGTTTTGGGAAGGACATTACAACAGCTATATGAAGCAT
  ATGCCTCTAAGAGTAACAACACAGCCTATCTAATATACAATGATGGAGTCCCTAAACCTGTGAATTACAG
  CAGAAAGTATGGACACACCAAAGGTTTACTGCTGTGGAACAGAGTTCAAGGGTTCTGGCTGATTCATTCC
  ATCCCTCAGTTTCCTCCAATTCCGGAAGAAGGCTATGATTATCCACCCACAGGGAGACGAAATGGACAAA
  GTGGCATCTGCATAACTTTCAAGTACAACCAGTATGAGGCAATAGATTCTCAGCTCTTGGTCTGCAACCC
  CAACGTCTATAGCTGCTCCATCCCAGCCACCTTTCACCAGGAGCTCATTCACATGCCCCAGCTGTGCACC
  AGGGCCAGCTCATCAGAGATTCCTGGCAGGCTCCTCACCACACTTCAGTCGGCCCAGGGACAAAAATTCC
  TCCATTTTGCAAAGTCGGATTCTTTTCTTGACGACATCTTTGCAGCCTGGATGGCTCAACGGCTGAAGAC
  ACACTTGTTAACAGAAACCTGGCAGCGAAAAAGACAAGAGCTTCCTTCAAACTGCTCCCTTCCTTACCAT
  GTCTACAATATAAAAGCAATTAAATTATCACGACACTCTTATTTCAGTTCTTATCAAGATCACGCCAAGT
  GGTGTATTTCCCAAAAGGGCACCAAAAATCGCTGGACATGTATTGGAGACCTAAATCGGAGTCCACACCA
  AGCCTTCAGAAGTGGAGGATTCATTTGTACCCAGAATTGGCAAATTTACCAAGCATTTCAAGGATTAGTA
  TTATACTATGAAAGCTGTAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212192 representing NM_021233
  Red=Cloning site Green=Tags(s)
  
  MMARLLRTSFALLFLGLFGVLGAATISCRNEEGKAVDWFTFYKLPKKQNKESGETGLEYLYLDSTTRSWR
  KSEQLMNDTKSVLGRTLQQLYEAYASKSNNTAYLIYNDGVPKPVNYSRKYGHTKGLLLWNRVQGFWLIHS
  IPQFPPIPEEGYDYPPTGRRNGQSGICITFKYNQYEAIDSQLLVCNPNVYSCSIPATFHQELIHMPQLCT
  RASSSEIPGRLLTTLQSAQGQKFLHFAKSDSFLDDIFAAWMAQRLKTHLLTETWQRKRQELPSNCSLPYH
  VYNIKAIKLSRHSYFSSYQDHAKWCISQKGTKNRWTCIGDLNRSPHQAFRSGGFICTQNWQIYQAFQGLV
  LYYESCK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021233
• ORF Size: 1071 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021233.2, NP_067056.2
• RefSeq Size: 1279
• RefSeq ORF: 1086
• Locus ID: 58511
• Protein Families: Transmembrane
• Protein Pathways: Lysosome
• Gene Summary: The protein encoded by this gene shares considerable sequence similarity to, and is structurally related to DNase II. The latter is a well characterized endonuclease that catalyzes DNA hydrolysis in the absence of divalent cations at acidic pH. Unlike DNase II which is ubiquitously expressed, expression of this gene product is restricted to the salivary gland and lungs. The gene has been localized to chromosome 1p22.3 adjacent (and in opposite orientation) to the uricase pseudogene. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]